Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces

G Khodakaramian, S Lissenden, B Gust, L Moir, P A Hoskisson, K F Chater, M C M Smith

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phi C31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or PgI in S. coelicolor. Cre-phage was also used for marker deletion in other strains of S. coelicolor.

Original languageEnglish
Number of pages5
JournalNucleic Acids Research
Volume34
DOIs
Publication statusPublished - 2006

Keywords

  • COELICOLOR A3(2)
  • LYTIC DEVELOPMENT
  • ESCHERICHIA-COLI
  • EARLY REGION
  • PHI-C31
  • SEQUENCE
  • CLONING
  • GENOME
  • GENES
  • SITE

Cite this

Khodakaramian, G., Lissenden, S., Gust, B., Moir, L., Hoskisson, P. A., Chater, K. F., & Smith, M. C. M. (2006). Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces. Nucleic Acids Research, 34. https://doi.org/10.1093/nar/gnj019

Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces. / Khodakaramian, G ; Lissenden, S ; Gust, B ; Moir, L ; Hoskisson, P A ; Chater, K F ; Smith, M C M .

In: Nucleic Acids Research, Vol. 34, 2006.

Research output: Contribution to journalArticle

Khodakaramian, G ; Lissenden, S ; Gust, B ; Moir, L ; Hoskisson, P A ; Chater, K F ; Smith, M C M . / Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces. In: Nucleic Acids Research. 2006 ; Vol. 34.
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AU - Chater, K F

AU - Smith, M C M

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AB - We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phi C31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or PgI in S. coelicolor. Cre-phage was also used for marker deletion in other strains of S. coelicolor.

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