Expression of CYP2S1 in human hepatic stellate cells

Carylyn Jane Marek, Steven J. Tucker, Matthew Koruth, Karen Wallace, Matthew Christopher Wright

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Activated stellate cells are myofibroblast-like cells associated with the generation of fibrotic searing in chronically damaged liver. Gene chip analysis was performed on cultured fibrotic stellate cells. Of the 51 human CYP genes known, 13 CYP and 5 CYP reduction-related genes were detected with 4 CYPs (CYP1A1, CYP2E1, CY2S1 and CYP4F3) consistently present in stellate cells isolated from three individuals. Quantitative RT-PCR indicated that CYP2S1 was a major expressed CYP mRNA transcript. The presence of a CYP2A-related protein and testosterone metabolism in stellate cell cultures suggest that stellate cells express specific functional isoforms of CYP of which a major form is CYP2S1. (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)781-786
Number of pages6
JournalFEBS Letters
Volume581
Issue number4
Early online date2 Feb 2007
DOIs
Publication statusPublished - 20 Feb 2007

Keywords

  • cytochrome P450
  • liver
  • fibrosis
  • CYP1B1
  • CYP4F3
  • retinoids
  • cytochrome P450CYP2S1
  • localization
  • activation
  • mechanism
  • PXR

Cite this

Marek, C. J., Tucker, S. J., Koruth, M., Wallace, K., & Wright, M. C. (2007). Expression of CYP2S1 in human hepatic stellate cells. FEBS Letters, 581(4), 781-786. https://doi.org/10.1016/j.febslet.2007.01.056

Expression of CYP2S1 in human hepatic stellate cells. / Marek, Carylyn Jane; Tucker, Steven J.; Koruth, Matthew; Wallace, Karen; Wright, Matthew Christopher.

In: FEBS Letters, Vol. 581, No. 4, 20.02.2007, p. 781-786.

Research output: Contribution to journalArticle

Marek, CJ, Tucker, SJ, Koruth, M, Wallace, K & Wright, MC 2007, 'Expression of CYP2S1 in human hepatic stellate cells', FEBS Letters, vol. 581, no. 4, pp. 781-786. https://doi.org/10.1016/j.febslet.2007.01.056
Marek CJ, Tucker SJ, Koruth M, Wallace K, Wright MC. Expression of CYP2S1 in human hepatic stellate cells. FEBS Letters. 2007 Feb 20;581(4):781-786. https://doi.org/10.1016/j.febslet.2007.01.056
Marek, Carylyn Jane ; Tucker, Steven J. ; Koruth, Matthew ; Wallace, Karen ; Wright, Matthew Christopher. / Expression of CYP2S1 in human hepatic stellate cells. In: FEBS Letters. 2007 ; Vol. 581, No. 4. pp. 781-786.
@article{7a548847b06b4b598d75133be192ddff,
title = "Expression of CYP2S1 in human hepatic stellate cells",
abstract = "Activated stellate cells are myofibroblast-like cells associated with the generation of fibrotic searing in chronically damaged liver. Gene chip analysis was performed on cultured fibrotic stellate cells. Of the 51 human CYP genes known, 13 CYP and 5 CYP reduction-related genes were detected with 4 CYPs (CYP1A1, CYP2E1, CY2S1 and CYP4F3) consistently present in stellate cells isolated from three individuals. Quantitative RT-PCR indicated that CYP2S1 was a major expressed CYP mRNA transcript. The presence of a CYP2A-related protein and testosterone metabolism in stellate cell cultures suggest that stellate cells express specific functional isoforms of CYP of which a major form is CYP2S1. (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.",
keywords = "cytochrome P450, liver, fibrosis, CYP1B1, CYP4F3, retinoids, cytochrome P450CYP2S1, localization, activation, mechanism, PXR",
author = "Marek, {Carylyn Jane} and Tucker, {Steven J.} and Matthew Koruth and Karen Wallace and Wright, {Matthew Christopher}",
year = "2007",
month = "2",
day = "20",
doi = "10.1016/j.febslet.2007.01.056",
language = "English",
volume = "581",
pages = "781--786",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Expression of CYP2S1 in human hepatic stellate cells

AU - Marek, Carylyn Jane

AU - Tucker, Steven J.

AU - Koruth, Matthew

AU - Wallace, Karen

AU - Wright, Matthew Christopher

PY - 2007/2/20

Y1 - 2007/2/20

N2 - Activated stellate cells are myofibroblast-like cells associated with the generation of fibrotic searing in chronically damaged liver. Gene chip analysis was performed on cultured fibrotic stellate cells. Of the 51 human CYP genes known, 13 CYP and 5 CYP reduction-related genes were detected with 4 CYPs (CYP1A1, CYP2E1, CY2S1 and CYP4F3) consistently present in stellate cells isolated from three individuals. Quantitative RT-PCR indicated that CYP2S1 was a major expressed CYP mRNA transcript. The presence of a CYP2A-related protein and testosterone metabolism in stellate cell cultures suggest that stellate cells express specific functional isoforms of CYP of which a major form is CYP2S1. (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

AB - Activated stellate cells are myofibroblast-like cells associated with the generation of fibrotic searing in chronically damaged liver. Gene chip analysis was performed on cultured fibrotic stellate cells. Of the 51 human CYP genes known, 13 CYP and 5 CYP reduction-related genes were detected with 4 CYPs (CYP1A1, CYP2E1, CY2S1 and CYP4F3) consistently present in stellate cells isolated from three individuals. Quantitative RT-PCR indicated that CYP2S1 was a major expressed CYP mRNA transcript. The presence of a CYP2A-related protein and testosterone metabolism in stellate cell cultures suggest that stellate cells express specific functional isoforms of CYP of which a major form is CYP2S1. (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

KW - cytochrome P450

KW - liver

KW - fibrosis

KW - CYP1B1

KW - CYP4F3

KW - retinoids

KW - cytochrome P450CYP2S1

KW - localization

KW - activation

KW - mechanism

KW - PXR

U2 - 10.1016/j.febslet.2007.01.056

DO - 10.1016/j.febslet.2007.01.056

M3 - Article

VL - 581

SP - 781

EP - 786

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 4

ER -