Expression of CYP2S1 in human hepatic stellate cells

Carylyn Jane Marek, Steven J. Tucker, Matthew Koruth, Karen Wallace, Matthew Christopher Wright

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Activated stellate cells are myofibroblast-like cells associated with the generation of fibrotic searing in chronically damaged liver. Gene chip analysis was performed on cultured fibrotic stellate cells. Of the 51 human CYP genes known, 13 CYP and 5 CYP reduction-related genes were detected with 4 CYPs (CYP1A1, CYP2E1, CY2S1 and CYP4F3) consistently present in stellate cells isolated from three individuals. Quantitative RT-PCR indicated that CYP2S1 was a major expressed CYP mRNA transcript. The presence of a CYP2A-related protein and testosterone metabolism in stellate cell cultures suggest that stellate cells express specific functional isoforms of CYP of which a major form is CYP2S1. (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)781-786
Number of pages6
JournalFEBS Letters
Volume581
Issue number4
Early online date2 Feb 2007
DOIs
Publication statusPublished - 20 Feb 2007

Keywords

  • cytochrome P450
  • liver
  • fibrosis
  • CYP1B1
  • CYP4F3
  • retinoids
  • cytochrome P450CYP2S1
  • localization
  • activation
  • mechanism
  • PXR

Cite this

Marek, C. J., Tucker, S. J., Koruth, M., Wallace, K., & Wright, M. C. (2007). Expression of CYP2S1 in human hepatic stellate cells. FEBS Letters, 581(4), 781-786. https://doi.org/10.1016/j.febslet.2007.01.056