Expression of heparan sulfate proteoglycans in murine models of experimental colitis

A M Patterson, M I Delday, T H van Kuppevelt, G Loh, M Blaut, D Haller, G Grant, D Kelly

Research output: Contribution to journalArticle

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Abstract

BACKGROUND: Heparan sulfate proteoglycans (HSPGs) are considered important in maintaining physiological homeostasis in many systems. Their expression is altered greatly in several pathophysiological conditions. Herein, we assess the expression and cellular localization of HSPGs in two murine models of human inflammatory bowel disease (IBD). METHODS: Expression and localization of HSPGs, syndecans, and HS epitopes were examined in the colon of 129SvEv interleukin 10 knockout (IL10(-/-) ), C3Bir IL10(-/-) , and their genetic control (IL10(+/+) ) counterparts (129SvEv; C3H/HeJ). mRNA expression of syndecans and heparan sulfate biosynthesis enzymes were evaluated by real-time polymerase chain reaction (PCR). Localization of HSPGs was determined by immunofluorescence. RESULTS: mRNA for all syndecans was detected and expression in colonic tissues altered in IL10(-/-) mice. Syndecan-1 protein was expressed in the intestinal epithelium and on lamina propria cells of IL10(-/-) and control mice but was significantly reduced on the intestinal epithelial cells of IL10(-/-) , mice particularly with severe colitis. Syndecan-2 was not detected, whereas syndecan-3 immunoreactivity was localized in the lamina propria but did not differ between control and IL10(-/-) mice. Syndecan-4 was present on epithelial cells of all mice but was significantly reduced in IL10(-/-) mice. Differences in the expression of HS epitopes between control and IL10(-/-) mice were also confirmed. CONCLUSIONS: The study has revealed altered expression of syndecan-1 and -4 and HS epitopes in the gut of mice with an IBD-like gut disorder. The IL10(-/-) mouse is a useful model for further study of the functional role of HSPGs in chronic inflammation and in maintaining healthy gut barrier. (Inflamm Bowel Dis 2011;).
Original languageEnglish
Pages (from-to)1112-1126
Number of pages15
JournalInflammatory Bowel Diseases
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 2012

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Heparan Sulfate Proteoglycans
Colitis
Interleukin-10
Theoretical Models
Knockout Mice
Syndecans
Syndecan-4
Syndecan-1
Epitopes
Inflammatory Bowel Diseases
Syndecan-3
Mucous Membrane
Syndecan-2
Epithelial Cells
Messenger RNA
Heparitin Sulfate
Intestinal Mucosa
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Colon

Keywords

  • syndecan
  • heparan sulfate
  • interleukin 10
  • animal models
  • colon

Cite this

Patterson, A. M., Delday, M. I., van Kuppevelt, T. H., Loh, G., Blaut, M., Haller, D., ... Kelly, D. (2012). Expression of heparan sulfate proteoglycans in murine models of experimental colitis. Inflammatory Bowel Diseases, 18(6), 1112-1126. https://doi.org/10.1002/ibd.21879

Expression of heparan sulfate proteoglycans in murine models of experimental colitis. / Patterson, A M; Delday, M I; van Kuppevelt, T H; Loh, G; Blaut, M; Haller, D; Grant, G; Kelly, D.

In: Inflammatory Bowel Diseases, Vol. 18, No. 6, 06.2012, p. 1112-1126.

Research output: Contribution to journalArticle

Patterson, AM, Delday, MI, van Kuppevelt, TH, Loh, G, Blaut, M, Haller, D, Grant, G & Kelly, D 2012, 'Expression of heparan sulfate proteoglycans in murine models of experimental colitis', Inflammatory Bowel Diseases, vol. 18, no. 6, pp. 1112-1126. https://doi.org/10.1002/ibd.21879
Patterson, A M ; Delday, M I ; van Kuppevelt, T H ; Loh, G ; Blaut, M ; Haller, D ; Grant, G ; Kelly, D. / Expression of heparan sulfate proteoglycans in murine models of experimental colitis. In: Inflammatory Bowel Diseases. 2012 ; Vol. 18, No. 6. pp. 1112-1126.
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abstract = "BACKGROUND: Heparan sulfate proteoglycans (HSPGs) are considered important in maintaining physiological homeostasis in many systems. Their expression is altered greatly in several pathophysiological conditions. Herein, we assess the expression and cellular localization of HSPGs in two murine models of human inflammatory bowel disease (IBD). METHODS: Expression and localization of HSPGs, syndecans, and HS epitopes were examined in the colon of 129SvEv interleukin 10 knockout (IL10(-/-) ), C3Bir IL10(-/-) , and their genetic control (IL10(+/+) ) counterparts (129SvEv; C3H/HeJ). mRNA expression of syndecans and heparan sulfate biosynthesis enzymes were evaluated by real-time polymerase chain reaction (PCR). Localization of HSPGs was determined by immunofluorescence. RESULTS: mRNA for all syndecans was detected and expression in colonic tissues altered in IL10(-/-) mice. Syndecan-1 protein was expressed in the intestinal epithelium and on lamina propria cells of IL10(-/-) and control mice but was significantly reduced on the intestinal epithelial cells of IL10(-/-) , mice particularly with severe colitis. Syndecan-2 was not detected, whereas syndecan-3 immunoreactivity was localized in the lamina propria but did not differ between control and IL10(-/-) mice. Syndecan-4 was present on epithelial cells of all mice but was significantly reduced in IL10(-/-) mice. Differences in the expression of HS epitopes between control and IL10(-/-) mice were also confirmed. CONCLUSIONS: The study has revealed altered expression of syndecan-1 and -4 and HS epitopes in the gut of mice with an IBD-like gut disorder. The IL10(-/-) mouse is a useful model for further study of the functional role of HSPGs in chronic inflammation and in maintaining healthy gut barrier. (Inflamm Bowel Dis 2011;).",
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T1 - Expression of heparan sulfate proteoglycans in murine models of experimental colitis

AU - Patterson, A M

AU - Delday, M I

AU - van Kuppevelt, T H

AU - Loh, G

AU - Blaut, M

AU - Haller, D

AU - Grant, G

AU - Kelly, D

N1 - Copyright © 2011 Crohn's & Colitis Foundation of America, Inc.

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N2 - BACKGROUND: Heparan sulfate proteoglycans (HSPGs) are considered important in maintaining physiological homeostasis in many systems. Their expression is altered greatly in several pathophysiological conditions. Herein, we assess the expression and cellular localization of HSPGs in two murine models of human inflammatory bowel disease (IBD). METHODS: Expression and localization of HSPGs, syndecans, and HS epitopes were examined in the colon of 129SvEv interleukin 10 knockout (IL10(-/-) ), C3Bir IL10(-/-) , and their genetic control (IL10(+/+) ) counterparts (129SvEv; C3H/HeJ). mRNA expression of syndecans and heparan sulfate biosynthesis enzymes were evaluated by real-time polymerase chain reaction (PCR). Localization of HSPGs was determined by immunofluorescence. RESULTS: mRNA for all syndecans was detected and expression in colonic tissues altered in IL10(-/-) mice. Syndecan-1 protein was expressed in the intestinal epithelium and on lamina propria cells of IL10(-/-) and control mice but was significantly reduced on the intestinal epithelial cells of IL10(-/-) , mice particularly with severe colitis. Syndecan-2 was not detected, whereas syndecan-3 immunoreactivity was localized in the lamina propria but did not differ between control and IL10(-/-) mice. Syndecan-4 was present on epithelial cells of all mice but was significantly reduced in IL10(-/-) mice. Differences in the expression of HS epitopes between control and IL10(-/-) mice were also confirmed. CONCLUSIONS: The study has revealed altered expression of syndecan-1 and -4 and HS epitopes in the gut of mice with an IBD-like gut disorder. The IL10(-/-) mouse is a useful model for further study of the functional role of HSPGs in chronic inflammation and in maintaining healthy gut barrier. (Inflamm Bowel Dis 2011;).

AB - BACKGROUND: Heparan sulfate proteoglycans (HSPGs) are considered important in maintaining physiological homeostasis in many systems. Their expression is altered greatly in several pathophysiological conditions. Herein, we assess the expression and cellular localization of HSPGs in two murine models of human inflammatory bowel disease (IBD). METHODS: Expression and localization of HSPGs, syndecans, and HS epitopes were examined in the colon of 129SvEv interleukin 10 knockout (IL10(-/-) ), C3Bir IL10(-/-) , and their genetic control (IL10(+/+) ) counterparts (129SvEv; C3H/HeJ). mRNA expression of syndecans and heparan sulfate biosynthesis enzymes were evaluated by real-time polymerase chain reaction (PCR). Localization of HSPGs was determined by immunofluorescence. RESULTS: mRNA for all syndecans was detected and expression in colonic tissues altered in IL10(-/-) mice. Syndecan-1 protein was expressed in the intestinal epithelium and on lamina propria cells of IL10(-/-) and control mice but was significantly reduced on the intestinal epithelial cells of IL10(-/-) , mice particularly with severe colitis. Syndecan-2 was not detected, whereas syndecan-3 immunoreactivity was localized in the lamina propria but did not differ between control and IL10(-/-) mice. Syndecan-4 was present on epithelial cells of all mice but was significantly reduced in IL10(-/-) mice. Differences in the expression of HS epitopes between control and IL10(-/-) mice were also confirmed. CONCLUSIONS: The study has revealed altered expression of syndecan-1 and -4 and HS epitopes in the gut of mice with an IBD-like gut disorder. The IL10(-/-) mouse is a useful model for further study of the functional role of HSPGs in chronic inflammation and in maintaining healthy gut barrier. (Inflamm Bowel Dis 2011;).

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