Expression of MAX2 under SCARECROW promoter enhances the strigolactone/MAX2 dependent response of Arabidopsis roots to low-phosphate conditions

Ortal Madmon, Moran Mazuz, Puja Kumari, Anandamoy Dam, Aurel Ion, Einav Mayzlish-Gati, Eduard Belausov, Smadar Wininger, Mohamad Abu-Abied, Christopher S.P. McErlean, Liam J. Bromhead, Rafael Perl-Treves, Cristina Prandi, Yoram Kapulnik, Hinanit Koltai*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Main conclusion: MAX2/strigolactone signaling in the endodermis and/or quiescent center of the root is partiallysufficient to exert changes in F-actin density and cellular trafficking in the root epidermis, and alter gene expression during plant response to low Pi conditions. Strigolactones (SLs) are a new group of plant hormones that regulate different developmental processes in the plant via MAX2, an F-box protein that interacts with their receptor. SLs and MAX2 are necessary for the marked increase in root-hair (RH) density in seedlings under conditions of phosphate (Pi) deprivation. This marked elevation was associated with an active reduction in actin-filament density and endosomal movement in root epidermal cells. Also, expression of MAX2 under the SCARECROW (SCR) promoter was sufficient to confer SL sensitivity in roots, suggesting that SL signaling pathways act through a root-specific, yet non-cell-autonomous regulatory mode of action. Here we show evidence for a non-cell autonomous signaling of SL/MAX2, originating from the root endodermis, and necessary for seedling response to conditions of Pi deprivation. SCR-derived expression of MAX2 in max2-1 mutant background promoted the root low Pi response, whereas supplementation of the synthetic SL GR24 to these SCR:MAX2 expressing lines further enhanced this response. Moreover, the SCR:MAX2 expression led to changes in actin density and endosome movement in epidermal cells and in TIR1 and PHO2 gene expression. These results demonstrate that MAX2 signaling in the endodermis and/or quiescent center is partially sufficient to exert changes in F-actin density and cellular trafficking in the epidermis, and alter gene expression under low Pi conditions.

Original languageEnglish
Pages (from-to)1419-1427
Number of pages9
JournalPlanta
Volume243
Issue number6
Early online date26 Feb 2016
DOIs
Publication statusPublished - 1 Jun 2016

Keywords

  • MAX2
  • Phosphate
  • Phosphate-starvation induced genes
  • Root
  • Root hair
  • SCARECROW
  • Strigolactones

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