Expression of one-hybrid fusions with Staphylococcus aureus lexA in Candida albicans confirms that Nrg1 is a transcriptional repressor and that Gcn4 is a transcriptional activator

Claire L. Russell, Alistair J. P. Brown

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In the pathogenic fungus, Candida albicans, Nrgl down-regulates the expression of morphogenetic genes and is presumed to act as a transcriptional repressor. In contrast, Gcn4 up-regulates amino acid biosynthetic genes and is presumed to be a transcriptional activator. However, these presumptions remain to be tested directly. A classic approach has been to use a one-hybrid assay that exploits the Escherichia coli lexA protein fusions. However in C albicans, the alternate decoding of CUG as serine prevents the expression of heterologous genes such as lexA, which contain numerous CUG codons. Therefore, we have developed a one-hybrid system, based on the Staphylococcus aureus lexA gene, as a tool for one-hybrid analyses of transcription factors in C. dalbicans. Using this one-hybrid system we have confirmed directly the positive and negative transcriptional activities of Nrgl and Gcn4 in C. albicans. (c) 2005 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)676-683
Number of pages7
JournalFungal Genetics and Biology
Volume42
Issue number8
Early online date8 Jun 2005
DOIs
Publication statusPublished - Aug 2005

Keywords

  • Candida albicans
  • transcription
  • Gcn4
  • Nrg1
  • lexA3
  • protein-protein interactions
  • regulates hyphal development
  • amino-acid starvation
  • saccharomyces-cerevisiae
  • filamentous growth
  • gene-expression
  • morphogenesis
  • virulence
  • yeast
  • system

Cite this

Expression of one-hybrid fusions with Staphylococcus aureus lexA in Candida albicans confirms that Nrg1 is a transcriptional repressor and that Gcn4 is a transcriptional activator. / Russell, Claire L.; Brown, Alistair J. P.

In: Fungal Genetics and Biology, Vol. 42, No. 8, 08.2005, p. 676-683.

Research output: Contribution to journalArticle

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abstract = "In the pathogenic fungus, Candida albicans, Nrgl down-regulates the expression of morphogenetic genes and is presumed to act as a transcriptional repressor. In contrast, Gcn4 up-regulates amino acid biosynthetic genes and is presumed to be a transcriptional activator. However, these presumptions remain to be tested directly. A classic approach has been to use a one-hybrid assay that exploits the Escherichia coli lexA protein fusions. However in C albicans, the alternate decoding of CUG as serine prevents the expression of heterologous genes such as lexA, which contain numerous CUG codons. Therefore, we have developed a one-hybrid system, based on the Staphylococcus aureus lexA gene, as a tool for one-hybrid analyses of transcription factors in C. dalbicans. Using this one-hybrid system we have confirmed directly the positive and negative transcriptional activities of Nrgl and Gcn4 in C. albicans. (c) 2005 Elsevier Inc. All rights reserved.",
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