Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions

D. M. Reid, M. Montoya, P. R. Taylor, P. Borrow, S. Gordon, G. D. Brown, Simon Yuk Chun Wong

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Dectin-1 is a pathogen-re cognition receptor on macrophages (MPhis), neutrophils, and dendritic cells (DCs). On MPhis and bone marrow-derived DCs, it has been shown to mediate the nonopsonic recognition of and response to soluble and particulate yeast beta-glucans. We have optimized the immunohistochemical detection of Dectin-1 and demonstrated its expression on neutrophils, subpopulations of MPhis in splenic red and white pulp, alveolar MPhis, Kupffer cells, and MPhis and DCs in the lamina propria of gut villi. This is consistent with its role in pathogen surveillance. A significant proportion of CD11c(+) splenic DCs expressed Dectin-1, but expression was not restricted to any one subset. Dectin-1 expression was low on resident MPhis and DCs of skin and was not detected on resident MPhis or DCs in kidney, heart, brain, or eye. The proposed, additional role of Dectin-1 as a coreceptor for T cell activation is supported by its expression on DCs in the T cell areas of the spleen and lymph nodes. Strong expression of Dectin-1 on subpopulations of MPhis and DCs in the medullary and corticomedullary regions of the thymus suggests a role distinct from pathogen recognition. Tissue localization thus revealed potential roles of Dectin-1 in leukocyte interactions during innate immune responses and T cell development.

Original languageEnglish
Pages (from-to)86-94
Number of pages8
JournalJournal of Leukocyte Biology
Volume76
DOIs
Publication statusPublished - 2004

Keywords

  • innate immunity
  • macrophages
  • dendritic cells
  • spleen
  • thymus development
  • MARGINAL ZONE MACROPHAGES
  • SPLEEN DENDRITIC CELLS
  • MANNOSE RECEPTOR
  • DIFFERENTIATION ANTIGENS
  • CD8 EXPRESSION
  • MOUSE
  • VIVO
  • LIPOPOLYSACCHARIDE
  • SUBPOPULATION
  • HETEROGENEITY

Cite this

Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions. / Reid, D. M.; Montoya, M.; Taylor, P. R.; Borrow, P.; Gordon, S.; Brown, G. D.; Wong, Simon Yuk Chun.

In: Journal of Leukocyte Biology, Vol. 76, 2004, p. 86-94.

Research output: Contribution to journalArticle

Reid, D. M. ; Montoya, M. ; Taylor, P. R. ; Borrow, P. ; Gordon, S. ; Brown, G. D. ; Wong, Simon Yuk Chun. / Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions. In: Journal of Leukocyte Biology. 2004 ; Vol. 76. pp. 86-94.
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abstract = "Dectin-1 is a pathogen-re cognition receptor on macrophages (MPhis), neutrophils, and dendritic cells (DCs). On MPhis and bone marrow-derived DCs, it has been shown to mediate the nonopsonic recognition of and response to soluble and particulate yeast beta-glucans. We have optimized the immunohistochemical detection of Dectin-1 and demonstrated its expression on neutrophils, subpopulations of MPhis in splenic red and white pulp, alveolar MPhis, Kupffer cells, and MPhis and DCs in the lamina propria of gut villi. This is consistent with its role in pathogen surveillance. A significant proportion of CD11c(+) splenic DCs expressed Dectin-1, but expression was not restricted to any one subset. Dectin-1 expression was low on resident MPhis and DCs of skin and was not detected on resident MPhis or DCs in kidney, heart, brain, or eye. The proposed, additional role of Dectin-1 as a coreceptor for T cell activation is supported by its expression on DCs in the T cell areas of the spleen and lymph nodes. Strong expression of Dectin-1 on subpopulations of MPhis and DCs in the medullary and corticomedullary regions of the thymus suggests a role distinct from pathogen recognition. Tissue localization thus revealed potential roles of Dectin-1 in leukocyte interactions during innate immune responses and T cell development.",
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AU - Reid, D. M.

AU - Montoya, M.

AU - Taylor, P. R.

AU - Borrow, P.

AU - Gordon, S.

AU - Brown, G. D.

AU - Wong, Simon Yuk Chun

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AB - Dectin-1 is a pathogen-re cognition receptor on macrophages (MPhis), neutrophils, and dendritic cells (DCs). On MPhis and bone marrow-derived DCs, it has been shown to mediate the nonopsonic recognition of and response to soluble and particulate yeast beta-glucans. We have optimized the immunohistochemical detection of Dectin-1 and demonstrated its expression on neutrophils, subpopulations of MPhis in splenic red and white pulp, alveolar MPhis, Kupffer cells, and MPhis and DCs in the lamina propria of gut villi. This is consistent with its role in pathogen surveillance. A significant proportion of CD11c(+) splenic DCs expressed Dectin-1, but expression was not restricted to any one subset. Dectin-1 expression was low on resident MPhis and DCs of skin and was not detected on resident MPhis or DCs in kidney, heart, brain, or eye. The proposed, additional role of Dectin-1 as a coreceptor for T cell activation is supported by its expression on DCs in the T cell areas of the spleen and lymph nodes. Strong expression of Dectin-1 on subpopulations of MPhis and DCs in the medullary and corticomedullary regions of the thymus suggests a role distinct from pathogen recognition. Tissue localization thus revealed potential roles of Dectin-1 in leukocyte interactions during innate immune responses and T cell development.

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KW - dendritic cells

KW - spleen

KW - thymus development

KW - MARGINAL ZONE MACROPHAGES

KW - SPLEEN DENDRITIC CELLS

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KW - DIFFERENTIATION ANTIGENS

KW - CD8 EXPRESSION

KW - MOUSE

KW - VIVO

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