Expression of the p53 tumour suppressor gene product is a determinant of chemosensitivity

R D Petty, I A Cree, L A Sutherland, E M Hunter, D P Lane, P E Preece, P E Andreotti, Russell David Petty

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Abstract

Many cytotoxic agents act by causing DNA damage, and the p53 tumour suppressor gene is known to be involved in the cellular response to DNA damage. Since inactivation of p53 is common in many tumours, we wondered if this would affect the sensitivity of cancer cells to cytotoxic agents. We have shown that this is indeed the case in transformed mouse cell lines with and without a mutated p53 gene; p53 "knockout" mouse fibroblasts, and normal human skin fibroblasts treated with an anti-sense p53 oligonucleotide. In addition, we have demonstrated a correlation between p53 protein expression in human breast cancer specimens and their chemosensitivity. The results show that inactivation or mutation of p53 renders cells more sensitive to those cytotoxic drugs whose primary mechanism of action is DNA damage.

Original languageEnglish
Pages (from-to)264-70
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume199
Issue number1
Publication statusPublished - 28 Feb 1994

Keywords

  • Animals
  • Antibiotics, Antineoplastic
  • Base Sequence
  • Cell Line
  • Gene Expression
  • Genes, p53
  • Humans
  • In Vitro Techniques
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Oligonucleotides, Antisense
  • RNA, Messenger
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53

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    Petty, R. D., Cree, I. A., Sutherland, L. A., Hunter, E. M., Lane, D. P., Preece, P. E., Andreotti, P. E., & Petty, R. D. (1994). Expression of the p53 tumour suppressor gene product is a determinant of chemosensitivity. Biochemical and Biophysical Research Communications, 199(1), 264-70.