Factors influencing the clearance of a genetically attenuated (ΔaroA) strain of Aeromonas salmonicida from rainbow trout Oncorhynchus mykiss

M. J. Marsden, E. Collins, C. J. Secombes*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Clearance of an aroA deletion mutant of Aeromonas salmonicida from, tissues of rainbow trout Oncorhynchus mykiss was studied under a number of conditions. Following intraperitoneal injection into trout, the bacteria were rapidly found in all tissues/body fluids examined (kidney, spleen, liver, heart, muscle, blood and peritoneal fluid), with highest recoveries in the spleen and peritoneal fluid Whilst in some tissues numbers increased over the first few days post-injection, in general clearance had been effected within 7 to 9 d at 16°C regardless of the inoculum, dose. However, with the highest dose (2 × 109 bacteria per fish) used some fish mortalities occurred. Temperature was shown to influence clearance, with low water temperatures significantly delaying clearance. Whole body homogenates confirmed that some increase in bactenal numbers occurred in vivo, after an initial and immediate dramatic drop in bacterial viability. Finally, a 2 h crowding stress which significantly elevated blood glucose levels had no effect upon clearance. These results are discussed with respect to the possible use of this deletion mutant as a live vaccine in aquaculture.

Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalDiseases of Aquatic Organisms
Volume27
Issue number2
DOIs
Publication statusPublished - 14 Nov 1996

Keywords

  • Aeromonas salmonicida
  • Clearance
  • Furunculosis
  • Genetically attenuated vaccine
  • Rainbow trout
  • ΔAroA

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