Factors influencing the expression of interleukin-1 beta in cultured rainbow trout (Oncorhynchus mykiss) leucocytes

J Zou, J Holland, O Pleguezuelos, C Cunningham, C J Secombes

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115 Citations (Scopus)

Abstract

The dose dependency and kinetics of lipopolysaccharide (LPS) induced interleukin-1 beta (IL-1 beta) mRNA expression in rainbow trout leucocytes has been studied. Northern blot analysis revealed weak hybridisation of a trout IL-1 beta probe to RNA from head kidney leucocytes stimulated with 0.1 mu g LPS/ml, with a large increase in IL-1 beta transcript level seen between 0.1 and 1.5 mu g LPS/ml. Using 5 mu g LPS/ml expression was first detectable 1-2 h post-stimulation. By 4 h post-stimulation maximal induction was seen but by 24-48 h the level had fallen and by 72 h no transcript was detectable. Culture temperature had a marked effect on IL-1 beta expression, with low temperatures inhibiting transcription. Indeed, an eight-fold increase in IL-1 beta transcript level was seen between cells cultured at 4 degrees C and 22 degrees C. Preincubation with cortisol was also shown to inhibit LPS-induced IL-1 beta expression. (C) 2000 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)575-582
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume24
Issue number6-7
Early online date19 May 2000
DOIs
Publication statusPublished - Sep 2000

Keywords

  • IL-1 beta
  • rainbow trout
  • expression
  • RT-PCR
  • Northern blot
  • temperature
  • cortisol
  • immune system
  • fish
  • protein
  • cloning
  • gene

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