Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay

Vololoniaina Raharinosy, Jean Michel Heraud* (Corresponding Author), Harinirina Aina Rabemananjara, Sandra Telfer, Danielle Aurore Doll Rakoto, Claudia Filippone, Jean Marc Reynes

*Corresponding author for this work

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Abstract

Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.

Original languageEnglish
Article number718
Number of pages7
JournalViruses
Volume11
Issue number8
DOIs
Publication statusPublished - 6 Aug 2019

Keywords

  • Hantavirus
  • Real-time RT-PCR
  • Thailand orthohantavirus

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