Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay

Vololoniaina Raharinosy; Jean Michel Heraud; Harinirina Aina Rabemananjara; Sandra Telfer; Danielle Aurore Doll Rakoto; Claudia Filippone; Jean Marc Reynes

doi:10.3390/v11080718

Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay

Vololoniaina Raharinosy, Jean Michel Heraud^* (Corresponding Author), Harinirina Aina Rabemananjara, Sandra Telfer, Danielle Aurore Doll Rakoto, Claudia Filippone, Jean Marc Reynes

^*Corresponding author for this work

Aberdeen Centre For Environmental Sustainability

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Abstract

Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.

Original language	English
Article number	718
Number of pages	7
Journal	Viruses
Volume	11
Issue number	8
DOIs	https://doi.org/10.3390/v11080718
Publication status	Published - 6 Aug 2019

Bibliographical note

Funding: This study was supported by the Programme Transversal de Recherche (PTR 505) funded by the Institut Pasteur International Network. V.R. was also supported though Girard’s fellowship undergraduate program from the Institut Pasteur de Madagascar and traineeship grants Calmette and Yersin program from the Institut Pasteur International Network.
Acknowledgements: We would like to express our gratitude to the staff of the Plague Central Laboratory Unit, Institut Pasteur de Madagascar: Minoarisoa Rajerison, Fehivola Mandanirina Andriamiarimanana, and Soanandrasana Rahelinirina for conducting the field work and for providing samples

Keywords

Hantavirus
Real-time RT-PCR
Thailand orthohantavirus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay. / Raharinosy, Vololoniaina; Heraud, Jean Michel (Corresponding Author); Rabemananjara, Harinirina Aina et al.
In: Viruses, Vol. 11, No. 8, 718, 06.08.2019.

Research output: Contribution to journal › Article › peer-review

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abstract = "Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.",

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AU - Filippone, Claudia

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Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay

Abstract

Bibliographical note

Keywords

UN SDGs

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