Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay

Vololoniaina Raharinosy, Jean Michel Heraud* (Corresponding Author), Harinirina Aina Rabemananjara, Sandra Telfer, Danielle Aurore Doll Rakoto, Claudia Filippone, Jean Marc Reynes

*Corresponding author for this work

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Abstract

Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.

Original languageEnglish
Article number718
Number of pages7
JournalViruses
Volume11
Issue number8
DOIs
Publication statusPublished - 6 Aug 2019

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Thailand
Reverse Transcription
Indian Ocean
Polymerase Chain Reaction
Far East
Islands
Real-Time Polymerase Chain Reaction
Rodentia

Keywords

  • Hantavirus
  • Real-time RT-PCR
  • Thailand orthohantavirus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay. / Raharinosy, Vololoniaina; Heraud, Jean Michel (Corresponding Author); Rabemananjara, Harinirina Aina; Telfer, Sandra; Rakoto, Danielle Aurore Doll; Filippone, Claudia; Reynes, Jean Marc.

In: Viruses, Vol. 11, No. 8, 718, 06.08.2019.

Research output: Contribution to journalArticle

Raharinosy, Vololoniaina ; Heraud, Jean Michel ; Rabemananjara, Harinirina Aina ; Telfer, Sandra ; Rakoto, Danielle Aurore Doll ; Filippone, Claudia ; Reynes, Jean Marc. / Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay. In: Viruses. 2019 ; Vol. 11, No. 8.
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