Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay

Vololoniaina Raharinosy; Jean Michel Heraud; Harinirina Aina Rabemananjara; Sandra Telfer; Danielle Aurore Doll Rakoto; Claudia Filippone; Jean Marc Reynes

doi:10.3390/v11080718

Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay

Vololoniaina Raharinosy, Jean Michel Heraud^* (Corresponding Author), Harinirina Aina Rabemananjara, Sandra Telfer, Danielle Aurore Doll Rakoto, Claudia Filippone, Jean Marc Reynes

^*Corresponding author for this work

Aberdeen Centre For Environmental Sustainability

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Abstract

Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.

Original language	English
Article number	718
Number of pages	7
Journal	Viruses
Volume	11
Issue number	8
DOIs	https://doi.org/10.3390/v11080718
Publication status	Published - 6 Aug 2019

Keywords

Hantavirus
Real-time RT-PCR
Thailand orthohantavirus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay. / Raharinosy, Vololoniaina; Heraud, Jean Michel (Corresponding Author); Rabemananjara, Harinirina Aina et al.

In: Viruses, Vol. 11, No. 8, 718, 06.08.2019.

Research output: Contribution to journal › Article › peer-review

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abstract = "Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.",

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author = "Vololoniaina Raharinosy and Heraud, {Jean Michel} and Rabemananjara, {Harinirina Aina} and Sandra Telfer and Rakoto, {Danielle Aurore Doll} and Claudia Filippone and Reynes, {Jean Marc}",

note = "Funding: This study was supported by the Programme Transversal de Recherche (PTR 505) funded by the Institut Pasteur International Network. V.R. was also supported though Girard{\textquoteright}s fellowship undergraduate program from the Institut Pasteur de Madagascar and traineeship grants Calmette and Yersin program from the Institut Pasteur International Network. Acknowledgements: We would like to express our gratitude to the staff of the Plague Central Laboratory Unit, Institut Pasteur de Madagascar: Minoarisoa Rajerison, Fehivola Mandanirina Andriamiarimanana, and Soanandrasana Rahelinirina for conducting the field work and for providing samples",

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AU - Rabemananjara, Harinirina Aina

AU - Telfer, Sandra

AU - Rakoto, Danielle Aurore Doll

AU - Filippone, Claudia

AU - Reynes, Jean Marc

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KW - Real-time RT-PCR

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Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay

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Keywords

UN SDGs

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