Fatty acid metabolism in human preimplantation embryos

Paul Haggarty, Maureen Jessie Wood, Elizabeth Ferguson, Gwen Hoad, Arasaratnam Srikantharajah, Eric Milne, Margaret Elizabeth Hamilton, Siladitya Bhattacharya

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

BACKGROUND: Little is known of fatty acid metabolism in human embryos. This information would be useful in developing metabolic tests of embryo quality and improving embryo culture media. METHODS: The fatty acid composition of human embryos and their ability to accumulate C-13 labelled fatty acids was assessed in relation to the stage of development using gas-chromatography and combustion-isotope-ratio-mass spectrometry. RESULTS: Compared with embryos which did not develop beyond the 4-cell stage, those that did had significantly higher concentrations of the unsaturates, linoleic (12% versus 3%; P = 0.02) and oleic (14% versus 7%; P = 0.02), and a lower concentration of total saturates (62% versus 77%; P = 0.04). There was uptake of both C-13 linoleic and palmitic, but the developmental pattern was different for each fatty acid. The net accumulation in pmol/embryo/24h for palmitic was 1 at the 2-cell to < 8-cell stage, 4 at the 8-cell-morula stage and negligible at the blastocyst stage. For linoleic, there was little net accumulation at the 2-cell to < 8-cell stage, 8 (8-cell-morula stage) and 17 pmol/embryo/24h (blastocyst stage). CONCLUSION: Preimplantation human embryos actively take up individual fatty acids at different rates at different stages of development. The high unsaturated concentration at the later stages of development may be explained by preferential uptake of linoleic acid.

Original languageEnglish
Pages (from-to)766-773
Number of pages7
JournalHuman Reproduction
Volume21
Issue number3
DOIs
Publication statusPublished - 2006

Keywords

  • embryo
  • fatty acids
  • IVF
  • linoleic acid
  • stem cells
  • in-vitro fertilization
  • protein-kinase-C
  • bovine oocytes
  • body-fat
  • turnover
  • immature

Cite this

Haggarty, P., Wood, M. J., Ferguson, E., Hoad, G., Srikantharajah, A., Milne, E., ... Bhattacharya, S. (2006). Fatty acid metabolism in human preimplantation embryos. Human Reproduction, 21(3), 766-773. https://doi.org/10.1093/humrep/dei385

Fatty acid metabolism in human preimplantation embryos. / Haggarty, Paul; Wood, Maureen Jessie; Ferguson, Elizabeth; Hoad, Gwen; Srikantharajah, Arasaratnam; Milne, Eric; Hamilton, Margaret Elizabeth; Bhattacharya, Siladitya.

In: Human Reproduction, Vol. 21, No. 3, 2006, p. 766-773.

Research output: Contribution to journalArticle

Haggarty, P, Wood, MJ, Ferguson, E, Hoad, G, Srikantharajah, A, Milne, E, Hamilton, ME & Bhattacharya, S 2006, 'Fatty acid metabolism in human preimplantation embryos', Human Reproduction, vol. 21, no. 3, pp. 766-773. https://doi.org/10.1093/humrep/dei385
Haggarty P, Wood MJ, Ferguson E, Hoad G, Srikantharajah A, Milne E et al. Fatty acid metabolism in human preimplantation embryos. Human Reproduction. 2006;21(3):766-773. https://doi.org/10.1093/humrep/dei385
Haggarty, Paul ; Wood, Maureen Jessie ; Ferguson, Elizabeth ; Hoad, Gwen ; Srikantharajah, Arasaratnam ; Milne, Eric ; Hamilton, Margaret Elizabeth ; Bhattacharya, Siladitya. / Fatty acid metabolism in human preimplantation embryos. In: Human Reproduction. 2006 ; Vol. 21, No. 3. pp. 766-773.
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abstract = "BACKGROUND: Little is known of fatty acid metabolism in human embryos. This information would be useful in developing metabolic tests of embryo quality and improving embryo culture media. METHODS: The fatty acid composition of human embryos and their ability to accumulate C-13 labelled fatty acids was assessed in relation to the stage of development using gas-chromatography and combustion-isotope-ratio-mass spectrometry. RESULTS: Compared with embryos which did not develop beyond the 4-cell stage, those that did had significantly higher concentrations of the unsaturates, linoleic (12{\%} versus 3{\%}; P = 0.02) and oleic (14{\%} versus 7{\%}; P = 0.02), and a lower concentration of total saturates (62{\%} versus 77{\%}; P = 0.04). There was uptake of both C-13 linoleic and palmitic, but the developmental pattern was different for each fatty acid. The net accumulation in pmol/embryo/24h for palmitic was 1 at the 2-cell to < 8-cell stage, 4 at the 8-cell-morula stage and negligible at the blastocyst stage. For linoleic, there was little net accumulation at the 2-cell to < 8-cell stage, 8 (8-cell-morula stage) and 17 pmol/embryo/24h (blastocyst stage). CONCLUSION: Preimplantation human embryos actively take up individual fatty acids at different rates at different stages of development. The high unsaturated concentration at the later stages of development may be explained by preferential uptake of linoleic acid.",
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AU - Haggarty, Paul

AU - Wood, Maureen Jessie

AU - Ferguson, Elizabeth

AU - Hoad, Gwen

AU - Srikantharajah, Arasaratnam

AU - Milne, Eric

AU - Hamilton, Margaret Elizabeth

AU - Bhattacharya, Siladitya

PY - 2006

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N2 - BACKGROUND: Little is known of fatty acid metabolism in human embryos. This information would be useful in developing metabolic tests of embryo quality and improving embryo culture media. METHODS: The fatty acid composition of human embryos and their ability to accumulate C-13 labelled fatty acids was assessed in relation to the stage of development using gas-chromatography and combustion-isotope-ratio-mass spectrometry. RESULTS: Compared with embryos which did not develop beyond the 4-cell stage, those that did had significantly higher concentrations of the unsaturates, linoleic (12% versus 3%; P = 0.02) and oleic (14% versus 7%; P = 0.02), and a lower concentration of total saturates (62% versus 77%; P = 0.04). There was uptake of both C-13 linoleic and palmitic, but the developmental pattern was different for each fatty acid. The net accumulation in pmol/embryo/24h for palmitic was 1 at the 2-cell to < 8-cell stage, 4 at the 8-cell-morula stage and negligible at the blastocyst stage. For linoleic, there was little net accumulation at the 2-cell to < 8-cell stage, 8 (8-cell-morula stage) and 17 pmol/embryo/24h (blastocyst stage). CONCLUSION: Preimplantation human embryos actively take up individual fatty acids at different rates at different stages of development. The high unsaturated concentration at the later stages of development may be explained by preferential uptake of linoleic acid.

AB - BACKGROUND: Little is known of fatty acid metabolism in human embryos. This information would be useful in developing metabolic tests of embryo quality and improving embryo culture media. METHODS: The fatty acid composition of human embryos and their ability to accumulate C-13 labelled fatty acids was assessed in relation to the stage of development using gas-chromatography and combustion-isotope-ratio-mass spectrometry. RESULTS: Compared with embryos which did not develop beyond the 4-cell stage, those that did had significantly higher concentrations of the unsaturates, linoleic (12% versus 3%; P = 0.02) and oleic (14% versus 7%; P = 0.02), and a lower concentration of total saturates (62% versus 77%; P = 0.04). There was uptake of both C-13 linoleic and palmitic, but the developmental pattern was different for each fatty acid. The net accumulation in pmol/embryo/24h for palmitic was 1 at the 2-cell to < 8-cell stage, 4 at the 8-cell-morula stage and negligible at the blastocyst stage. For linoleic, there was little net accumulation at the 2-cell to < 8-cell stage, 8 (8-cell-morula stage) and 17 pmol/embryo/24h (blastocyst stage). CONCLUSION: Preimplantation human embryos actively take up individual fatty acids at different rates at different stages of development. The high unsaturated concentration at the later stages of development may be explained by preferential uptake of linoleic acid.

KW - embryo

KW - fatty acids

KW - IVF

KW - linoleic acid

KW - stem cells

KW - in-vitro fertilization

KW - protein-kinase-C

KW - bovine oocytes

KW - body-fat

KW - turnover

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