Feasibility of using prokaryote biosensors to assess acute toxicity of polycyclic aromatic hydrocarbons

BJ Reid, KT Semple*, CJ Macleod, HJ Weitz, GI Paton

*Corresponding author for this work

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The aim of this study was to assess the acute toxicity of polycyclic aromatic hydrocarbons using lux-marked bacterial biosensors, Standard solutions of phenanthrene, pyrene and benzo[a]pyrene were produced using 50 mM hydroxpropyl-beta-cyclodextrin solution which contained each respective polycyclic aromatic hydrocarbon at 6.25 times the aqueous solubility limit of the compound. The polycyclic aromatic hydrocarbon solutions were incubated with each of the biosensors for 280 min and the bioluminescence monitored every 20 min. Over the incubation time period, there was no significant decrease in bioluminescence in any of the biosensors tested with the exception of Rhizobium leguminosarum biovar trifolii TAl luxAB. In this series of incubations, there was a dramatic increase in bioluminescence in the presence of phenanthrene (2.5 times) and benzo[a]pyrene (3 times) above that of the background control (biosensor without polycyclic aromatic hydrocarbon) after 20 min. Over the next 3 h, bioluminescence decreased to that of the control. An ATP assay was carried out on the biosensors to assess if uncoupling of the oxidative phosphorylation mechanisms in the respiratory chain of the cells had occurred. However, it was found that the polycyclic aromatic hydrocarbons had no effect on the organisms indicating that there was no uncoupling. Additionally, mineralisation studies using C-14-labelled polycyclic aromatic hydrocarbons showed that the biosensors could not mineralise the compounds. This study has shown that the three polycyclic aromatic hydrocarbons tested are not acutely toxic to the prokaryotic biosensors tested, although acute toxicity has been shown in other bioassays. These results question the rationale for using prokaryote biosensors to assess the toxicity of hydrophobic chemicals, such as polycyclic aromatic hydrocarbons. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)227-233
Number of pages7
JournalFEMS Microbiology Letters
Volume169
Issue number2
DOIs
Publication statusPublished - 15 Dec 1998

Keywords

  • polycyclic aromatic hydrocarbon
  • acute toxicity
  • lux-marked bacteria
  • cyclodextrin
  • risk assessment
  • PSEUDOMONAS-FLUORESCENS
  • HEAVY-METALS
  • SOIL
  • BIOAVAILABILITY
  • BIODEGRADATION

Cite this

Feasibility of using prokaryote biosensors to assess acute toxicity of polycyclic aromatic hydrocarbons. / Reid, BJ; Semple, KT; Macleod, CJ; Weitz, HJ; Paton, GI.

In: FEMS Microbiology Letters, Vol. 169, No. 2, 15.12.1998, p. 227-233.

Research output: Contribution to journalArticle

Reid, BJ ; Semple, KT ; Macleod, CJ ; Weitz, HJ ; Paton, GI. / Feasibility of using prokaryote biosensors to assess acute toxicity of polycyclic aromatic hydrocarbons. In: FEMS Microbiology Letters. 1998 ; Vol. 169, No. 2. pp. 227-233.
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AB - The aim of this study was to assess the acute toxicity of polycyclic aromatic hydrocarbons using lux-marked bacterial biosensors, Standard solutions of phenanthrene, pyrene and benzo[a]pyrene were produced using 50 mM hydroxpropyl-beta-cyclodextrin solution which contained each respective polycyclic aromatic hydrocarbon at 6.25 times the aqueous solubility limit of the compound. The polycyclic aromatic hydrocarbon solutions were incubated with each of the biosensors for 280 min and the bioluminescence monitored every 20 min. Over the incubation time period, there was no significant decrease in bioluminescence in any of the biosensors tested with the exception of Rhizobium leguminosarum biovar trifolii TAl luxAB. In this series of incubations, there was a dramatic increase in bioluminescence in the presence of phenanthrene (2.5 times) and benzo[a]pyrene (3 times) above that of the background control (biosensor without polycyclic aromatic hydrocarbon) after 20 min. Over the next 3 h, bioluminescence decreased to that of the control. An ATP assay was carried out on the biosensors to assess if uncoupling of the oxidative phosphorylation mechanisms in the respiratory chain of the cells had occurred. However, it was found that the polycyclic aromatic hydrocarbons had no effect on the organisms indicating that there was no uncoupling. Additionally, mineralisation studies using C-14-labelled polycyclic aromatic hydrocarbons showed that the biosensors could not mineralise the compounds. This study has shown that the three polycyclic aromatic hydrocarbons tested are not acutely toxic to the prokaryotic biosensors tested, although acute toxicity has been shown in other bioassays. These results question the rationale for using prokaryote biosensors to assess the toxicity of hydrophobic chemicals, such as polycyclic aromatic hydrocarbons. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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KW - HEAVY-METALS

KW - SOIL

KW - BIOAVAILABILITY

KW - BIODEGRADATION

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