Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans

Meng Yang, Alexandra Brand, Thyagarajan Srikantha, Karla J. Daniels, David R. Soll, Neil A. R. Gow

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Few mating-regulated genes have been characterized in Candida albicans. C. albicans FIG1 (CaFIG1) is a fungus-specific and mating-induced gene encoding a putative 4-transmembrane domain protein that shares sequence similarities with members of the claudin superfamily. In Saccharomyces cerevisiae, Fig1 is required for shmoo fusion and is upregulated in response to mating pheromones. Expression of CaFIG1 was also strongly activated in the presence of cells of the opposite mating type. CaFig1-green fluorescent protein (GFP) was visible only during the mating response, when it localized predominantly to the plasma membrane and perinuclear zone in mating projections and daughter cells. At the plasma membrane, CaFig1-GFP was visualized as discontinuous zones, but the distribution of perinuclear CaFig1-GFP was homogeneous. Exposure to pheromone induced a 5-fold increase in Ca(2+) uptake in mating-competent opaque cells. Uptake was reduced substantially in the fig1¿ null mutant. CaFig1 is therefore involved in Ca(2+) influx and localizes to membranes that are destined to undergo fusion during mating.
Original languageEnglish
Pages (from-to)435-444
Number of pages10
JournalEukaryotic Cell
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 2011

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Green Fluorescent Proteins
Candida albicans
Pheromones
Calcium
Membranes
Cell Membrane
Genes
Saccharomyces cerevisiae
Fungi

Keywords

  • Amino Acid Sequence
  • Biological Transport
  • Calcium
  • Candida albicans
  • Cell Membrane
  • Fungal Proteins
  • Gene Expression Regulation, Fungal
  • Genes, Mating Type, Fungal
  • Molecular Sequence Data
  • Pheromones
  • Protein Transport
  • Sequence Alignment

Cite this

Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans. / Yang, Meng; Brand, Alexandra; Srikantha, Thyagarajan; Daniels, Karla J.; Soll, David R.; Gow, Neil A. R.

In: Eukaryotic Cell, Vol. 10, No. 3, 03.2011, p. 435-444.

Research output: Contribution to journalArticle

Yang, Meng ; Brand, Alexandra ; Srikantha, Thyagarajan ; Daniels, Karla J. ; Soll, David R. ; Gow, Neil A. R. / Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans. In: Eukaryotic Cell. 2011 ; Vol. 10, No. 3. pp. 435-444.
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abstract = "Few mating-regulated genes have been characterized in Candida albicans. C. albicans FIG1 (CaFIG1) is a fungus-specific and mating-induced gene encoding a putative 4-transmembrane domain protein that shares sequence similarities with members of the claudin superfamily. In Saccharomyces cerevisiae, Fig1 is required for shmoo fusion and is upregulated in response to mating pheromones. Expression of CaFIG1 was also strongly activated in the presence of cells of the opposite mating type. CaFig1-green fluorescent protein (GFP) was visible only during the mating response, when it localized predominantly to the plasma membrane and perinuclear zone in mating projections and daughter cells. At the plasma membrane, CaFig1-GFP was visualized as discontinuous zones, but the distribution of perinuclear CaFig1-GFP was homogeneous. Exposure to pheromone induced a 5-fold increase in Ca(2+) uptake in mating-competent opaque cells. Uptake was reduced substantially in the fig1¿ null mutant. CaFig1 is therefore involved in Ca(2+) influx and localizes to membranes that are destined to undergo fusion during mating.",
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