Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin

Ali Roghanian, Des C. Jones, Jogi V. Pattisapu, Jonathan Wolfe, Neil T. Young, Babak Behnam

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Testis-specific gene antigen 10 (TSGA10) encodes an 82-kDa protein expressed during development, and in testis and brain tissues. We report its expression in human monocyte-derived dendritic cells (DC) and macrophages in vitro and in murine spleen CD11c(+) cells ex vivo. An interaction between DC/macrophage-derived TSGA10 and vimentin, as well as a few other major cytoskeletal proteins (e.g., actin-¿1), was identified by pull-down and mass spectroscopy assays. The interaction between TSGA10 and vimentin was further confirmed by immunoprecipitation and immunolocalisation in transfected RAW267 and HEK293 cell lines. TSGA10 formed filamentous structures in transfected COS-1 cells and was observed in cellular projections. We propose that TSGA10 could influence the function of antigen presenting cells (APC) via its interaction with cytoskeletal proteins such as vimentin.
Original languageEnglish
Pages (from-to)120-126
Number of pages7
JournalCellular Immunology
Volume265
Issue number2
DOIs
Publication statusPublished - 2010

Fingerprint

Vimentin
Antigen-Presenting Cells
Testis
Antigens
Genes
Proteins
Cytoskeletal Proteins
Dendritic Cells
Macrophages
HEK293 Cells
COS Cells
Immunoprecipitation
Actins
Monocytes
Mass Spectrometry
Spleen
Cell Line
Brain

Keywords

  • actins
  • animals
  • antigens, CD11c
  • cell line
  • cytoskeleton
  • dendritic cells
  • humans
  • macrophages
  • male
  • mass spectrometry
  • mice
  • monocytes
  • protein binding
  • proteins
  • spleen
  • testis
  • vimentin

Cite this

Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin. / Roghanian, Ali; Jones, Des C.; Pattisapu, Jogi V.; Wolfe, Jonathan; Young, Neil T.; Behnam, Babak.

In: Cellular Immunology, Vol. 265, No. 2, 2010, p. 120-126.

Research output: Contribution to journalArticle

Roghanian, Ali ; Jones, Des C. ; Pattisapu, Jogi V. ; Wolfe, Jonathan ; Young, Neil T. ; Behnam, Babak. / Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin. In: Cellular Immunology. 2010 ; Vol. 265, No. 2. pp. 120-126.
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abstract = "Testis-specific gene antigen 10 (TSGA10) encodes an 82-kDa protein expressed during development, and in testis and brain tissues. We report its expression in human monocyte-derived dendritic cells (DC) and macrophages in vitro and in murine spleen CD11c(+) cells ex vivo. An interaction between DC/macrophage-derived TSGA10 and vimentin, as well as a few other major cytoskeletal proteins (e.g., actin-¿1), was identified by pull-down and mass spectroscopy assays. The interaction between TSGA10 and vimentin was further confirmed by immunoprecipitation and immunolocalisation in transfected RAW267 and HEK293 cell lines. TSGA10 formed filamentous structures in transfected COS-1 cells and was observed in cellular projections. We propose that TSGA10 could influence the function of antigen presenting cells (APC) via its interaction with cytoskeletal proteins such as vimentin.",
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T1 - Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin

AU - Roghanian, Ali

AU - Jones, Des C.

AU - Pattisapu, Jogi V.

AU - Wolfe, Jonathan

AU - Young, Neil T.

AU - Behnam, Babak

N1 - Copyright © 2010 Elsevier Inc. All rights reserved.

PY - 2010

Y1 - 2010

N2 - Testis-specific gene antigen 10 (TSGA10) encodes an 82-kDa protein expressed during development, and in testis and brain tissues. We report its expression in human monocyte-derived dendritic cells (DC) and macrophages in vitro and in murine spleen CD11c(+) cells ex vivo. An interaction between DC/macrophage-derived TSGA10 and vimentin, as well as a few other major cytoskeletal proteins (e.g., actin-¿1), was identified by pull-down and mass spectroscopy assays. The interaction between TSGA10 and vimentin was further confirmed by immunoprecipitation and immunolocalisation in transfected RAW267 and HEK293 cell lines. TSGA10 formed filamentous structures in transfected COS-1 cells and was observed in cellular projections. We propose that TSGA10 could influence the function of antigen presenting cells (APC) via its interaction with cytoskeletal proteins such as vimentin.

AB - Testis-specific gene antigen 10 (TSGA10) encodes an 82-kDa protein expressed during development, and in testis and brain tissues. We report its expression in human monocyte-derived dendritic cells (DC) and macrophages in vitro and in murine spleen CD11c(+) cells ex vivo. An interaction between DC/macrophage-derived TSGA10 and vimentin, as well as a few other major cytoskeletal proteins (e.g., actin-¿1), was identified by pull-down and mass spectroscopy assays. The interaction between TSGA10 and vimentin was further confirmed by immunoprecipitation and immunolocalisation in transfected RAW267 and HEK293 cell lines. TSGA10 formed filamentous structures in transfected COS-1 cells and was observed in cellular projections. We propose that TSGA10 could influence the function of antigen presenting cells (APC) via its interaction with cytoskeletal proteins such as vimentin.

KW - actins

KW - animals

KW - antigens, CD11c

KW - cell line

KW - cytoskeleton

KW - dendritic cells

KW - humans

KW - macrophages

KW - male

KW - mass spectrometry

KW - mice

KW - monocytes

KW - protein binding

KW - proteins

KW - spleen

KW - testis

KW - vimentin

U2 - 10.1016/j.cellimm.2010.07.012

DO - 10.1016/j.cellimm.2010.07.012

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EP - 126

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 2

ER -