Fish oil positively regulates anabolic signalling alongside an increase in whole-body gluconeogenesis in ageing skeletal muscle

Torkamol Kamolrat, Stuart R Gray, M. Carole Thivierge

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Purpose
Fish oil, containing mainly long-chain n-3 polyunsaturated fatty acids (LCn-3PUFA), has been found to acutely stimulate protein synthesis and insulin-mediated glucose metabolism. However, the underlying mechanism and more prolonged effect of fish oil during ageing remain to be determined.
Methods
Fish oil (EPAX6000; 49.6 % eicosapentaenoic acid, 50.4 % docosahexaenoic acid) or control oil (60 % olive, 40 % soy) supplementation was delivered, via chocolate-derived sweets, to rats for 8 weeks. Throughout the study, food intake and body weight were recorded and body composition was investigated using EchoMRI. During the last 40 min of a 6 h infusion, with labelled dextrose ([U-13C]glucose) and amino acids ([1-13C]phenylalanine), blood samples were collected to assess glucose and phenylalanine kinetics. Soleus and longissimus dorsi muscles were extracted for protein and mRNA analyses.
Results
Fish oil had no effect on food intake or body composition. An increased whole-body glucose turnover, mainly accounted for via an increase in endogenous glucose production, was observed with fish oil feeding. No effects on whole-body phenylalanine turnover were observed. In longissimus dorsi, fish oil augmented the phosphorylation of phosphoinositide 3-kinase (PI3K)[Tyr458] (P = 0.04) and 70 kDa ribosomal protein S6 kinase (p70s6k)[Thr389] (P = 0.04). There were no differences in protein kinase B (Akt)[Ser473], mammalian target of rapamycin (mTOR)[Ser2448], protein phosphatase 2A (PP2A) 56 kDa regulatory B subunit ¿ (PP2A-B56-¿), forkhead box containing proteins O-subclass 3a (FOX03a)[Ser253] or inflammatory markers (Interleukin-6, Interleukin-1 ß, tumour necrosis factor-a, and cyclooxygenase-2).
Conclusions
Our data suggest that the fish oil may stimulate endogenous glucose production and increase anabolic signalling in ageing rats.
Original languageEnglish
Pages (from-to)647-657
Number of pages11
JournalEuropean Journal of Nutrition
Volume52
Issue number2
Early online date13 May 2012
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Gluconeogenesis
Fish Oils
Skeletal Muscle
Glucose
Phenylalanine
70-kDa Ribosomal Protein S6 Kinases
Protein Phosphatase 2
Body Composition
Eating
TOR Serine-Threonine Kinases
Forkhead Transcription Factors
Proto-Oncogene Proteins c-akt
Eicosapentaenoic Acid
1-Phosphatidylinositol 4-Kinase
Docosahexaenoic Acids
Omega-3 Fatty Acids
Cyclooxygenase 2
Interleukin-1
Interleukin-6
Proteins

Keywords

  • ageing
  • fish oil
  • p70s6k
  • metabolism
  • insulin sensitivity

Cite this

Fish oil positively regulates anabolic signalling alongside an increase in whole-body gluconeogenesis in ageing skeletal muscle. / Kamolrat, Torkamol; Gray, Stuart R; Carole Thivierge, M.

In: European Journal of Nutrition, Vol. 52, No. 2, 03.2013, p. 647-657.

Research output: Contribution to journalArticle

Kamolrat, Torkamol ; Gray, Stuart R ; Carole Thivierge, M. / Fish oil positively regulates anabolic signalling alongside an increase in whole-body gluconeogenesis in ageing skeletal muscle. In: European Journal of Nutrition. 2013 ; Vol. 52, No. 2. pp. 647-657.
@article{4f0031697a304bd687fd717c9da51969,
title = "Fish oil positively regulates anabolic signalling alongside an increase in whole-body gluconeogenesis in ageing skeletal muscle",
abstract = "Purpose Fish oil, containing mainly long-chain n-3 polyunsaturated fatty acids (LCn-3PUFA), has been found to acutely stimulate protein synthesis and insulin-mediated glucose metabolism. However, the underlying mechanism and more prolonged effect of fish oil during ageing remain to be determined. Methods Fish oil (EPAX6000; 49.6 {\%} eicosapentaenoic acid, 50.4 {\%} docosahexaenoic acid) or control oil (60 {\%} olive, 40 {\%} soy) supplementation was delivered, via chocolate-derived sweets, to rats for 8 weeks. Throughout the study, food intake and body weight were recorded and body composition was investigated using EchoMRI. During the last 40 min of a 6 h infusion, with labelled dextrose ([U-13C]glucose) and amino acids ([1-13C]phenylalanine), blood samples were collected to assess glucose and phenylalanine kinetics. Soleus and longissimus dorsi muscles were extracted for protein and mRNA analyses. Results Fish oil had no effect on food intake or body composition. An increased whole-body glucose turnover, mainly accounted for via an increase in endogenous glucose production, was observed with fish oil feeding. No effects on whole-body phenylalanine turnover were observed. In longissimus dorsi, fish oil augmented the phosphorylation of phosphoinositide 3-kinase (PI3K)[Tyr458] (P = 0.04) and 70 kDa ribosomal protein S6 kinase (p70s6k)[Thr389] (P = 0.04). There were no differences in protein kinase B (Akt)[Ser473], mammalian target of rapamycin (mTOR)[Ser2448], protein phosphatase 2A (PP2A) 56 kDa regulatory B subunit ¿ (PP2A-B56-¿), forkhead box containing proteins O-subclass 3a (FOX03a)[Ser253] or inflammatory markers (Interleukin-6, Interleukin-1 {\ss}, tumour necrosis factor-a, and cyclooxygenase-2). Conclusions Our data suggest that the fish oil may stimulate endogenous glucose production and increase anabolic signalling in ageing rats.",
keywords = "ageing, fish oil, p70s6k, metabolism, insulin sensitivity",
author = "Torkamol Kamolrat and Gray, {Stuart R} and {Carole Thivierge}, M.",
year = "2013",
month = "3",
doi = "10.1007/s00394-012-0368-7",
language = "English",
volume = "52",
pages = "647--657",
journal = "European Journal of Nutrition",
issn = "1436-6207",
publisher = "D. Steinkopff-Verlag",
number = "2",

}

TY - JOUR

T1 - Fish oil positively regulates anabolic signalling alongside an increase in whole-body gluconeogenesis in ageing skeletal muscle

AU - Kamolrat, Torkamol

AU - Gray, Stuart R

AU - Carole Thivierge, M.

PY - 2013/3

Y1 - 2013/3

N2 - Purpose Fish oil, containing mainly long-chain n-3 polyunsaturated fatty acids (LCn-3PUFA), has been found to acutely stimulate protein synthesis and insulin-mediated glucose metabolism. However, the underlying mechanism and more prolonged effect of fish oil during ageing remain to be determined. Methods Fish oil (EPAX6000; 49.6 % eicosapentaenoic acid, 50.4 % docosahexaenoic acid) or control oil (60 % olive, 40 % soy) supplementation was delivered, via chocolate-derived sweets, to rats for 8 weeks. Throughout the study, food intake and body weight were recorded and body composition was investigated using EchoMRI. During the last 40 min of a 6 h infusion, with labelled dextrose ([U-13C]glucose) and amino acids ([1-13C]phenylalanine), blood samples were collected to assess glucose and phenylalanine kinetics. Soleus and longissimus dorsi muscles were extracted for protein and mRNA analyses. Results Fish oil had no effect on food intake or body composition. An increased whole-body glucose turnover, mainly accounted for via an increase in endogenous glucose production, was observed with fish oil feeding. No effects on whole-body phenylalanine turnover were observed. In longissimus dorsi, fish oil augmented the phosphorylation of phosphoinositide 3-kinase (PI3K)[Tyr458] (P = 0.04) and 70 kDa ribosomal protein S6 kinase (p70s6k)[Thr389] (P = 0.04). There were no differences in protein kinase B (Akt)[Ser473], mammalian target of rapamycin (mTOR)[Ser2448], protein phosphatase 2A (PP2A) 56 kDa regulatory B subunit ¿ (PP2A-B56-¿), forkhead box containing proteins O-subclass 3a (FOX03a)[Ser253] or inflammatory markers (Interleukin-6, Interleukin-1 ß, tumour necrosis factor-a, and cyclooxygenase-2). Conclusions Our data suggest that the fish oil may stimulate endogenous glucose production and increase anabolic signalling in ageing rats.

AB - Purpose Fish oil, containing mainly long-chain n-3 polyunsaturated fatty acids (LCn-3PUFA), has been found to acutely stimulate protein synthesis and insulin-mediated glucose metabolism. However, the underlying mechanism and more prolonged effect of fish oil during ageing remain to be determined. Methods Fish oil (EPAX6000; 49.6 % eicosapentaenoic acid, 50.4 % docosahexaenoic acid) or control oil (60 % olive, 40 % soy) supplementation was delivered, via chocolate-derived sweets, to rats for 8 weeks. Throughout the study, food intake and body weight were recorded and body composition was investigated using EchoMRI. During the last 40 min of a 6 h infusion, with labelled dextrose ([U-13C]glucose) and amino acids ([1-13C]phenylalanine), blood samples were collected to assess glucose and phenylalanine kinetics. Soleus and longissimus dorsi muscles were extracted for protein and mRNA analyses. Results Fish oil had no effect on food intake or body composition. An increased whole-body glucose turnover, mainly accounted for via an increase in endogenous glucose production, was observed with fish oil feeding. No effects on whole-body phenylalanine turnover were observed. In longissimus dorsi, fish oil augmented the phosphorylation of phosphoinositide 3-kinase (PI3K)[Tyr458] (P = 0.04) and 70 kDa ribosomal protein S6 kinase (p70s6k)[Thr389] (P = 0.04). There were no differences in protein kinase B (Akt)[Ser473], mammalian target of rapamycin (mTOR)[Ser2448], protein phosphatase 2A (PP2A) 56 kDa regulatory B subunit ¿ (PP2A-B56-¿), forkhead box containing proteins O-subclass 3a (FOX03a)[Ser253] or inflammatory markers (Interleukin-6, Interleukin-1 ß, tumour necrosis factor-a, and cyclooxygenase-2). Conclusions Our data suggest that the fish oil may stimulate endogenous glucose production and increase anabolic signalling in ageing rats.

KW - ageing

KW - fish oil

KW - p70s6k

KW - metabolism

KW - insulin sensitivity

U2 - 10.1007/s00394-012-0368-7

DO - 10.1007/s00394-012-0368-7

M3 - Article

VL - 52

SP - 647

EP - 657

JO - European Journal of Nutrition

JF - European Journal of Nutrition

SN - 1436-6207

IS - 2

ER -