Fish oil supplementation of the diet partly reverses the age-related increase in plasma soluble vascular cell adhesion molecule-1 (sVCAM-1) concentrations

E. A. Miles, Frank Thies, F. A. Wallace, J. R. Powell, T. L. Hurst, E. A. Newsholme, P. C. Calder

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Soluble forms of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin (termed sICAM-1, sVCAM-1 and sE-selectin respectively) are found in the plasma, and are elevated during inflammatory conditions in which there is increased expression of the cellular forms of the molecules on endothelial and other cells. sICAM-1, sVCAM-1 and sE-selectin concentrations were measured in the plasma of 140 healthy Caucasian subjects aged between 18 and 75 years (100 males/40 females). sICAM-1 concentrations varied between 59.9 and 299.7 ng/ml (median 150 ng/ml), sVCAM-1 concentrations varied between 222.8 and 1672.9 ng/ml (median 662 ng/ml) and sE-selectin concentrations varied between 12.4 and 90.3 ng/ml (median 45.5 ng/ml). There were significant positive linear correlations between age and the plasma concentrations of sICAM-1 (r = 0.580; P < 0.001) and sVCAM-1 (r = 0.392; P < 0.001), which were retained when the effects of gender, body mass index and fasting plasma triacylglycerol and total cholesterol concentrations were controlled for. The significant positive linear correlation between age and the plasma concentration of sE-selectin (r = 0.234; P = 0.027) was lost when other variables were controlled for. Male subjects < 40 years of age had significantly lower plasma concentrations of both sICAM-1 and sVCAM-1 than males > 55 years of age (both P < 0.001), but the difference in plasma sE-selectin concentrations between the age groups did not reach significance (P = 0.073). Subgroups of 16 males aged < 40 years and 12 elderly subjects (> 55 years of age) participated in a doubled-blind, placebo-controlled study of fish oil supplementation over 12 weeks. The level of eicosapentaenoic acid in plasma phospholipids did not change with placebo supplementation, but was significantly increased with fish oil supplementation in both young male and elderly subjects (median increase 200%). sICAM-1, sVCAM-1 and sE-selectin concentrations were unaffected by supplementation with placebo in either young male or elderly subjects, sICAM-1 concentrations were unaffected by fish oil supplementation. sE-selectin concentrations were significantly increased by fish oil supplementation in young males (P = 0.043; median increase 38%), but fish oil tended to decrease plasma sE-selectin concentrations in the elderly subjects (P = 0.075), with a median decrease of 11%. sVCAM-1 concentrations were unaffected by fish oil supplementation in young males. Fish oil supplementation significantly decreased plasma sVCAM-1 concentrations in the elderly subjects (P = 0.043), with a med lan decrease of 20% (range 16-60%). These observations suggest that fish oil decreases endothelial activation in elderly subjects.

Original languageEnglish
Pages (from-to)91-100
Number of pages9
JournalClinical Science
Volume100
DOIs
Publication statusPublished - 2001

Keywords

  • adhesion molecules
  • fish oil
  • n-3 fatty acids
  • POLYUNSATURATED FATTY-ACIDS
  • CORONARY HEART-DISEASE
  • HUMAN ATHEROSCLEROTIC LESIONS
  • ENDOTHELIAL-CELL-ADHESION
  • APPARENTLY HEALTHY-MEN
  • NF-KAPPA-B
  • E-SELECTIN
  • CAROTID ATHEROSCLEROSIS
  • TRANSCRIPTION FACTOR
  • LIPID-PEROXIDATION

Cite this

Fish oil supplementation of the diet partly reverses the age-related increase in plasma soluble vascular cell adhesion molecule-1 (sVCAM-1) concentrations. / Miles, E. A.; Thies, Frank; Wallace, F. A.; Powell, J. R.; Hurst, T. L.; Newsholme, E. A.; Calder, P. C.

In: Clinical Science, Vol. 100, 2001, p. 91-100.

Research output: Contribution to journalArticle

Miles, E. A. ; Thies, Frank ; Wallace, F. A. ; Powell, J. R. ; Hurst, T. L. ; Newsholme, E. A. ; Calder, P. C. / Fish oil supplementation of the diet partly reverses the age-related increase in plasma soluble vascular cell adhesion molecule-1 (sVCAM-1) concentrations. In: Clinical Science. 2001 ; Vol. 100. pp. 91-100.
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abstract = "Soluble forms of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin (termed sICAM-1, sVCAM-1 and sE-selectin respectively) are found in the plasma, and are elevated during inflammatory conditions in which there is increased expression of the cellular forms of the molecules on endothelial and other cells. sICAM-1, sVCAM-1 and sE-selectin concentrations were measured in the plasma of 140 healthy Caucasian subjects aged between 18 and 75 years (100 males/40 females). sICAM-1 concentrations varied between 59.9 and 299.7 ng/ml (median 150 ng/ml), sVCAM-1 concentrations varied between 222.8 and 1672.9 ng/ml (median 662 ng/ml) and sE-selectin concentrations varied between 12.4 and 90.3 ng/ml (median 45.5 ng/ml). There were significant positive linear correlations between age and the plasma concentrations of sICAM-1 (r = 0.580; P < 0.001) and sVCAM-1 (r = 0.392; P < 0.001), which were retained when the effects of gender, body mass index and fasting plasma triacylglycerol and total cholesterol concentrations were controlled for. The significant positive linear correlation between age and the plasma concentration of sE-selectin (r = 0.234; P = 0.027) was lost when other variables were controlled for. Male subjects < 40 years of age had significantly lower plasma concentrations of both sICAM-1 and sVCAM-1 than males > 55 years of age (both P < 0.001), but the difference in plasma sE-selectin concentrations between the age groups did not reach significance (P = 0.073). Subgroups of 16 males aged < 40 years and 12 elderly subjects (> 55 years of age) participated in a doubled-blind, placebo-controlled study of fish oil supplementation over 12 weeks. The level of eicosapentaenoic acid in plasma phospholipids did not change with placebo supplementation, but was significantly increased with fish oil supplementation in both young male and elderly subjects (median increase 200{\%}). sICAM-1, sVCAM-1 and sE-selectin concentrations were unaffected by supplementation with placebo in either young male or elderly subjects, sICAM-1 concentrations were unaffected by fish oil supplementation. sE-selectin concentrations were significantly increased by fish oil supplementation in young males (P = 0.043; median increase 38{\%}), but fish oil tended to decrease plasma sE-selectin concentrations in the elderly subjects (P = 0.075), with a median decrease of 11{\%}. sVCAM-1 concentrations were unaffected by fish oil supplementation in young males. Fish oil supplementation significantly decreased plasma sVCAM-1 concentrations in the elderly subjects (P = 0.043), with a med lan decrease of 20{\%} (range 16-60{\%}). These observations suggest that fish oil decreases endothelial activation in elderly subjects.",
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TY - JOUR

T1 - Fish oil supplementation of the diet partly reverses the age-related increase in plasma soluble vascular cell adhesion molecule-1 (sVCAM-1) concentrations

AU - Miles, E. A.

AU - Thies, Frank

AU - Wallace, F. A.

AU - Powell, J. R.

AU - Hurst, T. L.

AU - Newsholme, E. A.

AU - Calder, P. C.

PY - 2001

Y1 - 2001

N2 - Soluble forms of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin (termed sICAM-1, sVCAM-1 and sE-selectin respectively) are found in the plasma, and are elevated during inflammatory conditions in which there is increased expression of the cellular forms of the molecules on endothelial and other cells. sICAM-1, sVCAM-1 and sE-selectin concentrations were measured in the plasma of 140 healthy Caucasian subjects aged between 18 and 75 years (100 males/40 females). sICAM-1 concentrations varied between 59.9 and 299.7 ng/ml (median 150 ng/ml), sVCAM-1 concentrations varied between 222.8 and 1672.9 ng/ml (median 662 ng/ml) and sE-selectin concentrations varied between 12.4 and 90.3 ng/ml (median 45.5 ng/ml). There were significant positive linear correlations between age and the plasma concentrations of sICAM-1 (r = 0.580; P < 0.001) and sVCAM-1 (r = 0.392; P < 0.001), which were retained when the effects of gender, body mass index and fasting plasma triacylglycerol and total cholesterol concentrations were controlled for. The significant positive linear correlation between age and the plasma concentration of sE-selectin (r = 0.234; P = 0.027) was lost when other variables were controlled for. Male subjects < 40 years of age had significantly lower plasma concentrations of both sICAM-1 and sVCAM-1 than males > 55 years of age (both P < 0.001), but the difference in plasma sE-selectin concentrations between the age groups did not reach significance (P = 0.073). Subgroups of 16 males aged < 40 years and 12 elderly subjects (> 55 years of age) participated in a doubled-blind, placebo-controlled study of fish oil supplementation over 12 weeks. The level of eicosapentaenoic acid in plasma phospholipids did not change with placebo supplementation, but was significantly increased with fish oil supplementation in both young male and elderly subjects (median increase 200%). sICAM-1, sVCAM-1 and sE-selectin concentrations were unaffected by supplementation with placebo in either young male or elderly subjects, sICAM-1 concentrations were unaffected by fish oil supplementation. sE-selectin concentrations were significantly increased by fish oil supplementation in young males (P = 0.043; median increase 38%), but fish oil tended to decrease plasma sE-selectin concentrations in the elderly subjects (P = 0.075), with a median decrease of 11%. sVCAM-1 concentrations were unaffected by fish oil supplementation in young males. Fish oil supplementation significantly decreased plasma sVCAM-1 concentrations in the elderly subjects (P = 0.043), with a med lan decrease of 20% (range 16-60%). These observations suggest that fish oil decreases endothelial activation in elderly subjects.

AB - Soluble forms of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin (termed sICAM-1, sVCAM-1 and sE-selectin respectively) are found in the plasma, and are elevated during inflammatory conditions in which there is increased expression of the cellular forms of the molecules on endothelial and other cells. sICAM-1, sVCAM-1 and sE-selectin concentrations were measured in the plasma of 140 healthy Caucasian subjects aged between 18 and 75 years (100 males/40 females). sICAM-1 concentrations varied between 59.9 and 299.7 ng/ml (median 150 ng/ml), sVCAM-1 concentrations varied between 222.8 and 1672.9 ng/ml (median 662 ng/ml) and sE-selectin concentrations varied between 12.4 and 90.3 ng/ml (median 45.5 ng/ml). There were significant positive linear correlations between age and the plasma concentrations of sICAM-1 (r = 0.580; P < 0.001) and sVCAM-1 (r = 0.392; P < 0.001), which were retained when the effects of gender, body mass index and fasting plasma triacylglycerol and total cholesterol concentrations were controlled for. The significant positive linear correlation between age and the plasma concentration of sE-selectin (r = 0.234; P = 0.027) was lost when other variables were controlled for. Male subjects < 40 years of age had significantly lower plasma concentrations of both sICAM-1 and sVCAM-1 than males > 55 years of age (both P < 0.001), but the difference in plasma sE-selectin concentrations between the age groups did not reach significance (P = 0.073). Subgroups of 16 males aged < 40 years and 12 elderly subjects (> 55 years of age) participated in a doubled-blind, placebo-controlled study of fish oil supplementation over 12 weeks. The level of eicosapentaenoic acid in plasma phospholipids did not change with placebo supplementation, but was significantly increased with fish oil supplementation in both young male and elderly subjects (median increase 200%). sICAM-1, sVCAM-1 and sE-selectin concentrations were unaffected by supplementation with placebo in either young male or elderly subjects, sICAM-1 concentrations were unaffected by fish oil supplementation. sE-selectin concentrations were significantly increased by fish oil supplementation in young males (P = 0.043; median increase 38%), but fish oil tended to decrease plasma sE-selectin concentrations in the elderly subjects (P = 0.075), with a median decrease of 11%. sVCAM-1 concentrations were unaffected by fish oil supplementation in young males. Fish oil supplementation significantly decreased plasma sVCAM-1 concentrations in the elderly subjects (P = 0.043), with a med lan decrease of 20% (range 16-60%). These observations suggest that fish oil decreases endothelial activation in elderly subjects.

KW - adhesion molecules

KW - fish oil

KW - n-3 fatty acids

KW - POLYUNSATURATED FATTY-ACIDS

KW - CORONARY HEART-DISEASE

KW - HUMAN ATHEROSCLEROTIC LESIONS

KW - ENDOTHELIAL-CELL-ADHESION

KW - APPARENTLY HEALTHY-MEN

KW - NF-KAPPA-B

KW - E-SELECTIN

KW - CAROTID ATHEROSCLEROSIS

KW - TRANSCRIPTION FACTOR

KW - LIPID-PEROXIDATION

U2 - 10.1042/CS20000198

DO - 10.1042/CS20000198

M3 - Article

VL - 100

SP - 91

EP - 100

JO - Clinical Science

JF - Clinical Science

SN - 0143-5221

ER -