Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis

Yasushi Saka, T Sutani, Y Yamashita, S Saitoh, M Takeuchi, Y Nakaseko, M Yanagida

Research output: Contribution to journalArticle

272 Citations (Scopus)

Abstract

Fission yeast temperature-sensitive mutants cut3-477 and cut14-208 fail to condense chromosomes but small portions of the chromosomes can separate along the spindle during mitosis, producing phi-shaped chromosomes. Septation and cell division occur in the absence of normal nuclear division, causing the cut phenotype. Fluorescence in situ hybridization demonstrated that the contraction of the chromosome arm during mitosis was defective. Mutant chromosomes are apparently not rigid enough to be transported poleward by the spindle. Loss of the cut3 protein by gene disruption fails to maintain the nuclear chromatin architecture even in interphase. Both cut3 and cut14 proteins contain a putative nucleoside triphosphate (NTP)-binding domain and belong to the same ubiquitous protein family which includes the budding yeast Smc1 protein. The cut3 mutant was suppressed by an increase in the cut14+ gene dosage. The cut3 protein, having the highest similarity to the mouse protein, is localized in the nucleus throughout the cell cycle. Plasmids carrying the DNA topoisomerase I gene partly suppressed the temperature sensitive phenotype of cut3-477, suggesting that the cut3 protein might be involved in chromosome DNA topology.
Original languageEnglish
Pages (from-to)4938-4952
Number of pages15
JournalEMBO Journal
Volume13
Issue number20
Publication statusPublished - 17 Oct 1994

Fingerprint

Chromosome Segregation
Schizosaccharomyces
Chromosomes
Mitosis
Yeast
Condensation
Proteins
Genes
Cells
Cell Nucleus Division
Phenotype
Type I DNA Topoisomerase
Saccharomycetales
Temperature
Fungal Proteins
Gene Dosage
Interphase
Fluorescence In Situ Hybridization
Nucleosides
Cell Division

Keywords

  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins
  • Base Sequence
  • Cell Nucleus
  • Chromosomes, Fungal
  • DNA Topoisomerases, Type II
  • Fungal Proteins
  • Mice
  • Mitosis
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Sequence Homology, Amino Acid

Cite this

Saka, Y., Sutani, T., Yamashita, Y., Saitoh, S., Takeuchi, M., Nakaseko, Y., & Yanagida, M. (1994). Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis. EMBO Journal, 13(20), 4938-4952.

Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis. / Saka, Yasushi; Sutani, T; Yamashita, Y; Saitoh, S; Takeuchi, M; Nakaseko, Y; Yanagida, M.

In: EMBO Journal, Vol. 13, No. 20, 17.10.1994, p. 4938-4952.

Research output: Contribution to journalArticle

Saka, Y, Sutani, T, Yamashita, Y, Saitoh, S, Takeuchi, M, Nakaseko, Y & Yanagida, M 1994, 'Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis', EMBO Journal, vol. 13, no. 20, pp. 4938-4952.
Saka Y, Sutani T, Yamashita Y, Saitoh S, Takeuchi M, Nakaseko Y et al. Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis. EMBO Journal. 1994 Oct 17;13(20):4938-4952.
Saka, Yasushi ; Sutani, T ; Yamashita, Y ; Saitoh, S ; Takeuchi, M ; Nakaseko, Y ; Yanagida, M. / Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis. In: EMBO Journal. 1994 ; Vol. 13, No. 20. pp. 4938-4952.
@article{f53b9c747d434420a60c6fa395d2405e,
title = "Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis",
abstract = "Fission yeast temperature-sensitive mutants cut3-477 and cut14-208 fail to condense chromosomes but small portions of the chromosomes can separate along the spindle during mitosis, producing phi-shaped chromosomes. Septation and cell division occur in the absence of normal nuclear division, causing the cut phenotype. Fluorescence in situ hybridization demonstrated that the contraction of the chromosome arm during mitosis was defective. Mutant chromosomes are apparently not rigid enough to be transported poleward by the spindle. Loss of the cut3 protein by gene disruption fails to maintain the nuclear chromatin architecture even in interphase. Both cut3 and cut14 proteins contain a putative nucleoside triphosphate (NTP)-binding domain and belong to the same ubiquitous protein family which includes the budding yeast Smc1 protein. The cut3 mutant was suppressed by an increase in the cut14+ gene dosage. The cut3 protein, having the highest similarity to the mouse protein, is localized in the nucleus throughout the cell cycle. Plasmids carrying the DNA topoisomerase I gene partly suppressed the temperature sensitive phenotype of cut3-477, suggesting that the cut3 protein might be involved in chromosome DNA topology.",
keywords = "Amino Acid Sequence, Animals, Bacterial Proteins, Base Sequence, Cell Nucleus, Chromosomes, Fungal, DNA Topoisomerases, Type II, Fungal Proteins, Mice, Mitosis, Molecular Sequence Data, Mutation, Phenotype, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Homology, Amino Acid",
author = "Yasushi Saka and T Sutani and Y Yamashita and S Saitoh and M Takeuchi and Y Nakaseko and M Yanagida",
year = "1994",
month = "10",
day = "17",
language = "English",
volume = "13",
pages = "4938--4952",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "20",

}

TY - JOUR

T1 - Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis

AU - Saka, Yasushi

AU - Sutani, T

AU - Yamashita, Y

AU - Saitoh, S

AU - Takeuchi, M

AU - Nakaseko, Y

AU - Yanagida, M

PY - 1994/10/17

Y1 - 1994/10/17

N2 - Fission yeast temperature-sensitive mutants cut3-477 and cut14-208 fail to condense chromosomes but small portions of the chromosomes can separate along the spindle during mitosis, producing phi-shaped chromosomes. Septation and cell division occur in the absence of normal nuclear division, causing the cut phenotype. Fluorescence in situ hybridization demonstrated that the contraction of the chromosome arm during mitosis was defective. Mutant chromosomes are apparently not rigid enough to be transported poleward by the spindle. Loss of the cut3 protein by gene disruption fails to maintain the nuclear chromatin architecture even in interphase. Both cut3 and cut14 proteins contain a putative nucleoside triphosphate (NTP)-binding domain and belong to the same ubiquitous protein family which includes the budding yeast Smc1 protein. The cut3 mutant was suppressed by an increase in the cut14+ gene dosage. The cut3 protein, having the highest similarity to the mouse protein, is localized in the nucleus throughout the cell cycle. Plasmids carrying the DNA topoisomerase I gene partly suppressed the temperature sensitive phenotype of cut3-477, suggesting that the cut3 protein might be involved in chromosome DNA topology.

AB - Fission yeast temperature-sensitive mutants cut3-477 and cut14-208 fail to condense chromosomes but small portions of the chromosomes can separate along the spindle during mitosis, producing phi-shaped chromosomes. Septation and cell division occur in the absence of normal nuclear division, causing the cut phenotype. Fluorescence in situ hybridization demonstrated that the contraction of the chromosome arm during mitosis was defective. Mutant chromosomes are apparently not rigid enough to be transported poleward by the spindle. Loss of the cut3 protein by gene disruption fails to maintain the nuclear chromatin architecture even in interphase. Both cut3 and cut14 proteins contain a putative nucleoside triphosphate (NTP)-binding domain and belong to the same ubiquitous protein family which includes the budding yeast Smc1 protein. The cut3 mutant was suppressed by an increase in the cut14+ gene dosage. The cut3 protein, having the highest similarity to the mouse protein, is localized in the nucleus throughout the cell cycle. Plasmids carrying the DNA topoisomerase I gene partly suppressed the temperature sensitive phenotype of cut3-477, suggesting that the cut3 protein might be involved in chromosome DNA topology.

KW - Amino Acid Sequence

KW - Animals

KW - Bacterial Proteins

KW - Base Sequence

KW - Cell Nucleus

KW - Chromosomes, Fungal

KW - DNA Topoisomerases, Type II

KW - Fungal Proteins

KW - Mice

KW - Mitosis

KW - Molecular Sequence Data

KW - Mutation

KW - Phenotype

KW - Schizosaccharomyces

KW - Schizosaccharomyces pombe Proteins

KW - Sequence Homology, Amino Acid

M3 - Article

VL - 13

SP - 4938

EP - 4952

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 20

ER -