Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control

Yasushi Saka, P Fantes, T Sutani, C McInerny, J Creanor, M Yanagida

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Fission yeast temperature-sensitive cut5 (cell untimely torn) mutants are defective in initiation and/or elongation of DNA replication but allow mitosis and cell division at a restrictive temperature. We show that the cut5 protein (identical to rad4) (i) is an essential component of the replication checkpoint system but not the DNA damage checkpoint, and (ii) negatively regulates the activation of M phase kinase at mitotic entry. Even if the replication checkpoint has been activated previously, cut5 mutations allow mitosis and cell division after shift to 36 degrees C. Transcription of cut5+ is not under the control of the START gene cdc10+. The cut5 protein is enriched in the nucleus, consisting of repeating domains. An essential domain which resembles the proto-oncoprotein Ect2 has a strong negative effect on the entry into mitosis when overexpressed. Expression of the cut5 mutant phenotype requires the function of the M phase regulator genes cdc2+, cdc25+ and cdc13+. The cut5 protein forms a novel, essential link between DNA synthesis and M phase activation in the replication checkpoint control pathway.
Original languageEnglish
Pages (from-to)5319-5329
Number of pages11
JournalEMBO Journal
Volume13
Issue number22
Publication statusPublished - 15 Nov 1994

Fingerprint

Schizosaccharomyces
Cell Division
Yeast
Chromatin
Mitosis
DNA
Genes
Chemical activation
Cells
Proteins
Oncogene Proteins
Transcription
Elongation
Phosphotransferases
Temperature
Regulator Genes
DNA Replication
DNA Damage
Phenotype
Mutation

Keywords

  • Amino Acid Sequence
  • Chromatids
  • Chromatin
  • Chromosomes, Fungal
  • Consensus Sequence
  • DNA Replication
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Gene Expression Regulation, Fungal
  • Metaphase
  • Molecular Sequence Data
  • Proto-Oncogene Proteins
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Transglutaminases

Cite this

Saka, Y., Fantes, P., Sutani, T., McInerny, C., Creanor, J., & Yanagida, M. (1994). Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control. EMBO Journal, 13(22), 5319-5329.

Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control. / Saka, Yasushi; Fantes, P; Sutani, T; McInerny, C; Creanor, J; Yanagida, M.

In: EMBO Journal, Vol. 13, No. 22, 15.11.1994, p. 5319-5329.

Research output: Contribution to journalArticle

Saka, Y, Fantes, P, Sutani, T, McInerny, C, Creanor, J & Yanagida, M 1994, 'Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control' EMBO Journal, vol. 13, no. 22, pp. 5319-5329.
Saka Y, Fantes P, Sutani T, McInerny C, Creanor J, Yanagida M. Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control. EMBO Journal. 1994 Nov 15;13(22):5319-5329.
Saka, Yasushi ; Fantes, P ; Sutani, T ; McInerny, C ; Creanor, J ; Yanagida, M. / Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control. In: EMBO Journal. 1994 ; Vol. 13, No. 22. pp. 5319-5329.
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