Five subfamilies of β-defensin genes are present in salmonids: evolutionary insights and expression analysis in Atlantic salmon Salmo salar

Anna Harte, Guangming Tian, Qiaoqing Xu, Christopher John Secombes* (Corresponding Author), Tiehui Wang (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

β-defensins (BD) are the largest family of vertebrate defensins with potent antimicrobial, chemotactic and immune-regulatory activities. Four BD genes (BD1-4) have been cloned previously in rainbow trout but none have been reported in other salmonids. In this study seven BD genes (BD1a-b, 2–4, 5a-b) are characterised in Atlantic salmon and additional BD genes (BD1b and BD5) in rainbow trout. Bioinformatic analysis revealed up to seven BD genes in the genomes of other salmonids that belong to five subfamilies (BD1-5) due to whole genome duplications. BD1-2 and BD4-5 are also present in basal teleosts but only BD1 and/or BD5 are present in advanced teleosts due to loss of one chromosomal locus. BD3 is salmonid specific. Fish BD have a unique three-coding exon structure. Fish BD are highly divergent between subfamilies but conserved within each subfamily. Atlantic salmon BD genes are differentially expressed in tissues, often with low level expression in systemic immune organs (head kidney and spleen) yet with at least one BD gene highly expressed in mucosal tissues, heart, blood and liver. This suggests an important role of these BD genes in innate immunity in mucosa, liver and blood in Atlantic salmon.
Original languageEnglish
Article number103560
Number of pages15
JournalDevelopmental and Comparative Immunology
Volume104
Early online date20 Nov 2019
DOIs
Publication statusPublished - Mar 2020

Keywords

  • Atlantic salmon
  • β-defensin
  • expression
  • synteny
  • phylogenetic tree
  • evolution
  • CYTOKINES
  • Phylogenetic tree
  • Synteny
  • FUNCTIONAL-CHARACTERIZATION
  • IDENTIFICATION
  • FAMILY
  • beta-defensin
  • PEPTIDES
  • PATTERN
  • Expression
  • Evolution
  • HOMOLOGS
  • TROUT ONCORHYNCHUS-MYKISS
  • IMMUNE DEFENSE
  • MODULATION

Fingerprint Dive into the research topics of 'Five subfamilies of β-defensin genes are present in salmonids: evolutionary insights and expression analysis in Atlantic salmon <i>Salmo salar</i>'. Together they form a unique fingerprint.

Cite this