Fully-automated radiosynthesis and in vitro uptake investigation of [N-methyl-¹¹C]methylene blue

Lutz F Schweiger, Tim A D Smith

Research output: Contribution to journalArticle

Abstract

Malignant melanoma is a type of skin cancer which can spread rapidly if not detected early and left untreated. Positron Emission Tomography (PET) is a powerful imaging technique for detecting cancer but with only a limited number of radiotracers available the development of novel PET probes for detection and prevention of cancer is imperative. In the present study we present the fully-automated radiosynthesis of [N-methyl-(11)C]methylene blue and an in vitro uptake study in metastasic melanoma cell lines. Using the GE TRACERlab FXc Pro module [N-methyl-(11)C]methylene blue was isolated via solid-phase extraction in an average time of 36 min after end of bombardment and formulated with a radiochemical purity greater than 95%. The in vitro uptake study of [N-methyl-(11)C]methylene blue in SK-MEL28 melanin-expressing melanoma cell line demonstrated in site-specific binding of 51% promoting it as a promising melanoma PET imaging agent.

Original languageEnglish
Pages (from-to)4267-4270
Number of pages4
JournalAnticancer Research
Volume33
Issue number10
Publication statusPublished - Oct 2013

Keywords

  • Automation, Laboratory
  • Carbon Radioisotopes
  • Cell Line, Tumor
  • Chemistry Techniques, Synthetic
  • Humans
  • Methylene Blue
  • Radiopharmaceuticals
  • [N-methyl-11C]methylene blue
  • PET
  • TRACERlab FXc PRO module
  • Melanoma

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