Functional analysis of the phospholipase C gene CaPLC1 and two unusual phospholipase C genes, CaPLC2 and CaPLC3, of Candida albicans

Donika Kunze, Inga Melzer, Desiree Bennett, Dominique Sanglard, Donna Margaret MacCallum, Jan Nörskau, David C Coleman, Frank Christopher Odds, Wilhelm Schäfer, Bernhard Hube

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Phospholipases C are known to be important regulators of cellular processes but may also act as virulence factors of pathogenic microbes. At least three genes in the genome of the human-pathogenic fungus Candida albicans encode phospholipases with conserved phospholipase C (Plc) motifs. None of the deduced protein sequences contain N-terminal signal peptides, suggesting that these phospholipases are not secreted. In contrast to its orthologue in Sacharomyces cerevisiae, CaPLC1 seems to be an essential gene. However, a conditional mutant with reduced transcript levels of CaPLC1 had phenotypes similar to Plc1p-deficient mutants in S. cerevisiae, including reduced growth on media causing increased osmotic stress, on media with a non-glucose carbon source, or at elevated or lower temperatures, suggesting that CaPlc1p, like the Plc1p counterpart in S. cerevisiae, may be involved in multiple cellular processes. Furthermore, phenotypic screening of the heterozygous DeltaCaplc1/CaPLC1 mutant showed additional defects in hyphal formation. The loss of CaPLC1 cannot be compensated by two additional PLC genes of C. albicans (CaPLC2 and CaPLC3) encoding two almost identical phospholipases C with no counterpart in S. cerevisiae but containing structural elements found in bacterial phospholipases C. Although the promoter sequences of CaPLC2 and CaPLC3 differed dramatically, the transcriptional pattern of both genes was similar. In contrast to CaPLC1, CaPLC2 and CaPLC3 are not essential. Although Caplc2/3 mutants had reduced abilities to produce hyphae on solid media, these mutants were as virulent as the wild-type in a model of systemic infection. These data suggest that C. albicans contains two different classes of phospholipases C which are involved in cellular processes but which have no specific functions in pathogenicity.
Original languageEnglish
Pages (from-to)3381-94
Number of pages14
JournalMicrobiology
Volume151
Issue number10
DOIs
Publication statusPublished - 1 Oct 2005

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Type C Phospholipases
Candida albicans
Saccharomyces cerevisiae
Phospholipases
Genes
Hyphae
Essential Genes
Osmotic Pressure
Virulence Factors
Human Genome
Protein Sorting Signals
Virulence
Fungi
Carbon
Phenotype
Temperature
Growth
Infection
Proteins

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Candida albicans
  • Candidiasis
  • Drosophila Proteins
  • Fungal Proteins
  • Gene Expression Profiling
  • Gene Expression Regulation, Fungal
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • Protein-Serine-Threonine Kinases
  • Sequence Analysis, DNA
  • Transcription, Genetic
  • Type C Phospholipases

Cite this

Functional analysis of the phospholipase C gene CaPLC1 and two unusual phospholipase C genes, CaPLC2 and CaPLC3, of Candida albicans. / Kunze, Donika; Melzer, Inga; Bennett, Desiree; Sanglard, Dominique; MacCallum, Donna Margaret; Nörskau, Jan; Coleman, David C; Odds, Frank Christopher; Schäfer, Wilhelm; Hube, Bernhard.

In: Microbiology , Vol. 151, No. 10, 01.10.2005, p. 3381-94.

Research output: Contribution to journalArticle

Kunze, D, Melzer, I, Bennett, D, Sanglard, D, MacCallum, DM, Nörskau, J, Coleman, DC, Odds, FC, Schäfer, W & Hube, B 2005, 'Functional analysis of the phospholipase C gene CaPLC1 and two unusual phospholipase C genes, CaPLC2 and CaPLC3, of Candida albicans', Microbiology , vol. 151, no. 10, pp. 3381-94. https://doi.org/10.1099/mic.0.28353-0
Kunze, Donika ; Melzer, Inga ; Bennett, Desiree ; Sanglard, Dominique ; MacCallum, Donna Margaret ; Nörskau, Jan ; Coleman, David C ; Odds, Frank Christopher ; Schäfer, Wilhelm ; Hube, Bernhard. / Functional analysis of the phospholipase C gene CaPLC1 and two unusual phospholipase C genes, CaPLC2 and CaPLC3, of Candida albicans. In: Microbiology . 2005 ; Vol. 151, No. 10. pp. 3381-94.
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AU - MacCallum, Donna Margaret

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KW - Mice

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KW - Phenotype

KW - Protein-Serine-Threonine Kinases

KW - Sequence Analysis, DNA

KW - Transcription, Genetic

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