Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus)

Zhitao Qi*, Shisi Ren, Qihuan Zhang, Jun Zou, Qiaoqing Xu, Zisheng Wang, Guo Qiao, Pin Nie, Mingxian Chang

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)
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Abstract

Peptidoglycan (PGN) recognition proteins (PGRPs) are important pattern recognition receptors (PRRs) involved in immune defense against bacterial infections. In this study, a short PGRP (termed AdPGRP-S1) was cloned and functionally characterized from Chinese giant salamander (Andrias davidianus), the largest extant urodela amphibian species. AdPGRP-S1 was 184 aa in length and shared 38.7%- 54.9% sequence identities with other vertebrates' short PGRPs. It contained one typical PGRP domain at the C-terminal region and several conserved amino acid (aa) residues involved in amidase and PGN binding. AdPGRP-S1 was constitutively expressed in all tissues examined, with the highest expression level seen in spleen and intestine. It has been shown that AdPGRP-S1 could bind and degrade Lys-PGN and Dap-PGN. Further, AdPGRP-S1 had antibacterial activity against the Gram-negative bacteria, Edwardsiella tarda, and was able to trigger the activation of NF-κB signaling. These results demonstrated that AdPGRP-S1 possesses multiple functions in pathogen recognition, mediating ceullular signaling, and initiating antibacterial response. This is the first functional study of a salamander PGRP, providing insight to further understand the functional evolution of verterbates' PGRPs.

Original languageEnglish
Pages (from-to)99323-99335
Number of pages13
JournalOncotarget
Volume8
Issue number59
DOIs
Publication statusPublished - 3 Oct 2017

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Keywords

  • Andrias davidianus
  • Functional analysis
  • Gene clone
  • Immune response
  • Immunity
  • Immunology and Microbiology Section
  • Peptidoglycan recognition protein

ASJC Scopus subject areas

  • Oncology

Cite this

Qi, Z., Ren, S., Zhang, Q., Zou, J., Xu, Q., Wang, Z., Qiao, G., Nie, P., & Chang, M. (2017). Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus). Oncotarget, 8(59), 99323-99335. https://doi.org/10.18632/oncotarget.21470