Functional Magnetic Resonance Imaging for In Vivo Quantification of Pulmonary Hypertension in the Sugen 5416/hypoxia mouse

Ellen C. Breen, Miriam Scadeng, N. Chin Lai, Fiona Murray, The Late Timothy D. Bigby

Research output: Contribution to journalArticle

2 Citations (Scopus)
5 Downloads (Pure)

Abstract

Pulmonary arterial hypertension (PAH) is characterized by elevated pulmonary artery pressures and right heart failure. PAH mouse models are instrumental in understanding disease pathophysiology. However, few methods are available to evaluate right cardiac function in small animals. In this study, magnetic resonance imaging (MRI) was used to measure in vivo cardiac dimensions in the Sugen 5416/hypoxia mice model of pulmonary hypertension (PH). PH was induced in C57BL/6 mice by three weeks of exposure to 10% oxygen and VEGF receptor inhibition (20 mg/kg SU5416). Control mice were housed in room air and received vehicle (DMSO). Right ventricle pressures (RVP) were recorded with a pressure-conductance transducer. Short axis contiguous 1 mm thick slices were acquired through the heart and great vessels using a Fast Low Angle SHot (FLASH)-cine sequence. Thirteen images were collected throughout each cardiac cycle. RV systolic pressure was elevated in PH and control mice (23.6 ± 5.5 vs. 41.0 ± 11.2 mm Hg, control vs. PH, respectively; p < 0.001, n = 5-11). RV wall thickness was greater in PH mice than control at end-diastole (0.30 ± 0.05, vs. 0.48 + 0.06 mm, control vs. PH, respectively; p < 0.01, n = 6), but measurements were not different at end-systole (control vs. PH, 0.59 ± 0.11 vs. 0.70 + 0.11 mm, respectively). RV ejection fraction was decreased in PH mice (71.9% ± 2.5 vs. 57.6% ± 5.4, control vs. PH, respectively, p < 0.04, n =6). These data demonstrate that MRI is a precise method to longitudinally monitor right ventricle remodeling and cardiac output in mice models of PH.
Original languageEnglish
Pages (from-to)347–353
Number of pages6
JournalExperimental Physiology
Volume102
Issue number3
Early online date14 Feb 2017
DOIs
Publication statusPublished - 1 Mar 2017

Fingerprint

Pulmonary Hypertension
Magnetic Resonance Imaging
Heart Ventricles
Hypoxia
Pressure Transducers
Pressure
Vascular Endothelial Growth Factor Receptor
Ventricular Remodeling
Diastole
Systole
Dimethyl Sulfoxide
Inbred C57BL Mouse
Cardiac Output
Pulmonary Artery
Heart Failure
Air
Oxygen
Blood Pressure

Keywords

  • cardiac output
  • magnetic resonance
  • pulmonary hypertension
  • hypoxia

Cite this

Functional Magnetic Resonance Imaging for In Vivo Quantification of Pulmonary Hypertension in the Sugen 5416/hypoxia mouse. / Breen, Ellen C. ; Scadeng, Miriam; Lai, N. Chin; Murray, Fiona; Bigby, The Late Timothy D.

In: Experimental Physiology, Vol. 102, No. 3, 01.03.2017, p. 347–353 .

Research output: Contribution to journalArticle

Breen, Ellen C. ; Scadeng, Miriam ; Lai, N. Chin ; Murray, Fiona ; Bigby, The Late Timothy D. / Functional Magnetic Resonance Imaging for In Vivo Quantification of Pulmonary Hypertension in the Sugen 5416/hypoxia mouse. In: Experimental Physiology. 2017 ; Vol. 102, No. 3. pp. 347–353 .
@article{87aca92fb70a46119b3b8211c3f27176,
title = "Functional Magnetic Resonance Imaging for In Vivo Quantification of Pulmonary Hypertension in the Sugen 5416/hypoxia mouse",
abstract = "Pulmonary arterial hypertension (PAH) is characterized by elevated pulmonary artery pressures and right heart failure. PAH mouse models are instrumental in understanding disease pathophysiology. However, few methods are available to evaluate right cardiac function in small animals. In this study, magnetic resonance imaging (MRI) was used to measure in vivo cardiac dimensions in the Sugen 5416/hypoxia mice model of pulmonary hypertension (PH). PH was induced in C57BL/6 mice by three weeks of exposure to 10{\%} oxygen and VEGF receptor inhibition (20 mg/kg SU5416). Control mice were housed in room air and received vehicle (DMSO). Right ventricle pressures (RVP) were recorded with a pressure-conductance transducer. Short axis contiguous 1 mm thick slices were acquired through the heart and great vessels using a Fast Low Angle SHot (FLASH)-cine sequence. Thirteen images were collected throughout each cardiac cycle. RV systolic pressure was elevated in PH and control mice (23.6 ± 5.5 vs. 41.0 ± 11.2 mm Hg, control vs. PH, respectively; p < 0.001, n = 5-11). RV wall thickness was greater in PH mice than control at end-diastole (0.30 ± 0.05, vs. 0.48 + 0.06 mm, control vs. PH, respectively; p < 0.01, n = 6), but measurements were not different at end-systole (control vs. PH, 0.59 ± 0.11 vs. 0.70 + 0.11 mm, respectively). RV ejection fraction was decreased in PH mice (71.9{\%} ± 2.5 vs. 57.6{\%} ± 5.4, control vs. PH, respectively, p < 0.04, n =6). These data demonstrate that MRI is a precise method to longitudinally monitor right ventricle remodeling and cardiac output in mice models of PH.",
keywords = "cardiac output, magnetic resonance , pulmonary hypertension, hypoxia",
author = "Breen, {Ellen C.} and Miriam Scadeng and Lai, {N. Chin} and Fiona Murray and Bigby, {The Late Timothy D.}",
note = "Acknowledgements: We would like to take this opportunity to dedicate this study to Professor Timothy Bigby who sadly passed away prior to the submission of the manuscript. Professor Bigby was a kind, generous, supportive and inspirational physician and scientist who made major contributions in the field of pulmonary medicine. Grants The Department of Veterans Affairs, NIH PO1 HL091830, NIH P01 HL66941, NIH HL091061 and the ATS foundation and Pulmonary Hypertension Association supported this study.",
year = "2017",
month = "3",
day = "1",
doi = "10.1113/EP086067",
language = "English",
volume = "102",
pages = "347–353",
journal = "Experimental Physiology",
issn = "0958-0670",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Functional Magnetic Resonance Imaging for In Vivo Quantification of Pulmonary Hypertension in the Sugen 5416/hypoxia mouse

AU - Breen, Ellen C.

AU - Scadeng, Miriam

AU - Lai, N. Chin

AU - Murray, Fiona

AU - Bigby, The Late Timothy D.

N1 - Acknowledgements: We would like to take this opportunity to dedicate this study to Professor Timothy Bigby who sadly passed away prior to the submission of the manuscript. Professor Bigby was a kind, generous, supportive and inspirational physician and scientist who made major contributions in the field of pulmonary medicine. Grants The Department of Veterans Affairs, NIH PO1 HL091830, NIH P01 HL66941, NIH HL091061 and the ATS foundation and Pulmonary Hypertension Association supported this study.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Pulmonary arterial hypertension (PAH) is characterized by elevated pulmonary artery pressures and right heart failure. PAH mouse models are instrumental in understanding disease pathophysiology. However, few methods are available to evaluate right cardiac function in small animals. In this study, magnetic resonance imaging (MRI) was used to measure in vivo cardiac dimensions in the Sugen 5416/hypoxia mice model of pulmonary hypertension (PH). PH was induced in C57BL/6 mice by three weeks of exposure to 10% oxygen and VEGF receptor inhibition (20 mg/kg SU5416). Control mice were housed in room air and received vehicle (DMSO). Right ventricle pressures (RVP) were recorded with a pressure-conductance transducer. Short axis contiguous 1 mm thick slices were acquired through the heart and great vessels using a Fast Low Angle SHot (FLASH)-cine sequence. Thirteen images were collected throughout each cardiac cycle. RV systolic pressure was elevated in PH and control mice (23.6 ± 5.5 vs. 41.0 ± 11.2 mm Hg, control vs. PH, respectively; p < 0.001, n = 5-11). RV wall thickness was greater in PH mice than control at end-diastole (0.30 ± 0.05, vs. 0.48 + 0.06 mm, control vs. PH, respectively; p < 0.01, n = 6), but measurements were not different at end-systole (control vs. PH, 0.59 ± 0.11 vs. 0.70 + 0.11 mm, respectively). RV ejection fraction was decreased in PH mice (71.9% ± 2.5 vs. 57.6% ± 5.4, control vs. PH, respectively, p < 0.04, n =6). These data demonstrate that MRI is a precise method to longitudinally monitor right ventricle remodeling and cardiac output in mice models of PH.

AB - Pulmonary arterial hypertension (PAH) is characterized by elevated pulmonary artery pressures and right heart failure. PAH mouse models are instrumental in understanding disease pathophysiology. However, few methods are available to evaluate right cardiac function in small animals. In this study, magnetic resonance imaging (MRI) was used to measure in vivo cardiac dimensions in the Sugen 5416/hypoxia mice model of pulmonary hypertension (PH). PH was induced in C57BL/6 mice by three weeks of exposure to 10% oxygen and VEGF receptor inhibition (20 mg/kg SU5416). Control mice were housed in room air and received vehicle (DMSO). Right ventricle pressures (RVP) were recorded with a pressure-conductance transducer. Short axis contiguous 1 mm thick slices were acquired through the heart and great vessels using a Fast Low Angle SHot (FLASH)-cine sequence. Thirteen images were collected throughout each cardiac cycle. RV systolic pressure was elevated in PH and control mice (23.6 ± 5.5 vs. 41.0 ± 11.2 mm Hg, control vs. PH, respectively; p < 0.001, n = 5-11). RV wall thickness was greater in PH mice than control at end-diastole (0.30 ± 0.05, vs. 0.48 + 0.06 mm, control vs. PH, respectively; p < 0.01, n = 6), but measurements were not different at end-systole (control vs. PH, 0.59 ± 0.11 vs. 0.70 + 0.11 mm, respectively). RV ejection fraction was decreased in PH mice (71.9% ± 2.5 vs. 57.6% ± 5.4, control vs. PH, respectively, p < 0.04, n =6). These data demonstrate that MRI is a precise method to longitudinally monitor right ventricle remodeling and cardiac output in mice models of PH.

KW - cardiac output

KW - magnetic resonance

KW - pulmonary hypertension

KW - hypoxia

U2 - 10.1113/EP086067

DO - 10.1113/EP086067

M3 - Article

VL - 102

SP - 347

EP - 353

JO - Experimental Physiology

JF - Experimental Physiology

SN - 0958-0670

IS - 3

ER -