Further evidence for the presence of cannabinoid CB1, receptors in mouse vas deferens

Roger Guy Pertwee, G JoeAdigwe, Gabrielle Margaret Hawksworth

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Our results provide further evidence for the hypothesis that the mouse vas deferens contains cannabinoid CB1 receptors. Thus we found that in the presence of forskolin, the cannabinoid receptor agonist, CP 55,940 ((-)-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-4-(3-hydroxypropyl)cyclohexan-1-ol) produced a concentration related inhibition of cyclic AMP production by the vas deferens (EC(50) = 6.0 nM). At 100 nM, SR141716A (N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl- 1H-pyrazole-3-carboxamide hydrochloride) attenuated this effect of CP 55,940, producing a parallel rightward shift in its log concentration-response curve (K-d = 4.3 nM). We also found that cyclic AMP production was inhibited by(-)-11-hydroxy-1',1'-dimethylheptyl-Delta(8)-tetrahydrocannabinol but not by the (+) enantiomer.

Original languageEnglish
Pages (from-to)169-172
Number of pages4
JournalEuropean Journal of Pharmacology
Volume296
Issue number2
DOIs
Publication statusPublished - 25 Jan 1996

Keywords

  • cannabinoid receptor
  • cannabinoid receptor antagonist
  • SR141716A
  • vas deferens, mouse
  • cAMP
  • adenylate cyclase
  • ACCUMULATION
  • Cannabinoid receptor
  • Cannabinoid receptor antagonist;
  • Vas deferens mouse

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