Future perspectives on in-vitro diagnosis of drug allergy by the lymphocyte transformation test

Amol Fatangare, Andreas Glässner, Bernhardt Sachs, Albert Sickmann* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This article aims to envisage future perspectives of the lymphocyte transformation test (LTT). We describe the select innovative techniques, which can be integrated at different stages of the LTT to potentially improve the sensitivity, specificity, or practicability of the LTT. We first focus upon the cell sorting techniques comprising immunomagnetic cell separation and flow cytometry, which can be implemented prior and after the LTT culturing step to concentrate and quantify specific immune cell types. Further, we elaborate upon three important omics techniques such as transcriptomics, proteomics, and metabolomics, which can be integrated downstream of the LTT to analyze molecular changes in specific immune cells following drug induced activation and proliferation. We also develop visions, how state of the art techniques used in other scientific fields, can be transferred and applied in the context of in-vitro detection of drug allergy. [Abstract copyright: Copyright © 2021. Published by Elsevier B.V.]
Original languageEnglish
Article number113072
Number of pages7
JournalJournal of immunological methods
Volume495
Early online date14 May 2021
DOIs
Publication statusE-pub ahead of print - 14 May 2021

Keywords

  • Cell sorting by immunomagnetic cell separation and flow cytometry
  • Drug allergy
  • Future perspectives in vitro diagnostics
  • Lymphocyte transformation test (LTT)
  • Omics by transcriptomics proteomics and metabolomics
  • T cell activation and proliferation

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