Pulmonary arterial hypertension (PAH) is characterized by increased pulmonary vascular resistance, in part due to increased proliferation of pulmonary artery smooth muscle cells (PASMC). Since 3’5’-cyclic adenosine monophosphate (cAMP) decreases proliferation of PASMC, G protein-coupled receptors (GPCRs) that couple to Gαs or Gαi are attractive targets for the treatment of PAH. We used a real-time PCR GPCR array to identify and quantify the GPCRs expressed by PASMC isolated from normal subjects and from patients with PAH. We found that human PASMC express >135 GPCRs, at least 60 of which regulate cAMP formation. GPCR expression correlates with function: for Gαs-coupled GPCRs with formation of cAMP and inhibition of cell proliferation, thus documenting that the GPCR array identifies physiologically relevant GPCRs. PAH-PASMC (both from idiopathic and secondary PAH patients) have an increase (>2-fold) in the expression of 41 GPCRs compared to control-PASMC, including multiple GPCRs that link to Gαs or Gαi. In addition, PAH-PASMC have a higher expression of GPCRs that preferentially couple to Gαq/11 or to Gα12/13. Taken together these data provide evidence that a GPCRomic approach can identify GPCRs that may contribute to the physiology of PASMC and that may be new druggable targets for PAH, a disease for which no currently optimal therapies exist.
|Number of pages||1|
|Journal||The FASEB Journal|
|Issue number||Suppl 1|
|Publication status||Published - Apr 2014|
McDonald, D., Aroonsakool, N., Kwon, O., Insel, P., & Murray, F. (2014). G protein-coupled receptor expression and function in pulmonary artery smooth muscle cells: new targets in pulmonary arterial hypertension. The FASEB Journal, 28(Suppl 1), [1090.3]. http://www.fasebj.org/content/28/1_Supplement/1090.3.short