GABAA cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1

F. K. Bedford, J. T. Kittler, E. Muller, P. Thomas, J. M. Uren, D. Merlo, William Wisden, A. Triller, T. G. Smart, S. J. Moss

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    Abstract

    Controlling the number of functional gamma -aminobutyric acid A (GABA(A)) receptors in neuronal membranes is a crucial factor for the efficacy of inhibitory neurotransmission. Here we describe the direct interaction of GABA(A) receptors with the ubiquitin-like protein Plic-1. Furthermore, Plic-1 is enriched at inhibitory synapses and is associated with subsynaptic membranes. Functionally, Plic-1 facilitates GABA(A) receptor cell surface expression without affecting the rate of receptor internalization. Plic-1 also enhances the stability of intracellular GABA(A) receptor subunits, increasing the number of receptors available for insertion into the plasma membrane. Our study identifies a previously unknown role for Plic-1, a modulation of GABA(A) receptor cell surface number, which suggests that Plic-1 facilitates accumulation of these receptors in dendritic membranes.

    Original languageEnglish
    Pages (from-to)908-916
    Number of pages8
    JournalNature Neuroscience
    Volume4
    Issue number9
    DOIs
    Publication statusPublished - Sep 2001

    Keywords

    • RAT SPINAL-CORD
    • HIPPOCAMPAL-NEURONS
    • GAMMA-2 SUBUNIT
    • A RECEPTORS
    • AMPA RECEPTORS
    • BETA SUBUNITS
    • SYNAPSES
    • IDENTIFICATION
    • ENDOCYTOSIS
    • EXPRESSION

    Cite this

    Bedford, F. K., Kittler, J. T., Muller, E., Thomas, P., Uren, J. M., Merlo, D., Wisden, W., Triller, A., Smart, T. G., & Moss, S. J. (2001). GABAA cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1. Nature Neuroscience, 4(9), 908-916. https://doi.org/10.1038/nn0901-908