GABAA cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1

F. K. Bedford, J. T. Kittler, E. Muller, P. Thomas, J. M. Uren, D. Merlo, William Wisden, A. Triller, T. G. Smart, S. J. Moss

    Research output: Contribution to journalArticle

    186 Citations (Scopus)

    Abstract

    Controlling the number of functional gamma -aminobutyric acid A (GABA(A)) receptors in neuronal membranes is a crucial factor for the efficacy of inhibitory neurotransmission. Here we describe the direct interaction of GABA(A) receptors with the ubiquitin-like protein Plic-1. Furthermore, Plic-1 is enriched at inhibitory synapses and is associated with subsynaptic membranes. Functionally, Plic-1 facilitates GABA(A) receptor cell surface expression without affecting the rate of receptor internalization. Plic-1 also enhances the stability of intracellular GABA(A) receptor subunits, increasing the number of receptors available for insertion into the plasma membrane. Our study identifies a previously unknown role for Plic-1, a modulation of GABA(A) receptor cell surface number, which suggests that Plic-1 facilitates accumulation of these receptors in dendritic membranes.

    Original languageEnglish
    Pages (from-to)908-916
    Number of pages8
    JournalNature Neuroscience
    Volume4
    Issue number9
    DOIs
    Publication statusPublished - Sep 2001

    Keywords

    • RAT SPINAL-CORD
    • HIPPOCAMPAL-NEURONS
    • GAMMA-2 SUBUNIT
    • A RECEPTORS
    • AMPA RECEPTORS
    • BETA SUBUNITS
    • SYNAPSES
    • IDENTIFICATION
    • ENDOCYTOSIS
    • EXPRESSION

    Cite this

    Bedford, F. K., Kittler, J. T., Muller, E., Thomas, P., Uren, J. M., Merlo, D., ... Moss, S. J. (2001). GABAA cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1. Nature Neuroscience, 4(9), 908-916. https://doi.org/10.1038/nn0901-908

    GABAA cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1. / Bedford, F. K.; Kittler, J. T.; Muller, E.; Thomas, P.; Uren, J. M.; Merlo, D.; Wisden, William; Triller, A.; Smart, T. G.; Moss, S. J.

    In: Nature Neuroscience, Vol. 4, No. 9, 09.2001, p. 908-916.

    Research output: Contribution to journalArticle

    Bedford, FK, Kittler, JT, Muller, E, Thomas, P, Uren, JM, Merlo, D, Wisden, W, Triller, A, Smart, TG & Moss, SJ 2001, 'GABAA cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1' Nature Neuroscience, vol. 4, no. 9, pp. 908-916. https://doi.org/10.1038/nn0901-908
    Bedford, F. K. ; Kittler, J. T. ; Muller, E. ; Thomas, P. ; Uren, J. M. ; Merlo, D. ; Wisden, William ; Triller, A. ; Smart, T. G. ; Moss, S. J. / GABAA cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1. In: Nature Neuroscience. 2001 ; Vol. 4, No. 9. pp. 908-916.
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