Gain-of-Function and Loss-of-Function Strategies in Xenopus

Danielle Lavery, Stefan Hoppler

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Xenopus embryos are particularly suited for functional experiments to investigate vertebrate embryonic development. Due to the large size of embryos and their development outside of the mother organism, they are very accessible, easy to manipulate, and allow for immediate observation of developmental phenotypes. Powerful methods have been established for both gain- and loss-of-function strategies, which build on these inherent advantages. This chapter describes injection methods used to overexpress gene products and inhibit gene expression as well as pharmacological approaches to manipulate Wnt signaling in Xenopus embryos.
Original languageEnglish
Title of host publicationWnt Signaling
Subtitle of host publicationVolume 2, Pathway Models
EditorsElizabeth Vincan
Place of PublicationTotowa, NJ, USA
PublisherHumana Press
Pages401-415
Number of pages15
Volume469 (2)
ISBN (Print)1603274685 , 978-1603274685
DOIs
Publication statusPublished - 23 Oct 2008

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Number2
Volume469
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • xenopus
  • microinjection
  • morpholino
  • small molecule inhibitors
  • BIO

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    Lavery, D., & Hoppler, S. (2008). Gain-of-Function and Loss-of-Function Strategies in Xenopus. In E. Vincan (Ed.), Wnt Signaling: Volume 2, Pathway Models (Vol. 469 (2), pp. 401-415). (Methods in Molecular Biology; Vol. 469, No. 2). Humana Press. https://doi.org/10.1007/978-1-60327-469-25