Gene and protein expression profiles in the foetal liver of the pregnant rat fed a low protein diet

Christopher J McNeil, Susan M Hay, Garry J Rucklidge, Martin D Reid, Gary J Duncan, William David Rees

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Foetal growth is particularly sensitive to the protein content of the mother's diet. Microarray data from the foetal liver of pregnant rats fed normal (HP) or reduced protein diets (LP) were compared by gene set enrichment analysis. Soluble proteins from a second portion of the liver were analysed by two-dimensional gel electrophoresis. Genes associated with progesterone, insulin-like growth factor-1 and vascular endothelial growth factor were upregulated in HP compared to LP, in addition to genes associated with cell differentiation and signalling from the extracellular matrix. In contrast, cytokine signalling was downregulated. Proteomics showed that proteins associated with amino acid metabolism, mitochondrial function and cell motility were differentially abundant in the HP compared to the LP groups. These growth factor and extracellular matrix signalling pathways linked to cell motility may be important mediators of the changes in liver structure that occur in utero and persist into adult life.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalGenes & Nutrition
Volume4
Issue number3
Early online date30 May 2009
DOIs
Publication statusPublished - Sep 2009

Keywords

  • programming
  • hepatic development
  • metabolism
  • foetal origins of disease
  • lipid-metabolism
  • growth
  • restriction
  • hepatocytes
  • endocrine
  • fetus
  • life

Cite this

Gene and protein expression profiles in the foetal liver of the pregnant rat fed a low protein diet. / McNeil, Christopher J; Hay, Susan M; Rucklidge, Garry J; Reid, Martin D; Duncan, Gary J; Rees, William David.

In: Genes & Nutrition, Vol. 4, No. 3, 09.2009, p. 189-194.

Research output: Contribution to journalArticle

McNeil, Christopher J ; Hay, Susan M ; Rucklidge, Garry J ; Reid, Martin D ; Duncan, Gary J ; Rees, William David. / Gene and protein expression profiles in the foetal liver of the pregnant rat fed a low protein diet. In: Genes & Nutrition. 2009 ; Vol. 4, No. 3. pp. 189-194.
@article{574e9fed2f8741f4a3e7bac4a1758805,
title = "Gene and protein expression profiles in the foetal liver of the pregnant rat fed a low protein diet",
abstract = "Foetal growth is particularly sensitive to the protein content of the mother's diet. Microarray data from the foetal liver of pregnant rats fed normal (HP) or reduced protein diets (LP) were compared by gene set enrichment analysis. Soluble proteins from a second portion of the liver were analysed by two-dimensional gel electrophoresis. Genes associated with progesterone, insulin-like growth factor-1 and vascular endothelial growth factor were upregulated in HP compared to LP, in addition to genes associated with cell differentiation and signalling from the extracellular matrix. In contrast, cytokine signalling was downregulated. Proteomics showed that proteins associated with amino acid metabolism, mitochondrial function and cell motility were differentially abundant in the HP compared to the LP groups. These growth factor and extracellular matrix signalling pathways linked to cell motility may be important mediators of the changes in liver structure that occur in utero and persist into adult life.",
keywords = "programming, hepatic development, metabolism, foetal origins of disease, lipid-metabolism, growth, restriction, hepatocytes, endocrine, fetus, life",
author = "McNeil, {Christopher J} and Hay, {Susan M} and Rucklidge, {Garry J} and Reid, {Martin D} and Duncan, {Gary J} and Rees, {William David}",
year = "2009",
month = "9",
doi = "10.1007/s12263-009-0125-6",
language = "English",
volume = "4",
pages = "189--194",
journal = "Genes & Nutrition",
issn = "1555-8932",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Gene and protein expression profiles in the foetal liver of the pregnant rat fed a low protein diet

AU - McNeil, Christopher J

AU - Hay, Susan M

AU - Rucklidge, Garry J

AU - Reid, Martin D

AU - Duncan, Gary J

AU - Rees, William David

PY - 2009/9

Y1 - 2009/9

N2 - Foetal growth is particularly sensitive to the protein content of the mother's diet. Microarray data from the foetal liver of pregnant rats fed normal (HP) or reduced protein diets (LP) were compared by gene set enrichment analysis. Soluble proteins from a second portion of the liver were analysed by two-dimensional gel electrophoresis. Genes associated with progesterone, insulin-like growth factor-1 and vascular endothelial growth factor were upregulated in HP compared to LP, in addition to genes associated with cell differentiation and signalling from the extracellular matrix. In contrast, cytokine signalling was downregulated. Proteomics showed that proteins associated with amino acid metabolism, mitochondrial function and cell motility were differentially abundant in the HP compared to the LP groups. These growth factor and extracellular matrix signalling pathways linked to cell motility may be important mediators of the changes in liver structure that occur in utero and persist into adult life.

AB - Foetal growth is particularly sensitive to the protein content of the mother's diet. Microarray data from the foetal liver of pregnant rats fed normal (HP) or reduced protein diets (LP) were compared by gene set enrichment analysis. Soluble proteins from a second portion of the liver were analysed by two-dimensional gel electrophoresis. Genes associated with progesterone, insulin-like growth factor-1 and vascular endothelial growth factor were upregulated in HP compared to LP, in addition to genes associated with cell differentiation and signalling from the extracellular matrix. In contrast, cytokine signalling was downregulated. Proteomics showed that proteins associated with amino acid metabolism, mitochondrial function and cell motility were differentially abundant in the HP compared to the LP groups. These growth factor and extracellular matrix signalling pathways linked to cell motility may be important mediators of the changes in liver structure that occur in utero and persist into adult life.

KW - programming

KW - hepatic development

KW - metabolism

KW - foetal origins of disease

KW - lipid-metabolism

KW - growth

KW - restriction

KW - hepatocytes

KW - endocrine

KW - fetus

KW - life

U2 - 10.1007/s12263-009-0125-6

DO - 10.1007/s12263-009-0125-6

M3 - Article

VL - 4

SP - 189

EP - 194

JO - Genes & Nutrition

JF - Genes & Nutrition

SN - 1555-8932

IS - 3

ER -