Gene expression analysis of human fetal ovarian primordial follicle formation

Paul A. Fowler, Samantha Flannigan, Anna Mathers, Kim Gillanders, Richard G. Lea, Maureen J. Wood, Abha Maheshwari, Siladitya Bhattacharya, Elaina S. R. Collie-Duguid, Paul J. Baker, Ana Monteiro, Peter J. O'Shaughnessy

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Context: Primordial follicle formation dictates the maximal potential female reproductive capacity and establishes the ovarian reserve. Currently, little is known about this process in the human.

Objective: The aim of the study was to identify genes associated with the onset of human fetal primordial follicle formation in morphologically normal human fetuses.

Design: We conducted an observational study of the female fetal gonad, comparing gene expression before and during primordial follicle formation.

Setting: The study was conducted at the Universities of Aberdeen, Glasgow, and Nottingham.

Patients/Participants: Ovaries were collected from 51 morphologically normal human female fetuses of women undergoing elective termination of normal second trimester pregnancies.

Main Outcome Measures: We performed fetal ovarian transcript expression by Affymetrix array and quantitative RT-PCR and gene product expression and localization by Western blot and immunohistochemistry.

Results: Five transcripts were down-regulated and 61 were up-regulated in ovaries from older fetuses (18–20 wk) in which primordial follicle formation had started compared with younger (15–16 wk) fetuses in which no primordial follicles were observed. The altered genes contribute to major functions, including gene expression, tissue morphology, and apoptosis, that are essential for ovarian development. NALP5, the most highly regulated transcript, is an oocyte-specific maternal effect gene that is regulated downstream of FIGLA.

Conclusions: NALP5 probably plays a key role in the onset of human primordial follicle formation and thus the establishment of ovarian reserve in women.
Original languageEnglish
Pages (from-to)1427-1435
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume94
Issue number4
Early online date3 Mar 2009
DOIs
Publication statusPublished - 1 Apr 2009

Keywords

  • early embryonic-development
  • transcription factor
  • zona-pellucida
  • mouse ovary
  • regulated genes
  • somatic-cells
  • gem-cells
  • in-vitro
  • oocytes
  • folliculogenesis

Cite this

Fowler, P. A., Flannigan, S., Mathers, A., Gillanders, K., Lea, R. G., Wood, M. J., ... O'Shaughnessy, P. J. (2009). Gene expression analysis of human fetal ovarian primordial follicle formation. Journal of Clinical Endocrinology and Metabolism, 94(4), 1427-1435. https://doi.org/10.1210/jc.2008-2619

Gene expression analysis of human fetal ovarian primordial follicle formation. / Fowler, Paul A.; Flannigan, Samantha; Mathers, Anna; Gillanders, Kim; Lea, Richard G.; Wood, Maureen J.; Maheshwari, Abha; Bhattacharya, Siladitya; Collie-Duguid, Elaina S. R.; Baker, Paul J.; Monteiro, Ana; O'Shaughnessy, Peter J.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 94, No. 4, 01.04.2009, p. 1427-1435.

Research output: Contribution to journalArticle

Fowler, Paul A. ; Flannigan, Samantha ; Mathers, Anna ; Gillanders, Kim ; Lea, Richard G. ; Wood, Maureen J. ; Maheshwari, Abha ; Bhattacharya, Siladitya ; Collie-Duguid, Elaina S. R. ; Baker, Paul J. ; Monteiro, Ana ; O'Shaughnessy, Peter J. / Gene expression analysis of human fetal ovarian primordial follicle formation. In: Journal of Clinical Endocrinology and Metabolism. 2009 ; Vol. 94, No. 4. pp. 1427-1435.
@article{7aba63e122fc456195f8d814a98fa396,
title = "Gene expression analysis of human fetal ovarian primordial follicle formation",
abstract = "Context: Primordial follicle formation dictates the maximal potential female reproductive capacity and establishes the ovarian reserve. Currently, little is known about this process in the human. Objective: The aim of the study was to identify genes associated with the onset of human fetal primordial follicle formation in morphologically normal human fetuses. Design: We conducted an observational study of the female fetal gonad, comparing gene expression before and during primordial follicle formation. Setting: The study was conducted at the Universities of Aberdeen, Glasgow, and Nottingham. Patients/Participants: Ovaries were collected from 51 morphologically normal human female fetuses of women undergoing elective termination of normal second trimester pregnancies. Main Outcome Measures: We performed fetal ovarian transcript expression by Affymetrix array and quantitative RT-PCR and gene product expression and localization by Western blot and immunohistochemistry. Results: Five transcripts were down-regulated and 61 were up-regulated in ovaries from older fetuses (18–20 wk) in which primordial follicle formation had started compared with younger (15–16 wk) fetuses in which no primordial follicles were observed. The altered genes contribute to major functions, including gene expression, tissue morphology, and apoptosis, that are essential for ovarian development. NALP5, the most highly regulated transcript, is an oocyte-specific maternal effect gene that is regulated downstream of FIGLA. Conclusions: NALP5 probably plays a key role in the onset of human primordial follicle formation and thus the establishment of ovarian reserve in women.",
keywords = "early embryonic-development, transcription factor, zona-pellucida, mouse ovary, regulated genes, somatic-cells, gem-cells, in-vitro, oocytes, folliculogenesis",
author = "Fowler, {Paul A.} and Samantha Flannigan and Anna Mathers and Kim Gillanders and Lea, {Richard G.} and Wood, {Maureen J.} and Abha Maheshwari and Siladitya Bhattacharya and Collie-Duguid, {Elaina S. R.} and Baker, {Paul J.} and Ana Monteiro and O'Shaughnessy, {Peter J.}",
year = "2009",
month = "4",
day = "1",
doi = "10.1210/jc.2008-2619",
language = "English",
volume = "94",
pages = "1427--1435",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - Gene expression analysis of human fetal ovarian primordial follicle formation

AU - Fowler, Paul A.

AU - Flannigan, Samantha

AU - Mathers, Anna

AU - Gillanders, Kim

AU - Lea, Richard G.

AU - Wood, Maureen J.

AU - Maheshwari, Abha

AU - Bhattacharya, Siladitya

AU - Collie-Duguid, Elaina S. R.

AU - Baker, Paul J.

AU - Monteiro, Ana

AU - O'Shaughnessy, Peter J.

PY - 2009/4/1

Y1 - 2009/4/1

N2 - Context: Primordial follicle formation dictates the maximal potential female reproductive capacity and establishes the ovarian reserve. Currently, little is known about this process in the human. Objective: The aim of the study was to identify genes associated with the onset of human fetal primordial follicle formation in morphologically normal human fetuses. Design: We conducted an observational study of the female fetal gonad, comparing gene expression before and during primordial follicle formation. Setting: The study was conducted at the Universities of Aberdeen, Glasgow, and Nottingham. Patients/Participants: Ovaries were collected from 51 morphologically normal human female fetuses of women undergoing elective termination of normal second trimester pregnancies. Main Outcome Measures: We performed fetal ovarian transcript expression by Affymetrix array and quantitative RT-PCR and gene product expression and localization by Western blot and immunohistochemistry. Results: Five transcripts were down-regulated and 61 were up-regulated in ovaries from older fetuses (18–20 wk) in which primordial follicle formation had started compared with younger (15–16 wk) fetuses in which no primordial follicles were observed. The altered genes contribute to major functions, including gene expression, tissue morphology, and apoptosis, that are essential for ovarian development. NALP5, the most highly regulated transcript, is an oocyte-specific maternal effect gene that is regulated downstream of FIGLA. Conclusions: NALP5 probably plays a key role in the onset of human primordial follicle formation and thus the establishment of ovarian reserve in women.

AB - Context: Primordial follicle formation dictates the maximal potential female reproductive capacity and establishes the ovarian reserve. Currently, little is known about this process in the human. Objective: The aim of the study was to identify genes associated with the onset of human fetal primordial follicle formation in morphologically normal human fetuses. Design: We conducted an observational study of the female fetal gonad, comparing gene expression before and during primordial follicle formation. Setting: The study was conducted at the Universities of Aberdeen, Glasgow, and Nottingham. Patients/Participants: Ovaries were collected from 51 morphologically normal human female fetuses of women undergoing elective termination of normal second trimester pregnancies. Main Outcome Measures: We performed fetal ovarian transcript expression by Affymetrix array and quantitative RT-PCR and gene product expression and localization by Western blot and immunohistochemistry. Results: Five transcripts were down-regulated and 61 were up-regulated in ovaries from older fetuses (18–20 wk) in which primordial follicle formation had started compared with younger (15–16 wk) fetuses in which no primordial follicles were observed. The altered genes contribute to major functions, including gene expression, tissue morphology, and apoptosis, that are essential for ovarian development. NALP5, the most highly regulated transcript, is an oocyte-specific maternal effect gene that is regulated downstream of FIGLA. Conclusions: NALP5 probably plays a key role in the onset of human primordial follicle formation and thus the establishment of ovarian reserve in women.

KW - early embryonic-development

KW - transcription factor

KW - zona-pellucida

KW - mouse ovary

KW - regulated genes

KW - somatic-cells

KW - gem-cells

KW - in-vitro

KW - oocytes

KW - folliculogenesis

U2 - 10.1210/jc.2008-2619

DO - 10.1210/jc.2008-2619

M3 - Article

VL - 94

SP - 1427

EP - 1435

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 4

ER -