Gene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokines

A. Attaya, T. Wang, J. Zou, T. Herath, A. Adams, C. J. Secombes*, S. Yoon

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)
3 Downloads (Pure)

Abstract

Increased knowledge of the immune response of the intestine, a physiologically critical organ involved in absorption, secretion and homeostasis in a non-sterile environment, is needed to better understand the mechanisms involved in the induction of long-lasting immunity and, subsequently, the development of efficacious gastrointestinal immunization approaches. To this end, analysis of isolated gut cells will give an insight into the cell types present and their immune capability. Hence, in this study we first optimised a method for salmonid gut leucocyte isolation and characterised the cells on the basis of their expression of a range of selected cell markers associated with T & B cells and dendritic cells. The GALT leucocytes were then stimulated with a variety of PAMPs, recombinant cytokines and PHA, as a means to help characterise the diversity of the immune repertoire present in such cells. The stimulants tested were designed to examine the nature of the antibacterial, antiviral and T cell type responses in the cells (at the transcript level) using a panel of genes relevant to innate and adaptive immunity. The results showed distinct responses to the stimulants, with a clear delineation seen between the stimulant used (eg viral or bacterial PAMP) and the pathway elicited. The changes in the expression patterns of the immune genes in these cells indicates that the salmonid intestine contains a good repertoire of competent immune cells able to respond to different pathogen types. Such information may aid the development of efficient priming by oral vaccination in salmonids.

Original languageEnglish
Pages (from-to)426-436
Number of pages11
JournalFish & Shellfish Immunology
Volume80
Early online date12 Jun 2018
DOIs
Publication statusPublished - 1 Sep 2018

Keywords

  • GALT leucocytes
  • Isolation
  • Salmonids
  • PAMPs
  • Recombinant cytokines
  • Gene expression

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

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