Gene expression profiles, phenolics and lignin of Sitka spruce bark and sapwood before and after wounding and inoculation with Heterobasidion annosum

Giuliana Deflorio, Graham Horgan, Stephen Woodward, Carl Gunnar Fossdal

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Gene expression levels (PAL, CCR1, HCT1, and CAD for the phenylpropanoid pathway, PX3 peroxidase,and CHI4 class IV chitinase), lignin, and soluble and cell wall bound phenolic compounds in bark andsapwood of
Picea sitchensis
clones inoculated withHeterobasidion annosum s.s.were compared beforeand 3 days after wounding and arti¿cial inoculation, at site of inoculation and 1 cm above the inoculationsite.In bark all genes were up-regulated at the site of inoculation but, except for CAD, not in the distal zone.In sapwood all genes were down-regulated, except for PX3 and CHI4; PAL, CCR1, HCT1 and CAD werepresent at lower levels around the inoculation site than in the distal zone. Compared to wounding only,inoculation withH. annosumtriggered different CAD, PX3, and CHI4 levels in bark but not in sapwood.Different concentrations of cell wall bound phenolic compounds (unknown2, unknown3, coniferin,astringin, taxifolin, piceid, and isorhapontin) were found in bark after wounding and inoculationcompared to constitutive material (i.e. untreated samples), whereas in sapwood concentrations did notdiffer following treatment. These results indicate that bark of Sitka spruce has a stronger and earlierresponse to wounding and pathogen inoculation than sapwood.
Original languageEnglish
Pages (from-to)180-187
Number of pages8
JournalPhysiological & Molecular Plant Pathology
Volume75
Issue number4
DOIs
Publication statusPublished - Apr 2011

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Heterobasidion annosum
Picea sitchensis
Lignin
sapwood
Transcriptome
Cell Wall
lignin
bark
Picea
Chitinases
gene expression
Peroxidase
Genes
Clone Cells
phenolic compounds
Gene Expression
cell walls
taxifolin
phenylpropanoids
chitinase

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Gene expression profiles, phenolics and lignin of Sitka spruce bark and sapwood before and after wounding and inoculation with Heterobasidion annosum. / Deflorio, Giuliana; Horgan, Graham; Woodward, Stephen; Fossdal, Carl Gunnar.

In: Physiological & Molecular Plant Pathology, Vol. 75, No. 4, 04.2011, p. 180-187.

Research output: Contribution to journalArticle

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AB - Gene expression levels (PAL, CCR1, HCT1, and CAD for the phenylpropanoid pathway, PX3 peroxidase,and CHI4 class IV chitinase), lignin, and soluble and cell wall bound phenolic compounds in bark andsapwood of Picea sitchensis clones inoculated withHeterobasidion annosum s.s.were compared beforeand 3 days after wounding and arti¿cial inoculation, at site of inoculation and 1 cm above the inoculationsite.In bark all genes were up-regulated at the site of inoculation but, except for CAD, not in the distal zone.In sapwood all genes were down-regulated, except for PX3 and CHI4; PAL, CCR1, HCT1 and CAD werepresent at lower levels around the inoculation site than in the distal zone. Compared to wounding only,inoculation withH. annosumtriggered different CAD, PX3, and CHI4 levels in bark but not in sapwood.Different concentrations of cell wall bound phenolic compounds (unknown2, unknown3, coniferin,astringin, taxifolin, piceid, and isorhapontin) were found in bark after wounding and inoculationcompared to constitutive material (i.e. untreated samples), whereas in sapwood concentrations did notdiffer following treatment. These results indicate that bark of Sitka spruce has a stronger and earlierresponse to wounding and pathogen inoculation than sapwood.

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