Generating genomic platforms to study Candida albicans pathogenesis

Mélanie Legrand, Sophie Bachellier-Bassi, Keunsook K. Lee, Yogesh Chaudhari, Hélène Tournu, Laurence Arbogast, Hélène Boyer, Murielle Chauvel, Vitor Cabral, Corinne Maufrais, Audrey Nesseir, Irena Maslanka, Emmanuelle Permal, Tristan Rossignol, Louise A. Walker, Ute Zeidler, Sadri Znaidi, Floris Schoeters, Charlotte Majgier, Renaud A. JulienLaurence Ma, Magali Tichit, Christiane Bouchier, Patrick Van Dijck, Carol A. Munro (Corresponding Author), Christophe d'Enfert (Corresponding Author)

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)
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The advent of the genomic era has made elucidating gene function on a large scale a pressing challenge. ORFeome collections, whereby almost all ORFs of a given species are cloned and can be subsequently leveraged in multiple functional genomic approaches, represent valuable resources towards this endeavor. Here we provide novel, genome-scale tools for the study of
Candida albicans, a commensal yeast that is also responsible for frequent superficial and disseminated infections in humans. We have generated an ORFeome collection composed of 5,099 ORFs cloned in a Gateway™ donor vector, representing 83% of the currently annotated coding sequences of C. albicans. Sequencing data of the cloned ORFs are available in the CandidaOrfDB database at We also engineered 49 expression vectors with a choice of promoters, tags, and selection markers and demonstrated their applicability to the study of target ORFs transferred from the
C. albicans ORFeome. In addition, the use of the ORFeome in the detection of protein-protein interaction was demonstrated. Mating-compatible strains as well as Gateway™-compatible two-hybrid vectors were engineered, validated and used in a proof of concept experiment. These unique and valuable resources should greatly facilitate future functional studies in C. albicans and the elucidation of mechanisms that underlie its pathogenicity.
Original languageEnglish
Pages (from-to)6935-6949
Number of pages14
JournalNucleic Acids Research
Issue number14
Early online date6 Jul 2018
Publication statusPublished - 21 Aug 2018


  • ORFeome
  • Candida albicans
  • Two-hybrid system
  • Gateway recombination cloning system
  • Database


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