Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 132-145 |
| Number of pages | 14 |
| Journal | Physica. A, Statistical Mechanics and its Applications |
| Volume | 470 |
| Early online date | 5 Dec 2016 |
| DOIs | |
| Publication status | Published - 15 Mar 2017 |
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Keywords
- Microfluidics
- Gradient
- Diffusion
- Yeast
- Saccharomyces cerevisiae
Cite this
Generation and precise control of dynamic biochemical gradients for cellular assays. / Saka, Yasushi; MacPherson, Murray; Giuraniuc, Claudiu V.
In: Physica. A, Statistical Mechanics and its Applications, Vol. 470, 15.03.2017, p. 132-145.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Generation and precise control of dynamic biochemical gradients for cellular assays
AU - Saka, Yasushi
AU - MacPherson, Murray
AU - Giuraniuc, Claudiu V.
N1 - Acknowledgements We thank Marco Thiel and Alessandro De Moura for helpful discussions and advice, Stefan Hoppler and Mamen Romano for critical reading of the manuscript, James Hislop for his help with the plasma cleaner, Alex Brand for the microscopy imaging system and Alistair Robertson for fabricating the hydrostatic flow controllers. We also thank Diane Massie and Yvonne Turnbull for technical assistance. This work was supported by Scottish Universities Life Sciences Alliance (SULSA)and the University of Aberdeen .
PY - 2017/3/15
Y1 - 2017/3/15
N2 - Spatial gradients of diffusible signalling molecules play crucial roles in controlling diverse cellular behaviour such as cell differentiation, tissue patterning and chemotaxis. In this paper, we report the design and testing of a microfluidic device for diffusionbased gradient generation for cellular assays. A unique channel design of the device eliminates cross-flow between the source and sink channels, thereby stabilising gradients by passive diffusion. The platform also enables quick and flexible control of chemical concentration that makes highly dynamic gradients in diffusion chambers. A model with the first approximation of diffusion and surface adsorption of molecules recapitulates the experimentally observed gradients. Budding yeast cells cultured in a gradient of a chemical inducer expressed a reporter fluorescence protein in a concentration dependent manner. This microfluidic platform serves as a versatile prototype applicable to a broad range of biomedical investigations.
AB - Spatial gradients of diffusible signalling molecules play crucial roles in controlling diverse cellular behaviour such as cell differentiation, tissue patterning and chemotaxis. In this paper, we report the design and testing of a microfluidic device for diffusionbased gradient generation for cellular assays. A unique channel design of the device eliminates cross-flow between the source and sink channels, thereby stabilising gradients by passive diffusion. The platform also enables quick and flexible control of chemical concentration that makes highly dynamic gradients in diffusion chambers. A model with the first approximation of diffusion and surface adsorption of molecules recapitulates the experimentally observed gradients. Budding yeast cells cultured in a gradient of a chemical inducer expressed a reporter fluorescence protein in a concentration dependent manner. This microfluidic platform serves as a versatile prototype applicable to a broad range of biomedical investigations.
KW - Microfluidics
KW - Gradient
KW - Diffusion
KW - Yeast
KW - Saccharomyces cerevisiae
U2 - 10.1016/j.physa.2016.11.134
DO - 10.1016/j.physa.2016.11.134
M3 - Article
VL - 470
SP - 132
EP - 145
JO - Physica. A, Statistical Mechanics and its Applications
JF - Physica. A, Statistical Mechanics and its Applications
SN - 0378-4371
ER -