Generation and precise control of dynamic biochemical gradients for cellular assays

Yasushi Saka; Murray MacPherson; Claudiu V. Giuraniuc

doi:10.1016/j.physa.2016.11.134

Generation and precise control of dynamic biochemical gradients for cellular assays

Yasushi Saka, Murray MacPherson, Claudiu V. Giuraniuc

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Abstract

Spatial gradients of diffusible signalling molecules play crucial roles in controlling diverse cellular behaviour such as cell differentiation, tissue patterning and chemotaxis. In this paper, we report the design and testing of a microfluidic device for diffusionbased gradient generation for cellular assays. A unique channel design of the device eliminates cross-flow between the source and sink channels, thereby stabilising gradients by passive diffusion. The platform also enables quick and flexible control of chemical concentration that makes highly dynamic gradients in diffusion chambers. A model with the first approximation of diffusion and surface adsorption of molecules recapitulates the experimentally observed gradients. Budding yeast cells cultured in a gradient of a chemical inducer expressed a reporter fluorescence protein in a concentration dependent manner. This microfluidic platform serves as a versatile prototype applicable to a broad range of biomedical investigations.

Original language	English
Pages (from-to)	132-145
Number of pages	14
Journal	Physica. A, Statistical Mechanics and its Applications
Volume	470
Early online date	5 Dec 2016
DOIs	https://doi.org/10.1016/j.physa.2016.11.134
Publication status	Published - 15 Mar 2017

Keywords

Microfluidics
Gradient
Diffusion
Yeast
Saccharomyces cerevisiae

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Accepted Manuscript
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Accepted author manuscript, 6.62 MBLicence: CC BY-NC-ND

Vasile Giuraniuc
- School of Medicine, Medical Sciences & Nutrition, Medical Sciences - Research Fellow
- Institute of Medical Sciences
Person: Academic Related - Research

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title = "Generation and precise control of dynamic biochemical gradients for cellular assays",

abstract = "Spatial gradients of diffusible signalling molecules play crucial roles in controlling diverse cellular behaviour such as cell differentiation, tissue patterning and chemotaxis. In this paper, we report the design and testing of a microfluidic device for diffusionbased gradient generation for cellular assays. A unique channel design of the device eliminates cross-flow between the source and sink channels, thereby stabilising gradients by passive diffusion. The platform also enables quick and flexible control of chemical concentration that makes highly dynamic gradients in diffusion chambers. A model with the first approximation of diffusion and surface adsorption of molecules recapitulates the experimentally observed gradients. Budding yeast cells cultured in a gradient of a chemical inducer expressed a reporter fluorescence protein in a concentration dependent manner. This microfluidic platform serves as a versatile prototype applicable to a broad range of biomedical investigations. ",

keywords = "Microfluidics , Gradient , Diffusion , Yeast , Saccharomyces cerevisiae ",

author = "Yasushi Saka and Murray MacPherson and Giuraniuc, {Claudiu V.}",

note = "Acknowledgements We thank Marco Thiel and Alessandro De Moura for helpful discussions and advice, Stefan Hoppler and Mamen Romano for critical reading of the manuscript, James Hislop for his help with the plasma cleaner, Alex Brand for the microscopy imaging system and Alistair Robertson for fabricating the hydrostatic flow controllers. We also thank Diane Massie and Yvonne Turnbull for technical assistance. This work was supported by Scottish Universities Life Sciences Alliance (SULSA)and the University of Aberdeen .",

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doi = "10.1016/j.physa.2016.11.134",

language = "English",

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journal = "Physica. A, Statistical Mechanics and its Applications",

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T1 - Generation and precise control of dynamic biochemical gradients for cellular assays

AU - Saka, Yasushi

AU - MacPherson, Murray

AU - Giuraniuc, Claudiu V.

N1 - Acknowledgements We thank Marco Thiel and Alessandro De Moura for helpful discussions and advice, Stefan Hoppler and Mamen Romano for critical reading of the manuscript, James Hislop for his help with the plasma cleaner, Alex Brand for the microscopy imaging system and Alistair Robertson for fabricating the hydrostatic flow controllers. We also thank Diane Massie and Yvonne Turnbull for technical assistance. This work was supported by Scottish Universities Life Sciences Alliance (SULSA)and the University of Aberdeen .

PY - 2017/3/15

Y1 - 2017/3/15

N2 - Spatial gradients of diffusible signalling molecules play crucial roles in controlling diverse cellular behaviour such as cell differentiation, tissue patterning and chemotaxis. In this paper, we report the design and testing of a microfluidic device for diffusionbased gradient generation for cellular assays. A unique channel design of the device eliminates cross-flow between the source and sink channels, thereby stabilising gradients by passive diffusion. The platform also enables quick and flexible control of chemical concentration that makes highly dynamic gradients in diffusion chambers. A model with the first approximation of diffusion and surface adsorption of molecules recapitulates the experimentally observed gradients. Budding yeast cells cultured in a gradient of a chemical inducer expressed a reporter fluorescence protein in a concentration dependent manner. This microfluidic platform serves as a versatile prototype applicable to a broad range of biomedical investigations.

AB - Spatial gradients of diffusible signalling molecules play crucial roles in controlling diverse cellular behaviour such as cell differentiation, tissue patterning and chemotaxis. In this paper, we report the design and testing of a microfluidic device for diffusionbased gradient generation for cellular assays. A unique channel design of the device eliminates cross-flow between the source and sink channels, thereby stabilising gradients by passive diffusion. The platform also enables quick and flexible control of chemical concentration that makes highly dynamic gradients in diffusion chambers. A model with the first approximation of diffusion and surface adsorption of molecules recapitulates the experimentally observed gradients. Budding yeast cells cultured in a gradient of a chemical inducer expressed a reporter fluorescence protein in a concentration dependent manner. This microfluidic platform serves as a versatile prototype applicable to a broad range of biomedical investigations.

KW - Microfluidics

KW - Gradient

KW - Diffusion

KW - Yeast

KW - Saccharomyces cerevisiae

U2 - 10.1016/j.physa.2016.11.134

DO - 10.1016/j.physa.2016.11.134

M3 - Article

VL - 470

SP - 132

EP - 145

JO - Physica. A, Statistical Mechanics and its Applications

JF - Physica. A, Statistical Mechanics and its Applications

SN - 0378-4371

ER -

Generation and precise control of dynamic biochemical gradients for cellular assays

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Vasile Giuraniuc

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