Generation of chemiluminescence during enzymatic isolation of protoplasts from leaves of Oryza sativa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The isolation of protoplasts from basal leaf segments of rice (Oryza sativa) was associated with a dramatic increase in chemiluminescence, which may be interpreted as a measure of cellular active oxygen. This chemiluminescence emission was sensitive to inhibition by catalase (EC 1.11.1.6), hydroquinone, ascorbic acid and azide but: not by superoxide dismutase (EC 1.15.1.1) or 1,4,diazabicyclo [2.2.2.] octane. Peroxidase (EC 1.11.1.7) activity increased in the protoplasting solution during tissue digestion. The incubation of isolated cell walls in protoplasting solution also resulted in a stimulation of chemiluminescence which was again associated with elevated levels of peroxidase and hydrogen peroxide. We conclude that protoplast isolation in rice is associated with an increase in active oxygen species as measured by the increased chemiluminescence emission which results, in part, from the action of a peroxidase/hydrogen peroxide system.

Original languageEnglish
Pages (from-to)729-733
Number of pages5
JournalJournal of Plant Physiology
Volume150
Issue number6
Publication statusPublished - May 1997

Keywords

  • Oryza sativa
  • active oxygen
  • chemiluminescence
  • peroxidase
  • protoplast
  • rice
  • oxidative burst
  • chemi-luminescence
  • plant-cells
  • culture
  • regeneration
  • stimulation
  • involvement
  • toxicity

Cite this

Generation of chemiluminescence during enzymatic isolation of protoplasts from leaves of Oryza sativa. / Butt, A D ; Bestwick, Charles.

In: Journal of Plant Physiology, Vol. 150, No. 6, 05.1997, p. 729-733.

Research output: Contribution to journalArticle

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abstract = "The isolation of protoplasts from basal leaf segments of rice (Oryza sativa) was associated with a dramatic increase in chemiluminescence, which may be interpreted as a measure of cellular active oxygen. This chemiluminescence emission was sensitive to inhibition by catalase (EC 1.11.1.6), hydroquinone, ascorbic acid and azide but: not by superoxide dismutase (EC 1.15.1.1) or 1,4,diazabicyclo [2.2.2.] octane. Peroxidase (EC 1.11.1.7) activity increased in the protoplasting solution during tissue digestion. The incubation of isolated cell walls in protoplasting solution also resulted in a stimulation of chemiluminescence which was again associated with elevated levels of peroxidase and hydrogen peroxide. We conclude that protoplast isolation in rice is associated with an increase in active oxygen species as measured by the increased chemiluminescence emission which results, in part, from the action of a peroxidase/hydrogen peroxide system.",
keywords = "Oryza sativa, active oxygen, chemiluminescence, peroxidase, protoplast, rice, oxidative burst, chemi-luminescence, plant-cells, culture, regeneration, stimulation, involvement, toxicity",
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N2 - The isolation of protoplasts from basal leaf segments of rice (Oryza sativa) was associated with a dramatic increase in chemiluminescence, which may be interpreted as a measure of cellular active oxygen. This chemiluminescence emission was sensitive to inhibition by catalase (EC 1.11.1.6), hydroquinone, ascorbic acid and azide but: not by superoxide dismutase (EC 1.15.1.1) or 1,4,diazabicyclo [2.2.2.] octane. Peroxidase (EC 1.11.1.7) activity increased in the protoplasting solution during tissue digestion. The incubation of isolated cell walls in protoplasting solution also resulted in a stimulation of chemiluminescence which was again associated with elevated levels of peroxidase and hydrogen peroxide. We conclude that protoplast isolation in rice is associated with an increase in active oxygen species as measured by the increased chemiluminescence emission which results, in part, from the action of a peroxidase/hydrogen peroxide system.

AB - The isolation of protoplasts from basal leaf segments of rice (Oryza sativa) was associated with a dramatic increase in chemiluminescence, which may be interpreted as a measure of cellular active oxygen. This chemiluminescence emission was sensitive to inhibition by catalase (EC 1.11.1.6), hydroquinone, ascorbic acid and azide but: not by superoxide dismutase (EC 1.15.1.1) or 1,4,diazabicyclo [2.2.2.] octane. Peroxidase (EC 1.11.1.7) activity increased in the protoplasting solution during tissue digestion. The incubation of isolated cell walls in protoplasting solution also resulted in a stimulation of chemiluminescence which was again associated with elevated levels of peroxidase and hydrogen peroxide. We conclude that protoplast isolation in rice is associated with an increase in active oxygen species as measured by the increased chemiluminescence emission which results, in part, from the action of a peroxidase/hydrogen peroxide system.

KW - Oryza sativa

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KW - peroxidase

KW - protoplast

KW - rice

KW - oxidative burst

KW - chemi-luminescence

KW - plant-cells

KW - culture

KW - regeneration

KW - stimulation

KW - involvement

KW - toxicity

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EP - 733

JO - Journal of Plant Physiology

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