Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro

Carylyn J Marek, Gary A Cameron, Lucy J Elrick, Gabrielle M Hawksworth, Matthew C Wright

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.

Original languageEnglish
Pages (from-to)763-769
Number of pages7
JournalBiochemical Journal
Volume370
Issue number3
DOIs
Publication statusPublished - 15 Mar 2003

Keywords

  • Animals
  • Cell Differentiation
  • Cell Line
  • Cytochrome P-450 Enzyme System
  • Dexamethasone
  • Glucocorticoids
  • Hepatocytes
  • Male
  • Pancreas
  • Phenotype
  • Rats
  • Rats, Sprague-Dawley
  • Testosterone

Cite this

Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro. / Marek, Carylyn J; Cameron, Gary A; Elrick, Lucy J; Hawksworth, Gabrielle M; Wright, Matthew C.

In: Biochemical Journal, Vol. 370, No. 3, 15.03.2003, p. 763-769.

Research output: Contribution to journalArticle

Marek, Carylyn J ; Cameron, Gary A ; Elrick, Lucy J ; Hawksworth, Gabrielle M ; Wright, Matthew C. / Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro. In: Biochemical Journal. 2003 ; Vol. 370, No. 3. pp. 763-769.
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AU - Wright, Matthew C

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KW - Phenotype

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