Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro

Carylyn J Marek, Gary A Cameron, Lucy J Elrick, Gabrielle M Hawksworth, Matthew C Wright

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43 Citations (Scopus)

Abstract

The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.

Original languageEnglish
Pages (from-to)763-769
Number of pages7
JournalBiochemical Journal
Volume370
Issue number3
DOIs
Publication statusPublished - 15 Mar 2003

Keywords

  • Animals
  • Cell Differentiation
  • Cell Line
  • Cytochrome P-450 Enzyme System
  • Dexamethasone
  • Glucocorticoids
  • Hepatocytes
  • Male
  • Pancreas
  • Phenotype
  • Rats
  • Rats, Sprague-Dawley
  • Testosterone

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