Generation of human osteoclasts from peripheral blood

Kim Henriksen, Morten A Karsdal, Adam Taylor, Denise Tosh, Fraser Coxon

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Osteoclasts are multi-nucleated cells that have the unique ability to resorb calcified bone matrix. They derive from haematopoietic precursor cells, and can be generated in vitro by stimulation of peripheral blood mononuclear cells with the cytokines M-CSF and RANKL. In this chapter, we describe the method for generating human osteoclast from peripheral blood or buffy coats, as well as methods for studying both the differentiation and resorbing activity of these cells.

Original languageEnglish
Pages (from-to)159-175
Number of pages17
JournalMethods in Molecular Biology
Volume816
Early online date14 Oct 2011
DOIs
Publication statusPublished - 2012

Keywords

  • acid phosphatase
  • antigens, CD14
  • blood buffy coat
  • bone resorption
  • cell culture techniques
  • cell differentiation
  • collagen
  • cryopreservation
  • humans
  • integrin alphaVbeta3
  • isoenzymes
  • leukocytes, mononuclear
  • macrophage colony-stimulating factor
  • macrophages
  • osteoclasts
  • osteogenesis
  • RANK ligand

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    Henriksen, K., Karsdal, M. A., Taylor, A., Tosh, D., & Coxon, F. (2012). Generation of human osteoclasts from peripheral blood. Methods in Molecular Biology, 816, 159-175. https://doi.org/10.1007/978-1-61779-415-5_11