Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 714-727 |
| Number of pages | 14 |
| Journal | Genes, Brain, and Behavior |
| Volume | 8 |
| Issue number | 7 |
| Early online date | 23 Jun 2009 |
| DOIs | |
| Publication status | Published - Oct 2009 |
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Keywords
- age factors
- aging
- animals
- behavior, animal
- chromosome mapping
- chromosomes, mammalian
- crosses, genetic
- DNA mutational analysis
- epistasis, genetic
- female
- gene expression regulation, developmental
- genetic variation
- genotype
- male
- mice
- mice, inbred C57BL
- mice, inbred DBA
- mice, inbred strains
- motor skills
- penetrance
- phenotype
- quantitative trait loci
- sex factors
- species specificity
- time factors
- squares
- activity
- life span
- QTL
- rears
Cite this
Genetic architecture for hole-board behaviors across substantial time intervals in young, middle-aged and old mice. / Foreman, J E; Lionikas, A; Lang, D H; Gyekis, J P; Krishnan, M; Sharkey, N A; Gerhard, G S; Grant, M.D.; Vogler, G P; Mack, H.A.; Stout, J T; Griffith, J.W.; Lakoski, J M; Hofer, S M; McClearn, G E; Vandenbergh, D J; Blizard, D A.
In: Genes, Brain, and Behavior, Vol. 8, No. 7, 10.2009, p. 714-727.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Genetic architecture for hole-board behaviors across substantial time intervals in young, middle-aged and old mice
AU - Foreman, J E
AU - Lionikas, A
AU - Lang, D H
AU - Gyekis, J P
AU - Krishnan, M
AU - Sharkey, N A
AU - Gerhard, G S
AU - Grant, M.D.
AU - Vogler, G P
AU - Mack, H.A.
AU - Stout, J T
AU - Griffith, J.W.
AU - Lakoski, J M
AU - Hofer, S M
AU - McClearn, G E
AU - Vandenbergh, D J
AU - Blizard, D A
PY - 2009/10
Y1 - 2009/10
N2 - A quantitative trait locus (QTL) analysis of behaviors across the life span was conducted in F(2) mice from a C57BL/6J x DBA/2J cross and 22 BXD recombinant inbred (RI) strains. Mice of three age groups were tested in a hole-board apparatus for 3 min on three occasions approximately 1 month apart (average age at test 150, 450 and 750 days, approximately 400 mice per group, divided equally by sex). Quantitative trait loci with small effect size were found on 11 chromosomes for hole-board activity (Hbact) and hole-board rearing (Hbrear). Analysis of 22 RI strains tested at 150 and 450 days of age found only suggestive linkage, with four QTL for Hbact overlapping with those from the F(2) analysis. There was a significant phenotypic correlation between Hbact and Hbrear (approximately 0.55-0.69) and substantial commonality among QTL for the two behaviors. QTL analyses of head-pokes (HP) and fecal boli (FB) only identified QTL at the suggestive level of significance. Age accounted for approximately 15% of the phenotypic variance (sex approximately 3%), and there were genotype by age interactions at approximately 25% of the Hbact and Hbrear QTL. Quantitative trait loci for Hbrear were relatively stable across the three measurement occasions (those for Hbact somewhat less so), although mean levels of each index declined markedly comparing the first to subsequent trials. Considered as a whole, the polygenic system influencing exploratory behaviors accounts for approximately the same amount of phenotypic variance as age (within the range studied), is stable across substantial periods of time, and acts, for the most part, independently of age and sex.
AB - A quantitative trait locus (QTL) analysis of behaviors across the life span was conducted in F(2) mice from a C57BL/6J x DBA/2J cross and 22 BXD recombinant inbred (RI) strains. Mice of three age groups were tested in a hole-board apparatus for 3 min on three occasions approximately 1 month apart (average age at test 150, 450 and 750 days, approximately 400 mice per group, divided equally by sex). Quantitative trait loci with small effect size were found on 11 chromosomes for hole-board activity (Hbact) and hole-board rearing (Hbrear). Analysis of 22 RI strains tested at 150 and 450 days of age found only suggestive linkage, with four QTL for Hbact overlapping with those from the F(2) analysis. There was a significant phenotypic correlation between Hbact and Hbrear (approximately 0.55-0.69) and substantial commonality among QTL for the two behaviors. QTL analyses of head-pokes (HP) and fecal boli (FB) only identified QTL at the suggestive level of significance. Age accounted for approximately 15% of the phenotypic variance (sex approximately 3%), and there were genotype by age interactions at approximately 25% of the Hbact and Hbrear QTL. Quantitative trait loci for Hbrear were relatively stable across the three measurement occasions (those for Hbact somewhat less so), although mean levels of each index declined markedly comparing the first to subsequent trials. Considered as a whole, the polygenic system influencing exploratory behaviors accounts for approximately the same amount of phenotypic variance as age (within the range studied), is stable across substantial periods of time, and acts, for the most part, independently of age and sex.
KW - age factors
KW - aging
KW - animals
KW - behavior, animal
KW - chromosome mapping
KW - chromosomes, mammalian
KW - crosses, genetic
KW - DNA mutational analysis
KW - epistasis, genetic
KW - female
KW - gene expression regulation, developmental
KW - genetic variation
KW - genotype
KW - male
KW - mice
KW - mice, inbred C57BL
KW - mice, inbred DBA
KW - mice, inbred strains
KW - motor skills
KW - penetrance
KW - phenotype
KW - quantitative trait loci
KW - sex factors
KW - species specificity
KW - time factors
KW - squares
KW - activity
KW - life span
KW - QTL
KW - rears
U2 - 10.1111/j.1601-183X.2009.00516.x
DO - 10.1111/j.1601-183X.2009.00516.x
M3 - Article
VL - 8
SP - 714
EP - 727
JO - Genes, Brain, and Behavior
JF - Genes, Brain, and Behavior
SN - 1601-1848
IS - 7
ER -