Genetic association and characterization of FSTL5 in isolated clubfoot

Anas M Khanshour, Yared Kidane, Julia Kozlitina, Reuel Cornelia, Alexandra Rafipay, Vanessa De Mello, Mitchell Weston, Nandina Paria, Aysha Khalid, Jacqueline T Hecht, Matthew B Dobbs, B. Stephens Richards, Neil Vargesson, F Kent Hamra, Megan Wilson, Carol Wise, Christina A Gurnett, Jonathan J Rios* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Talipes equinovarus (clubfoot, TEV) is a congenital rotational foot deformity
occurring in 1 per 1,000 births with increased prevalence in males compared to females. The genetic etiology of isolated clubfoot (iTEV) remains unclear. Using a genome-wide association study, we identified a locus within FSTL5, encoding follistatin-like 5, significantly associated with iTEV. FSTL5 is an uncharacterized gene whose potential role in embryonic and post-natal development were previously unstudied. Utilizing multiple model systems, we found that Fstl5 was expressed during later stages of embryonic hindlimb development, and, in mice, expression was restricted to the condensing cartilage anlage destined to form the limb skeleton. In the post-natal growth plate, Fstl5 was specifically expressed in pre-hypertrophic chondrocytes. As Fstl5 knock out rats displayed no gross malformations, we engineered a conditional transgenic mouse
line (Fstl5LSL 54 ) to over-express Fstl5 in skeletal osteochondroprogenitors. We observed that hindlimbs were slightly shorter and that bone mineral density was reduced in adult male, but not female, Prrx1-cre;Fstl5LSL 56 mice compared to control. No overt clubfootlike deformity was observed in Prrx1-cre;Fstl5LSL 57 mice, suggesting FSTL5 may function in other cell types to contribute to iTEV pathogenesis. Interrogating published mouse embryonic single-cell expression data showed Fstl5 was expressed in cell lineage sub60 clusters whose transcriptomes were associated with neural system development. Moreover, our results suggest lineage-specific expression of the Fstl genes correlates with their divergent roles as modulators of TGF-ß and BMP signaling. Results from this study associate FSTL5 with iTEV and suggest an potential sexually dimorphic role for Fstl5 in vivo.
Original languageEnglish
JournalHuman Molecular Genetics
Publication statusAccepted/In press - 14 Oct 2020

Keywords

  • genome-wide association study
  • clubfoot
  • FSTL5
  • imputation

Fingerprint Dive into the research topics of 'Genetic association and characterization of FSTL5 in isolated clubfoot'. Together they form a unique fingerprint.

Cite this