Genetic diversity affects seedling survival but not growth or seed germination in the Bornean endemic dipterocarp Parashorea tomentella

Kirsty S. Nutt*, David F R P Burslem, Colin R. Maycock, Jaboury Ghazoul, Eyen Khoo, Alexander Y L Hastie, Chris J. Kettle

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)
5 Downloads (Pure)

Abstract

Background: Logging and habitat fragmentation of tropical rain forests may disrupt patterns of gene flow and genetic diversity. Consequently, inbreeding in tree populations may reduce fitness and increase extinction risks, especially among species that are predominantly outcrossing, dependent on biotic pollination and/or display limited seed dispersal such as species of the Dipterocarpaceae. Aims: To test the hypothesis that heterozygosity of individual progeny affects their likelihood of germination and the growth and survival of seedlings. Methods: Standardised measure of multilocus heterozygosity (sMLH) was estimated from seven microsatellite loci for individual progeny collected from 18 mother trees of the large dipterocarp Parashorea tomentella. The relationships among sMLH, germination and seedling growth and survival were determined for the progeny. Results: Seedling survival over 18 months increased with greater sMLH and fresh fruit weight. This result was expressed under all experimentally controlled combinations of light and nutrient availability in the nursery and in the shaded understorey of primary forest where survival overall was much lower than in the nursery. sMLH did not affect the probability of germination or seedling growth rate in any experimental treatment. Conclusions: These results provide evidence that reduced heterozygosity is associated with reduced seedling survival in a tropical forest tree species.

Original languageEnglish
Pages (from-to)471-481
Number of pages11
JournalPlant Ecology & Diversity
Volume9
Issue number5-6
DOIs
Publication statusPublished - 2016

Fingerprint

Parashorea
Tomentella
Dipterocarpaceae
heterozygosity
germination
seed germination
seedling
seed
genetic variation
seedlings
seedling growth
extinction risk
seed trees
outcrossing
primary forests
light availability
habitat fragmentation
tropical rain forests
seed dispersal
inbreeding

Keywords

  • Dipterocarpaceae
  • fitness
  • genetic diversity
  • heterozygosity
  • inbreeding depression
  • seedling survival
  • sustainable logging

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science

Cite this

Genetic diversity affects seedling survival but not growth or seed germination in the Bornean endemic dipterocarp Parashorea tomentella. / Nutt, Kirsty S.; Burslem, David F R P; Maycock, Colin R.; Ghazoul, Jaboury; Khoo, Eyen; Hastie, Alexander Y L; Kettle, Chris J.

In: Plant Ecology & Diversity, Vol. 9, No. 5-6, 2016, p. 471-481.

Research output: Contribution to journalArticle

Nutt, Kirsty S. ; Burslem, David F R P ; Maycock, Colin R. ; Ghazoul, Jaboury ; Khoo, Eyen ; Hastie, Alexander Y L ; Kettle, Chris J. / Genetic diversity affects seedling survival but not growth or seed germination in the Bornean endemic dipterocarp Parashorea tomentella. In: Plant Ecology & Diversity. 2016 ; Vol. 9, No. 5-6. pp. 471-481.
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abstract = "Background: Logging and habitat fragmentation of tropical rain forests may disrupt patterns of gene flow and genetic diversity. Consequently, inbreeding in tree populations may reduce fitness and increase extinction risks, especially among species that are predominantly outcrossing, dependent on biotic pollination and/or display limited seed dispersal such as species of the Dipterocarpaceae. Aims: To test the hypothesis that heterozygosity of individual progeny affects their likelihood of germination and the growth and survival of seedlings. Methods: Standardised measure of multilocus heterozygosity (sMLH) was estimated from seven microsatellite loci for individual progeny collected from 18 mother trees of the large dipterocarp Parashorea tomentella. The relationships among sMLH, germination and seedling growth and survival were determined for the progeny. Results: Seedling survival over 18 months increased with greater sMLH and fresh fruit weight. This result was expressed under all experimentally controlled combinations of light and nutrient availability in the nursery and in the shaded understorey of primary forest where survival overall was much lower than in the nursery. sMLH did not affect the probability of germination or seedling growth rate in any experimental treatment. Conclusions: These results provide evidence that reduced heterozygosity is associated with reduced seedling survival in a tropical forest tree species.",
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note = "Acknowledgements We thank the Sabah State Government for permission to conduct research in Sabah. We also thank Anis and Jeisin and particularly Maricel Verancis for research assistance and colleagues at the Forest Research Centre for providing equipment, facilities, and advice. DNA extractions were undertaken at Forest Research Centre, Malaysia, and at the Genetic Diversity Centre of ETH Zurich where fragment analysis was conducted. The research was funded by the UK’s Natural Environment Research Council with additional support from the University of Aberdeen’s Principal’s Fund, the Sutherland Bequest and the Society for Experimental Biology. Published online: 04 Jan 2017",
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T1 - Genetic diversity affects seedling survival but not growth or seed germination in the Bornean endemic dipterocarp Parashorea tomentella

AU - Nutt, Kirsty S.

AU - Burslem, David F R P

AU - Maycock, Colin R.

AU - Ghazoul, Jaboury

AU - Khoo, Eyen

AU - Hastie, Alexander Y L

AU - Kettle, Chris J.

N1 - Acknowledgements We thank the Sabah State Government for permission to conduct research in Sabah. We also thank Anis and Jeisin and particularly Maricel Verancis for research assistance and colleagues at the Forest Research Centre for providing equipment, facilities, and advice. DNA extractions were undertaken at Forest Research Centre, Malaysia, and at the Genetic Diversity Centre of ETH Zurich where fragment analysis was conducted. The research was funded by the UK’s Natural Environment Research Council with additional support from the University of Aberdeen’s Principal’s Fund, the Sutherland Bequest and the Society for Experimental Biology. Published online: 04 Jan 2017

PY - 2016

Y1 - 2016

N2 - Background: Logging and habitat fragmentation of tropical rain forests may disrupt patterns of gene flow and genetic diversity. Consequently, inbreeding in tree populations may reduce fitness and increase extinction risks, especially among species that are predominantly outcrossing, dependent on biotic pollination and/or display limited seed dispersal such as species of the Dipterocarpaceae. Aims: To test the hypothesis that heterozygosity of individual progeny affects their likelihood of germination and the growth and survival of seedlings. Methods: Standardised measure of multilocus heterozygosity (sMLH) was estimated from seven microsatellite loci for individual progeny collected from 18 mother trees of the large dipterocarp Parashorea tomentella. The relationships among sMLH, germination and seedling growth and survival were determined for the progeny. Results: Seedling survival over 18 months increased with greater sMLH and fresh fruit weight. This result was expressed under all experimentally controlled combinations of light and nutrient availability in the nursery and in the shaded understorey of primary forest where survival overall was much lower than in the nursery. sMLH did not affect the probability of germination or seedling growth rate in any experimental treatment. Conclusions: These results provide evidence that reduced heterozygosity is associated with reduced seedling survival in a tropical forest tree species.

AB - Background: Logging and habitat fragmentation of tropical rain forests may disrupt patterns of gene flow and genetic diversity. Consequently, inbreeding in tree populations may reduce fitness and increase extinction risks, especially among species that are predominantly outcrossing, dependent on biotic pollination and/or display limited seed dispersal such as species of the Dipterocarpaceae. Aims: To test the hypothesis that heterozygosity of individual progeny affects their likelihood of germination and the growth and survival of seedlings. Methods: Standardised measure of multilocus heterozygosity (sMLH) was estimated from seven microsatellite loci for individual progeny collected from 18 mother trees of the large dipterocarp Parashorea tomentella. The relationships among sMLH, germination and seedling growth and survival were determined for the progeny. Results: Seedling survival over 18 months increased with greater sMLH and fresh fruit weight. This result was expressed under all experimentally controlled combinations of light and nutrient availability in the nursery and in the shaded understorey of primary forest where survival overall was much lower than in the nursery. sMLH did not affect the probability of germination or seedling growth rate in any experimental treatment. Conclusions: These results provide evidence that reduced heterozygosity is associated with reduced seedling survival in a tropical forest tree species.

KW - Dipterocarpaceae

KW - fitness

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KW - heterozygosity

KW - inbreeding depression

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