Genetic polymorphism in ornithine decarboxylase and risk of breast cancer

Iain Brown, Susan Halliday, Heather Greig, Steven D Heys, Heather M Wallace, Andrew C Schofield

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ornithine decarboxylase (ODC), the first enzyme in the biosynthesis of polyamines, has increased activity in breast cancer tissue compared with benign and normal tissues. The ODC gene contains a single nucleotide polymorphism in which a guanine is substituted for an adenine. This study investigated whether the ODC +316 G > A polymorphism (rs2302615) was associated with the risk of developing breast cancer. A case–control study involving 121 controls, without breast cancer, 46 patients with breast cancer but without a family history, and 130 breast cancer cases with a family history of breast cancer was conducted. A nested PCR-restriction fragment length polymorphism procedure and the TaqMan 5' nuclease assay was used to genotype individuals. Risk was significantly lower for heterozygote (GA genotype) individuals [odds ratio (OR) = 0.39, 95% confidence interval (CI) 0.17–0.86, P = 0.018], or individuals with at least one A allele (OR = 0.44, 95% CI 0.21–0.92, P = 0.027), without family history. This protective effect of having at least one copy of the variant A allele was not as strong, however, in those with a family history of the disease. In sporadic breast cancer, the presence of at least one A allele is protective against the disease. The influence of this polymorphism may be less important in individuals with an inherited breast cancer predisposition.
Original languageEnglish
Pages (from-to)307-311
Number of pages5
JournalFamilial Cancer
Volume8
Issue number4
Early online date19 Feb 2009
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Ornithine Decarboxylase
Genetic Polymorphisms
Breast Neoplasms
Alleles
Odds Ratio
Genotype
Confidence Intervals
Polyamines
Guanine
Adenine
Heterozygote
Restriction Fragment Length Polymorphisms
Single Nucleotide Polymorphism
Polymerase Chain Reaction
Enzymes

Keywords

  • breast cancer
  • case-control study
  • ODC
  • polyamine
  • gene polymorphism

Cite this

Genetic polymorphism in ornithine decarboxylase and risk of breast cancer. / Brown, Iain; Halliday, Susan; Greig, Heather; Heys, Steven D; Wallace, Heather M; Schofield, Andrew C.

In: Familial Cancer, Vol. 8, No. 4, 12.2009, p. 307-311.

Research output: Contribution to journalArticle

Brown, I, Halliday, S, Greig, H, Heys, SD, Wallace, HM & Schofield, AC 2009, 'Genetic polymorphism in ornithine decarboxylase and risk of breast cancer', Familial Cancer, vol. 8, no. 4, pp. 307-311. https://doi.org/10.1007/s10689-009-9237-9
Brown, Iain ; Halliday, Susan ; Greig, Heather ; Heys, Steven D ; Wallace, Heather M ; Schofield, Andrew C. / Genetic polymorphism in ornithine decarboxylase and risk of breast cancer. In: Familial Cancer. 2009 ; Vol. 8, No. 4. pp. 307-311.
@article{f1b4a9c804cf4854a7eb70789e21b9e2,
title = "Genetic polymorphism in ornithine decarboxylase and risk of breast cancer",
abstract = "Ornithine decarboxylase (ODC), the first enzyme in the biosynthesis of polyamines, has increased activity in breast cancer tissue compared with benign and normal tissues. The ODC gene contains a single nucleotide polymorphism in which a guanine is substituted for an adenine. This study investigated whether the ODC +316 G > A polymorphism (rs2302615) was associated with the risk of developing breast cancer. A case–control study involving 121 controls, without breast cancer, 46 patients with breast cancer but without a family history, and 130 breast cancer cases with a family history of breast cancer was conducted. A nested PCR-restriction fragment length polymorphism procedure and the TaqMan 5' nuclease assay was used to genotype individuals. Risk was significantly lower for heterozygote (GA genotype) individuals [odds ratio (OR) = 0.39, 95{\%} confidence interval (CI) 0.17–0.86, P = 0.018], or individuals with at least one A allele (OR = 0.44, 95{\%} CI 0.21–0.92, P = 0.027), without family history. This protective effect of having at least one copy of the variant A allele was not as strong, however, in those with a family history of the disease. In sporadic breast cancer, the presence of at least one A allele is protective against the disease. The influence of this polymorphism may be less important in individuals with an inherited breast cancer predisposition.",
keywords = "breast cancer, case-control study, ODC , polyamine, gene polymorphism",
author = "Iain Brown and Susan Halliday and Heather Greig and Heys, {Steven D} and Wallace, {Heather M} and Schofield, {Andrew C}",
year = "2009",
month = "12",
doi = "10.1007/s10689-009-9237-9",
language = "English",
volume = "8",
pages = "307--311",
journal = "Familial Cancer",
issn = "1389-9600",
publisher = "Springer Netherlands",
number = "4",

}

TY - JOUR

T1 - Genetic polymorphism in ornithine decarboxylase and risk of breast cancer

AU - Brown, Iain

AU - Halliday, Susan

AU - Greig, Heather

AU - Heys, Steven D

AU - Wallace, Heather M

AU - Schofield, Andrew C

PY - 2009/12

Y1 - 2009/12

N2 - Ornithine decarboxylase (ODC), the first enzyme in the biosynthesis of polyamines, has increased activity in breast cancer tissue compared with benign and normal tissues. The ODC gene contains a single nucleotide polymorphism in which a guanine is substituted for an adenine. This study investigated whether the ODC +316 G > A polymorphism (rs2302615) was associated with the risk of developing breast cancer. A case–control study involving 121 controls, without breast cancer, 46 patients with breast cancer but without a family history, and 130 breast cancer cases with a family history of breast cancer was conducted. A nested PCR-restriction fragment length polymorphism procedure and the TaqMan 5' nuclease assay was used to genotype individuals. Risk was significantly lower for heterozygote (GA genotype) individuals [odds ratio (OR) = 0.39, 95% confidence interval (CI) 0.17–0.86, P = 0.018], or individuals with at least one A allele (OR = 0.44, 95% CI 0.21–0.92, P = 0.027), without family history. This protective effect of having at least one copy of the variant A allele was not as strong, however, in those with a family history of the disease. In sporadic breast cancer, the presence of at least one A allele is protective against the disease. The influence of this polymorphism may be less important in individuals with an inherited breast cancer predisposition.

AB - Ornithine decarboxylase (ODC), the first enzyme in the biosynthesis of polyamines, has increased activity in breast cancer tissue compared with benign and normal tissues. The ODC gene contains a single nucleotide polymorphism in which a guanine is substituted for an adenine. This study investigated whether the ODC +316 G > A polymorphism (rs2302615) was associated with the risk of developing breast cancer. A case–control study involving 121 controls, without breast cancer, 46 patients with breast cancer but without a family history, and 130 breast cancer cases with a family history of breast cancer was conducted. A nested PCR-restriction fragment length polymorphism procedure and the TaqMan 5' nuclease assay was used to genotype individuals. Risk was significantly lower for heterozygote (GA genotype) individuals [odds ratio (OR) = 0.39, 95% confidence interval (CI) 0.17–0.86, P = 0.018], or individuals with at least one A allele (OR = 0.44, 95% CI 0.21–0.92, P = 0.027), without family history. This protective effect of having at least one copy of the variant A allele was not as strong, however, in those with a family history of the disease. In sporadic breast cancer, the presence of at least one A allele is protective against the disease. The influence of this polymorphism may be less important in individuals with an inherited breast cancer predisposition.

KW - breast cancer

KW - case-control study

KW - ODC

KW - polyamine

KW - gene polymorphism

U2 - 10.1007/s10689-009-9237-9

DO - 10.1007/s10689-009-9237-9

M3 - Article

VL - 8

SP - 307

EP - 311

JO - Familial Cancer

JF - Familial Cancer

SN - 1389-9600

IS - 4

ER -