Genetic variants in selenoprotein genes modulate biomarkers of selenium status in response to Brazil nut supplementation (the SU.BRA.NUT study)

Janaina L.S. Donadio, Marcelo M. Rogero, Elvira M. Guerra-Shinohara, Fernando Barbosa Jr., Charles Desmarchelier, Patrick Borel, Alan Sneddon, John Hesketh, Silvia M.F. Cozzolino

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Abstract

SummaryBackground The beneficial effects of selenium (Se) to human health are exerted by selenoproteins, which can be quantified in blood and used as biomarkers of Se status. Different responses of Se biomarkers after supplementation with selenomethionine and sodium selenite have been observed and some of them could be due to genetic polymorphisms, mainly single nucleotide polymorphisms (SNPs). Brazil nuts are known to be the richest natural source of Se. Objective Investigate how genetic variations in selenoprotein genes modulate biomarkers of Se status in response to Brazil nut supplementation. Methods The SU.BRA.NUT study was a four month interventional trial which involved healthy volunteers of both genders, selected in University of Sao Paulo. The supplementation was done with one Brazil nut a day for 8 weeks, followed by 8 weeks of washout. Blood samples were collected at 5 time points: baseline, 4 and 8 weeks of supplementation and 4 and 8 weeks of washout for analysis of five biomarkers of Se status – erythrocyte GPx1 (Glutathione Peroxidase 1) activity, plasma GPx3 activity, plasma Se, erythrocyte Se, and plasma selenoprotein P. The gene expression of GPX1, SELENOP, SELENOF and SELENOS was done before and after 8 weeks of supplementation. The volunteers were genotyped for SNPs in GPX1 (rs1050450, rs3811699 and rs1800699), GPX4 (rs713041), SELENOP (rs3877899 and rs7579), SELENOF (rs5845) and SELENOS (rs34713741). Results A total of 130 volunteers finished the protocol. The concentrations of four biomarkers of Se status increased significantly after 4 and 8 weeks of supplementation, being modulated by gender. In addition, erythrocyte GPx1 activity was associated with rs1050450, rs713041 and rs5845. Plasma Se was associated with rs7579 and selenoprotein P with plasma Se at baseline. Nut supplementation significantly increased GPX1 mRNA expression only in subjects with CC genotype at rs1050450. SELENOP mRNA expression was significantly lower in subjects with GG genotype at rs7579 before and after supplementation. Conclusion Genetic variations in GPX1 and SELENOP genes are associated with different responses of molecular and biochemical biomarkers of Se status after Brazil nut supplementation in healthy Brazilians. The SU.BRA.NUT study was registred at www.clinicaltrials.gov as NCT 03111355.
Original languageEnglish
Pages (from-to)539-548
Number of pages10
JournalClinical Nutrition
Volume38
Issue number2
Early online date23 Mar 2018
DOIs
Publication statusPublished - Apr 2019

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Bertholletia
Selenoproteins
Selenium
Biomarkers
Genes
Selenoprotein P
Erythrocytes
Single Nucleotide Polymorphism
Volunteers
Genotype
Selenomethionine
Sodium Selenite
Messenger RNA
Nuts
Genetic Polymorphisms

Keywords

  • Glutathione peroxidase
  • SNPs
  • Selenium
  • Polymorphisms
  • Nutrigenetics

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Nutrition and Dietetics

Cite this

Donadio, J. L. S., Rogero, M. M., Guerra-Shinohara, E. M., Barbosa Jr., F., Desmarchelier, C., Borel, P., ... Cozzolino, S. M. F. (2019). Genetic variants in selenoprotein genes modulate biomarkers of selenium status in response to Brazil nut supplementation (the SU.BRA.NUT study). Clinical Nutrition, 38(2), 539-548. https://doi.org/10.1016/j.clnu.2018.03.011

Genetic variants in selenoprotein genes modulate biomarkers of selenium status in response to Brazil nut supplementation (the SU.BRA.NUT study). / Donadio, Janaina L.S.; Rogero, Marcelo M.; Guerra-Shinohara, Elvira M.; Barbosa Jr., Fernando; Desmarchelier, Charles; Borel, Patrick; Sneddon, Alan; Hesketh, John; Cozzolino, Silvia M.F.

In: Clinical Nutrition, Vol. 38, No. 2, 04.2019, p. 539-548.

Research output: Contribution to journalArticle

Donadio, JLS, Rogero, MM, Guerra-Shinohara, EM, Barbosa Jr., F, Desmarchelier, C, Borel, P, Sneddon, A, Hesketh, J & Cozzolino, SMF 2019, 'Genetic variants in selenoprotein genes modulate biomarkers of selenium status in response to Brazil nut supplementation (the SU.BRA.NUT study)' Clinical Nutrition, vol. 38, no. 2, pp. 539-548. https://doi.org/10.1016/j.clnu.2018.03.011
Donadio, Janaina L.S. ; Rogero, Marcelo M. ; Guerra-Shinohara, Elvira M. ; Barbosa Jr., Fernando ; Desmarchelier, Charles ; Borel, Patrick ; Sneddon, Alan ; Hesketh, John ; Cozzolino, Silvia M.F. / Genetic variants in selenoprotein genes modulate biomarkers of selenium status in response to Brazil nut supplementation (the SU.BRA.NUT study). In: Clinical Nutrition. 2019 ; Vol. 38, No. 2. pp. 539-548.
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title = "Genetic variants in selenoprotein genes modulate biomarkers of selenium status in response to Brazil nut supplementation (the SU.BRA.NUT study)",
abstract = "SummaryBackground The beneficial effects of selenium (Se) to human health are exerted by selenoproteins, which can be quantified in blood and used as biomarkers of Se status. Different responses of Se biomarkers after supplementation with selenomethionine and sodium selenite have been observed and some of them could be due to genetic polymorphisms, mainly single nucleotide polymorphisms (SNPs). Brazil nuts are known to be the richest natural source of Se. Objective Investigate how genetic variations in selenoprotein genes modulate biomarkers of Se status in response to Brazil nut supplementation. Methods The SU.BRA.NUT study was a four month interventional trial which involved healthy volunteers of both genders, selected in University of Sao Paulo. The supplementation was done with one Brazil nut a day for 8 weeks, followed by 8 weeks of washout. Blood samples were collected at 5 time points: baseline, 4 and 8 weeks of supplementation and 4 and 8 weeks of washout for analysis of five biomarkers of Se status – erythrocyte GPx1 (Glutathione Peroxidase 1) activity, plasma GPx3 activity, plasma Se, erythrocyte Se, and plasma selenoprotein P. The gene expression of GPX1, SELENOP, SELENOF and SELENOS was done before and after 8 weeks of supplementation. The volunteers were genotyped for SNPs in GPX1 (rs1050450, rs3811699 and rs1800699), GPX4 (rs713041), SELENOP (rs3877899 and rs7579), SELENOF (rs5845) and SELENOS (rs34713741). Results A total of 130 volunteers finished the protocol. The concentrations of four biomarkers of Se status increased significantly after 4 and 8 weeks of supplementation, being modulated by gender. In addition, erythrocyte GPx1 activity was associated with rs1050450, rs713041 and rs5845. Plasma Se was associated with rs7579 and selenoprotein P with plasma Se at baseline. Nut supplementation significantly increased GPX1 mRNA expression only in subjects with CC genotype at rs1050450. SELENOP mRNA expression was significantly lower in subjects with GG genotype at rs7579 before and after supplementation. Conclusion Genetic variations in GPX1 and SELENOP genes are associated with different responses of molecular and biochemical biomarkers of Se status after Brazil nut supplementation in healthy Brazilians. The SU.BRA.NUT study was registred at www.clinicaltrials.gov as NCT 03111355.",
keywords = "Glutathione peroxidase, SNPs, Selenium, Polymorphisms, Nutrigenetics",
author = "Donadio, {Janaina L.S.} and Rogero, {Marcelo M.} and Guerra-Shinohara, {Elvira M.} and {Barbosa Jr.}, Fernando and Charles Desmarchelier and Patrick Borel and Alan Sneddon and John Hesketh and Cozzolino, {Silvia M.F.}",
note = "This work was supported by Brazilian grants from S{\~a}o Paulo Research Foundation to JLSD (Funda{\cc}{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo-FAPESP processes: 2011/17720-0 and 2015/10146-8). Funding source had no involvement in study design, collection, analysis and interpretation of data from the present research.",
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doi = "10.1016/j.clnu.2018.03.011",
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T1 - Genetic variants in selenoprotein genes modulate biomarkers of selenium status in response to Brazil nut supplementation (the SU.BRA.NUT study)

AU - Donadio, Janaina L.S.

AU - Rogero, Marcelo M.

AU - Guerra-Shinohara, Elvira M.

AU - Barbosa Jr., Fernando

AU - Desmarchelier, Charles

AU - Borel, Patrick

AU - Sneddon, Alan

AU - Hesketh, John

AU - Cozzolino, Silvia M.F.

N1 - This work was supported by Brazilian grants from São Paulo Research Foundation to JLSD (Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP processes: 2011/17720-0 and 2015/10146-8). Funding source had no involvement in study design, collection, analysis and interpretation of data from the present research.

PY - 2019/4

Y1 - 2019/4

N2 - SummaryBackground The beneficial effects of selenium (Se) to human health are exerted by selenoproteins, which can be quantified in blood and used as biomarkers of Se status. Different responses of Se biomarkers after supplementation with selenomethionine and sodium selenite have been observed and some of them could be due to genetic polymorphisms, mainly single nucleotide polymorphisms (SNPs). Brazil nuts are known to be the richest natural source of Se. Objective Investigate how genetic variations in selenoprotein genes modulate biomarkers of Se status in response to Brazil nut supplementation. Methods The SU.BRA.NUT study was a four month interventional trial which involved healthy volunteers of both genders, selected in University of Sao Paulo. The supplementation was done with one Brazil nut a day for 8 weeks, followed by 8 weeks of washout. Blood samples were collected at 5 time points: baseline, 4 and 8 weeks of supplementation and 4 and 8 weeks of washout for analysis of five biomarkers of Se status – erythrocyte GPx1 (Glutathione Peroxidase 1) activity, plasma GPx3 activity, plasma Se, erythrocyte Se, and plasma selenoprotein P. The gene expression of GPX1, SELENOP, SELENOF and SELENOS was done before and after 8 weeks of supplementation. The volunteers were genotyped for SNPs in GPX1 (rs1050450, rs3811699 and rs1800699), GPX4 (rs713041), SELENOP (rs3877899 and rs7579), SELENOF (rs5845) and SELENOS (rs34713741). Results A total of 130 volunteers finished the protocol. The concentrations of four biomarkers of Se status increased significantly after 4 and 8 weeks of supplementation, being modulated by gender. In addition, erythrocyte GPx1 activity was associated with rs1050450, rs713041 and rs5845. Plasma Se was associated with rs7579 and selenoprotein P with plasma Se at baseline. Nut supplementation significantly increased GPX1 mRNA expression only in subjects with CC genotype at rs1050450. SELENOP mRNA expression was significantly lower in subjects with GG genotype at rs7579 before and after supplementation. Conclusion Genetic variations in GPX1 and SELENOP genes are associated with different responses of molecular and biochemical biomarkers of Se status after Brazil nut supplementation in healthy Brazilians. The SU.BRA.NUT study was registred at www.clinicaltrials.gov as NCT 03111355.

AB - SummaryBackground The beneficial effects of selenium (Se) to human health are exerted by selenoproteins, which can be quantified in blood and used as biomarkers of Se status. Different responses of Se biomarkers after supplementation with selenomethionine and sodium selenite have been observed and some of them could be due to genetic polymorphisms, mainly single nucleotide polymorphisms (SNPs). Brazil nuts are known to be the richest natural source of Se. Objective Investigate how genetic variations in selenoprotein genes modulate biomarkers of Se status in response to Brazil nut supplementation. Methods The SU.BRA.NUT study was a four month interventional trial which involved healthy volunteers of both genders, selected in University of Sao Paulo. The supplementation was done with one Brazil nut a day for 8 weeks, followed by 8 weeks of washout. Blood samples were collected at 5 time points: baseline, 4 and 8 weeks of supplementation and 4 and 8 weeks of washout for analysis of five biomarkers of Se status – erythrocyte GPx1 (Glutathione Peroxidase 1) activity, plasma GPx3 activity, plasma Se, erythrocyte Se, and plasma selenoprotein P. The gene expression of GPX1, SELENOP, SELENOF and SELENOS was done before and after 8 weeks of supplementation. The volunteers were genotyped for SNPs in GPX1 (rs1050450, rs3811699 and rs1800699), GPX4 (rs713041), SELENOP (rs3877899 and rs7579), SELENOF (rs5845) and SELENOS (rs34713741). Results A total of 130 volunteers finished the protocol. The concentrations of four biomarkers of Se status increased significantly after 4 and 8 weeks of supplementation, being modulated by gender. In addition, erythrocyte GPx1 activity was associated with rs1050450, rs713041 and rs5845. Plasma Se was associated with rs7579 and selenoprotein P with plasma Se at baseline. Nut supplementation significantly increased GPX1 mRNA expression only in subjects with CC genotype at rs1050450. SELENOP mRNA expression was significantly lower in subjects with GG genotype at rs7579 before and after supplementation. Conclusion Genetic variations in GPX1 and SELENOP genes are associated with different responses of molecular and biochemical biomarkers of Se status after Brazil nut supplementation in healthy Brazilians. The SU.BRA.NUT study was registred at www.clinicaltrials.gov as NCT 03111355.

KW - Glutathione peroxidase

KW - SNPs

KW - Selenium

KW - Polymorphisms

KW - Nutrigenetics

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