Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging

Francesca Turroni, Francesca Bottacini, Elena Foroni, Imke Mulder, Jae-Han Kim, Aldert Zomer, Borja Sánchez, Alessandro Bidossi, Alberto Ferrarini, Vanessa Giubellini, Massimo Delledonne, Bernard Henrissat, Pedro Coutinho, Marco Oggioni, Gerald F. Fitzgerald, David Mills, Abelardo Margolles, Denise Kelly, Douwe van Sinderen, Marco Ventura

Research output: Contribution to journalArticle

209 Citations (Scopus)

Abstract

The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling revealed a set of chromosomal loci responsible for mucin metabolism that appear to be under common transcriptional control and with predicted functions that allow degradation of various O-linked glycans in mucin. Conservation of the latter gene clusters in various B. bifidum strains supports the notion that host-derived glycan catabolism is an important colonization factor for B. bifidum with concomitant impact on intestinal microbiota ecology.
Original languageEnglish
Pages (from-to)19514-19519
Number of pages6
JournalPNAS
Volume107
Issue number45
Early online date25 Oct 2010
DOIs
Publication statusPublished - 9 Nov 2010

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Mucins
Metabolic Networks and Pathways
Polysaccharides
Bifidobacterium
Genome
Microbial Consortia
Gene Expression Profiling
Proteome
Multigene Family
Ecology
Intestines
Food
Bifidobacterium bifidum

Keywords

  • bifidobacterium
  • feces
  • gene expression profiling
  • genome, bacterial
  • genomics
  • host-pathogen interactions
  • humans
  • infant, newborn
  • intestines
  • metabolic networks and pathways
  • molecular sequence data
  • mucins
  • multigene family
  • polysaccharides

Cite this

Turroni, F., Bottacini, F., Foroni, E., Mulder, I., Kim, J-H., Zomer, A., ... Ventura, M. (2010). Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging. PNAS, 107(45), 19514-19519. https://doi.org/10.1073/pnas.1011100107

Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging. / Turroni, Francesca; Bottacini, Francesca; Foroni, Elena; Mulder, Imke; Kim, Jae-Han; Zomer, Aldert; Sánchez, Borja; Bidossi, Alessandro; Ferrarini, Alberto; Giubellini, Vanessa; Delledonne, Massimo; Henrissat, Bernard; Coutinho, Pedro; Oggioni, Marco; Fitzgerald, Gerald F.; Mills, David; Margolles, Abelardo; Kelly, Denise; van Sinderen, Douwe; Ventura, Marco.

In: PNAS, Vol. 107, No. 45, 09.11.2010, p. 19514-19519.

Research output: Contribution to journalArticle

Turroni, F, Bottacini, F, Foroni, E, Mulder, I, Kim, J-H, Zomer, A, Sánchez, B, Bidossi, A, Ferrarini, A, Giubellini, V, Delledonne, M, Henrissat, B, Coutinho, P, Oggioni, M, Fitzgerald, GF, Mills, D, Margolles, A, Kelly, D, van Sinderen, D & Ventura, M 2010, 'Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging', PNAS, vol. 107, no. 45, pp. 19514-19519. https://doi.org/10.1073/pnas.1011100107
Turroni F, Bottacini F, Foroni E, Mulder I, Kim J-H, Zomer A et al. Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging. PNAS. 2010 Nov 9;107(45):19514-19519. https://doi.org/10.1073/pnas.1011100107
Turroni, Francesca ; Bottacini, Francesca ; Foroni, Elena ; Mulder, Imke ; Kim, Jae-Han ; Zomer, Aldert ; Sánchez, Borja ; Bidossi, Alessandro ; Ferrarini, Alberto ; Giubellini, Vanessa ; Delledonne, Massimo ; Henrissat, Bernard ; Coutinho, Pedro ; Oggioni, Marco ; Fitzgerald, Gerald F. ; Mills, David ; Margolles, Abelardo ; Kelly, Denise ; van Sinderen, Douwe ; Ventura, Marco. / Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging. In: PNAS. 2010 ; Vol. 107, No. 45. pp. 19514-19519.
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