Genome engineering using site-specific recombinases

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    The targeted modification of the mammalian genome has a variety of applications in research, medicine, and biotechnology. Site-specific recombinases have become significant tools in all of these areas. Conditional gene targeting using site-specific recombinases has enabled the functional analysis of genes, which cannot be inactivated in the germline. The site-specific integration of adeno-associated virus, a major gene therapy vehicle, relies on the recombinase activity of the viral rep proteins. Site-specific recombinases also allow the precise integration of open reading frames encoding pharmaceutically relevant proteins into highly active gene loci in cell lines and transgenic animals. These goals have been accomplished by using a variety of genetic strategies but only a few recombinase proteins. However, the vast repertoire of recombinases, which has recently become available as a result of large-scale sequencing projects, may provide a rich source for the development of novel strategies to precisely alter mammalian genomes.
    Original languageEnglish
    Pages (from-to)65-80
    Number of pages16
    JournalCloning and Stem Cells
    Volume4
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 2002

    Keywords

    • Animals
    • Animals, Genetically Modified
    • DNA
    • DNA Nucleotidyltransferases
    • Dependovirus
    • Genetic Engineering
    • Genetic Techniques
    • Genetic Vectors
    • Humans
    • Mice
    • Models, Genetic
    • Mutagenesis, Site-Directed
    • Open Reading Frames
    • Recombinases

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