Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney

Louise A Walker; Donna M MacCallum; Gwyneth Bertram; Neil A R Gow; Frank C Odds; Alistair J P Brown

doi:10.1016/j.fgb.2008.10.012

Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney

Louise A Walker, Donna M MacCallum, Gwyneth Bertram, Neil A R Gow, Frank C Odds, Alistair J P Brown

Medical Sciences

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Abstract

Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.

Original language	English
Pages (from-to)	210-219
Number of pages	10
Journal	Fungal Genetics and Biology
Volume	46
Issue number	2
Early online date	6 Nov 2008
DOIs	https://doi.org/10.1016/j.fgb.2008.10.012
Publication status	Published - Feb 2009

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A paid open access option is available for this journal.

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Keywords

animals
Candida albicans
candidiasis
fungal proteins
gene expression regulation, fungal
genome, fungal
humans
kidney
oligonucleotide array sequence analysis
rabbits
virulence
transcript profiling
infection
genomics
microarrays

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10.1016/j.fgb.2008.10.012

Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney
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Cite this

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title = "Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney",

abstract = "Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.",

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note = "A paid open access option is available for this journal. Voluntary deposit by author of pre-print allowed on Institutions open scholarly website and pre-print servers Voluntary deposit by author of authors post-print allowed on institutions open scholarly website including Institutional Repository Deposit due to Funding Body, Institutional and Governmental mandate only allowed where separate agreement between repository and publisher exists Set statement to accompany deposit Published source must be acknowledged Must link to journal home page or articles' DOI Publisher's version/PDF cannot be used Articles in some journals can be made Open Access on payment of additional charge NIH Authors articles will be submitted to PubMed Central after <num>12</num> <period units={"}month{"}>months</period> Authors who are required to deposit in subject-based repositories may also use Sponsorship Option ",

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Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney

Abstract

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Keywords

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