Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney

Louise A Walker, Donna M MacCallum, Gwyneth Bertram, Neil A R Gow, Frank C Odds, Alistair J P Brown

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Abstract

Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.
Original languageEnglish
Pages (from-to)210-219
Number of pages10
JournalFungal Genetics and Biology
Volume46
Issue number2
Early online date6 Nov 2008
DOIs
Publication statusPublished - Feb 2009

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Keywords

  • animals
  • Candida albicans
  • candidiasis
  • fungal proteins
  • gene expression regulation, fungal
  • genome, fungal
  • humans
  • kidney
  • oligonucleotide array sequence analysis
  • rabbits
  • virulence
  • transcript profiling
  • infection
  • genomics
  • microarrays

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