Genomic organisation of rainbow trout, Oncorhynchus roykiss TGF-β

Garry D. Daniels, Christopher J. Secombes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


The genomic organisation of Oncorhynchus mykiss TGF-β has been determined through the generation of contiguous clones by PCR. The O. mykiss TGFβ gene is approximately 3.4 Kb in length and consists of 7 coding exons with no introns in the 5'-UTR, Whilst this is the same number of exons found in TGF-β genes of amphibians, birds and mammals, in the O. mykiss gene intron 2 of other vertebrates is absent and an additional intron is present at the 3' end of the molecule, splitting exon 7 of the other known TGF-β genes into two exons (trout exons 6 and 7). Comparison of exon sizes in the coding region support the suggestion that the Xenopus TGF-β5 and trout TGF- β sequences are the forerunners of TGF-β1. Conservation of exons coding for the mature TGF-β peptide is relatively high (63-73% identity) but other exons show lower identities (37-58%). Comparison of the TGF-β intron sequences reveals that in general the O. mykiss introns are considerably shorter than the avian homologs. The impact of the teleost TGF-β gene organisation on theories of the gene evolution of this cytokine family are discussed.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalDevelopmental and Comparative Immunology
Issue number2
Publication statusPublished - 1 Mar 1999


  • Cytokine
  • Exons
  • Gene organisation
  • Introns
  • PCR
  • Rainbow trout
  • Splice sites
  • TGF-β


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