Genomic Tools for Examining Immune Gene Function in Salmonid Fish

Samuel Allen Moore Martin, B. Collet, S. Mackenzie, O. Evensen, Christopher John Secombes

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The health status of farmed fish is central to the efficient production for harvest, as infection, either acute or chronic, will have serious effects on survival, growth, and quality of the final product. A correctly functioning immune system is vital to efficient aquaculture for both defense against invading pathogens and response to vaccines. The increase in sequence data for salmonid fish has allowed the development of tools to examine the immune response at both the transcriptional and the cellular level. This review will focus on identification of novel fish immune-related mRNA species by suppression subtractive hybridization, the expression pattern of mRNAs by transcriptome analysis (microarray), and the use of salmonid cell lines to examine the functional aspects of some key fish immune genes.

Original languageEnglish
Pages (from-to)112-118
Number of pages7
JournalReviews in Fisheries Science
Volume16
Issue numberS1
DOIs
Publication statusPublished - 2008

Keywords

  • microarray
  • subtractive cloning
  • cell lines
  • cell engineering
  • immunology
  • salmon
  • trout oncorhynchus-mykiss
  • suppression subtractive hybridization
  • rainbow-trout
  • Atlantic salmon
  • cell-line
  • CDNA microarray
  • necrosis virus
  • innate immunity
  • expression
  • salar

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