Genotype and interleukin-10 responses after cardiopulmonary bypass

Helen Frances Galley, Peter Lowe, R. L. Carmichael, Nigel Robert Webster

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Background. The pro- and anti-inflammatory cytokine balance has been implicated in outcome from inflammatory conditions, and cardiopulmonary bypass is associated with a marked inflammatory response. Interleukin-10 (IL-10) is an anti-inflammatory cytokine and levels have been shown to be highest in those patients who develop sepsis after trauma or surgery. IL-10 levels vary between individuals and genotype may dictate the IL-10 response. We therefore investigated IL-10 genotype, circulating IL-10 concentrations and outcome in terms of organ dysfunction 24 h after cardiopulmonary bypass.

Methods. Blood samples were obtained from 150 patients before, and 3, and 24 h after cardiopulmonary bypass. IL-10 was measured by enzyme immunoassay. The single nucleotide polymorphism at -1082 base pairs was detected by restriction fragment length polymorphism analysis. Post-bypass organ system dysfunction was defined prospectively.

Results. IL-10 concentrations were increased 3 h after bypass (P<0.0001) and were still increased at 24 h (P<0.0001). Homozygosity for the G allele was associated with lower median (range) maximal IL-10 levels at 3 h (44 (13-136) pg ml(-1)) compared with the A allele (118 (39-472) pg ml(-1); P=0.042). Those patients who developed at least one organ dysfunction (n=33) had higher IL-10 levels 3 h after surgery (242 (18-694) pg ml(-1)) compared with those without organ dysfunction (77 (7-586) pg ml(-1); P=0.001, n=117).

Conclusions. The G allele of the -1082 base pair single nucleotide polymorphism in the IL-10 gene is associated with lower IL-10 release after cardiopulmonary bypass. High levels of IL-10 secretion are associated with organ dysfunction 24 h after surgery.

Original languageEnglish
Pages (from-to)424-426
Number of pages2
JournalBritish Journal of Anaesthesia
Volume91
Issue number3
DOIs
Publication statusPublished - 2003

Keywords

  • complications, organ dysfunction
  • polypeptide, cytokines, interleukin-10
  • heart, cardiopulmonary bypass
  • system
  • sepsis
  • gene

Cite this

Genotype and interleukin-10 responses after cardiopulmonary bypass. / Galley, Helen Frances; Lowe, Peter; Carmichael, R. L.; Webster, Nigel Robert.

In: British Journal of Anaesthesia, Vol. 91, No. 3, 2003, p. 424-426.

Research output: Contribution to journalArticle

Galley, Helen Frances ; Lowe, Peter ; Carmichael, R. L. ; Webster, Nigel Robert. / Genotype and interleukin-10 responses after cardiopulmonary bypass. In: British Journal of Anaesthesia. 2003 ; Vol. 91, No. 3. pp. 424-426.
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abstract = "Background. The pro- and anti-inflammatory cytokine balance has been implicated in outcome from inflammatory conditions, and cardiopulmonary bypass is associated with a marked inflammatory response. Interleukin-10 (IL-10) is an anti-inflammatory cytokine and levels have been shown to be highest in those patients who develop sepsis after trauma or surgery. IL-10 levels vary between individuals and genotype may dictate the IL-10 response. We therefore investigated IL-10 genotype, circulating IL-10 concentrations and outcome in terms of organ dysfunction 24 h after cardiopulmonary bypass.Methods. Blood samples were obtained from 150 patients before, and 3, and 24 h after cardiopulmonary bypass. IL-10 was measured by enzyme immunoassay. The single nucleotide polymorphism at -1082 base pairs was detected by restriction fragment length polymorphism analysis. Post-bypass organ system dysfunction was defined prospectively.Results. IL-10 concentrations were increased 3 h after bypass (P<0.0001) and were still increased at 24 h (P<0.0001). Homozygosity for the G allele was associated with lower median (range) maximal IL-10 levels at 3 h (44 (13-136) pg ml(-1)) compared with the A allele (118 (39-472) pg ml(-1); P=0.042). Those patients who developed at least one organ dysfunction (n=33) had higher IL-10 levels 3 h after surgery (242 (18-694) pg ml(-1)) compared with those without organ dysfunction (77 (7-586) pg ml(-1); P=0.001, n=117).Conclusions. The G allele of the -1082 base pair single nucleotide polymorphism in the IL-10 gene is associated with lower IL-10 release after cardiopulmonary bypass. High levels of IL-10 secretion are associated with organ dysfunction 24 h after surgery.",
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T1 - Genotype and interleukin-10 responses after cardiopulmonary bypass

AU - Galley, Helen Frances

AU - Lowe, Peter

AU - Carmichael, R. L.

AU - Webster, Nigel Robert

PY - 2003

Y1 - 2003

N2 - Background. The pro- and anti-inflammatory cytokine balance has been implicated in outcome from inflammatory conditions, and cardiopulmonary bypass is associated with a marked inflammatory response. Interleukin-10 (IL-10) is an anti-inflammatory cytokine and levels have been shown to be highest in those patients who develop sepsis after trauma or surgery. IL-10 levels vary between individuals and genotype may dictate the IL-10 response. We therefore investigated IL-10 genotype, circulating IL-10 concentrations and outcome in terms of organ dysfunction 24 h after cardiopulmonary bypass.Methods. Blood samples were obtained from 150 patients before, and 3, and 24 h after cardiopulmonary bypass. IL-10 was measured by enzyme immunoassay. The single nucleotide polymorphism at -1082 base pairs was detected by restriction fragment length polymorphism analysis. Post-bypass organ system dysfunction was defined prospectively.Results. IL-10 concentrations were increased 3 h after bypass (P<0.0001) and were still increased at 24 h (P<0.0001). Homozygosity for the G allele was associated with lower median (range) maximal IL-10 levels at 3 h (44 (13-136) pg ml(-1)) compared with the A allele (118 (39-472) pg ml(-1); P=0.042). Those patients who developed at least one organ dysfunction (n=33) had higher IL-10 levels 3 h after surgery (242 (18-694) pg ml(-1)) compared with those without organ dysfunction (77 (7-586) pg ml(-1); P=0.001, n=117).Conclusions. The G allele of the -1082 base pair single nucleotide polymorphism in the IL-10 gene is associated with lower IL-10 release after cardiopulmonary bypass. High levels of IL-10 secretion are associated with organ dysfunction 24 h after surgery.

AB - Background. The pro- and anti-inflammatory cytokine balance has been implicated in outcome from inflammatory conditions, and cardiopulmonary bypass is associated with a marked inflammatory response. Interleukin-10 (IL-10) is an anti-inflammatory cytokine and levels have been shown to be highest in those patients who develop sepsis after trauma or surgery. IL-10 levels vary between individuals and genotype may dictate the IL-10 response. We therefore investigated IL-10 genotype, circulating IL-10 concentrations and outcome in terms of organ dysfunction 24 h after cardiopulmonary bypass.Methods. Blood samples were obtained from 150 patients before, and 3, and 24 h after cardiopulmonary bypass. IL-10 was measured by enzyme immunoassay. The single nucleotide polymorphism at -1082 base pairs was detected by restriction fragment length polymorphism analysis. Post-bypass organ system dysfunction was defined prospectively.Results. IL-10 concentrations were increased 3 h after bypass (P<0.0001) and were still increased at 24 h (P<0.0001). Homozygosity for the G allele was associated with lower median (range) maximal IL-10 levels at 3 h (44 (13-136) pg ml(-1)) compared with the A allele (118 (39-472) pg ml(-1); P=0.042). Those patients who developed at least one organ dysfunction (n=33) had higher IL-10 levels 3 h after surgery (242 (18-694) pg ml(-1)) compared with those without organ dysfunction (77 (7-586) pg ml(-1); P=0.001, n=117).Conclusions. The G allele of the -1082 base pair single nucleotide polymorphism in the IL-10 gene is associated with lower IL-10 release after cardiopulmonary bypass. High levels of IL-10 secretion are associated with organ dysfunction 24 h after surgery.

KW - complications, organ dysfunction

KW - polypeptide, cytokines, interleukin-10

KW - heart, cardiopulmonary bypass

KW - system

KW - sepsis

KW - gene

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DO - 10.1093/bja/aeg174

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VL - 91

SP - 424

EP - 426

JO - British Journal of Anaesthesia

JF - British Journal of Anaesthesia

SN - 0007-0912

IS - 3

ER -