Genotypic and phenotypic correlates with proliferative kidney disease-induced mortality in Atlantic salmon

Eef Cauwelier, John Gilbey, Catherine Jones, Leslie Noble, Eric Verspoor

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Heritable variation in resistance to pathogens has been reported in many fish species, but little is known about its genetic architecture. To extend understanding, an investigation was made of the association of resistance to proliferative kidney disease (PKD) in 4 second filial generation (F-2) families of Atlantic salmon with molecular markers from different genetic linkage groups in the species' genome, following a natural disease outbreak. PKD causes serious mortality in cultured salmonids. In addition to mortality, associations with growth-related traits were also examined, as immune responses are energetically costly and have been observed to reduce growth. Associations were investigated for 34 microsatellite markers and 5 restriction fragment length polymorphism (RFLP) loci from 3 regions of the growth hormone 1 gene (GH1). The phenotypic and genotypic character of survivors was compared with unexposed fish derived from the same families. Mortality was not size-selective, but growth in the survivors was reduced, and fish had a lower condition factor than unexposed fish, suggesting an energetic cost to resistance. Five markers showed significant allele frequency differences between survivors and unexposed fish, albeit in single families. Prior to correction for multiple tests, 2 of these markers were also linked to variation in growth-related traits among survivors, along with a further 7 markers. Though sample sizes constrained the power of the analysis, the study points to regions of the salmon genome that may contain quantitative trait loci related to PKD resistance, on which further work on the genetic architecture of PKD resistance in this species could focus.

Original languageEnglish
Pages (from-to)125-135
Number of pages11
JournalDiseases of Aquatic Organisms
Volume89
Issue number2
DOIs
Publication statusPublished - 9 Mar 2010

Keywords

  • proliferative kidney disease
  • PKD
  • Atlantic salmon
  • resistance
  • growth
  • marker-trait associations
  • quantitative trait locus
  • QTL
  • trout oncorhynchus-mykiss
  • quantitative trait loci
  • parr smolt transformation
  • genetic-variation
  • salar L
  • growth-hormone
  • linkage map
  • salvelinus-alpinus
  • trade-offs

Cite this

Genotypic and phenotypic correlates with proliferative kidney disease-induced mortality in Atlantic salmon. / Cauwelier, Eef; Gilbey, John; Jones, Catherine; Noble, Leslie; Verspoor, Eric.

In: Diseases of Aquatic Organisms, Vol. 89, No. 2, 09.03.2010, p. 125-135.

Research output: Contribution to journalArticle

Cauwelier, Eef ; Gilbey, John ; Jones, Catherine ; Noble, Leslie ; Verspoor, Eric. / Genotypic and phenotypic correlates with proliferative kidney disease-induced mortality in Atlantic salmon. In: Diseases of Aquatic Organisms. 2010 ; Vol. 89, No. 2. pp. 125-135.
@article{4011e9413bbc4f2a963a5dd02968fe7d,
title = "Genotypic and phenotypic correlates with proliferative kidney disease-induced mortality in Atlantic salmon",
abstract = "Heritable variation in resistance to pathogens has been reported in many fish species, but little is known about its genetic architecture. To extend understanding, an investigation was made of the association of resistance to proliferative kidney disease (PKD) in 4 second filial generation (F-2) families of Atlantic salmon with molecular markers from different genetic linkage groups in the species' genome, following a natural disease outbreak. PKD causes serious mortality in cultured salmonids. In addition to mortality, associations with growth-related traits were also examined, as immune responses are energetically costly and have been observed to reduce growth. Associations were investigated for 34 microsatellite markers and 5 restriction fragment length polymorphism (RFLP) loci from 3 regions of the growth hormone 1 gene (GH1). The phenotypic and genotypic character of survivors was compared with unexposed fish derived from the same families. Mortality was not size-selective, but growth in the survivors was reduced, and fish had a lower condition factor than unexposed fish, suggesting an energetic cost to resistance. Five markers showed significant allele frequency differences between survivors and unexposed fish, albeit in single families. Prior to correction for multiple tests, 2 of these markers were also linked to variation in growth-related traits among survivors, along with a further 7 markers. Though sample sizes constrained the power of the analysis, the study points to regions of the salmon genome that may contain quantitative trait loci related to PKD resistance, on which further work on the genetic architecture of PKD resistance in this species could focus.",
keywords = "proliferative kidney disease, PKD, Atlantic salmon, resistance, growth, marker-trait associations, quantitative trait locus, QTL, trout oncorhynchus-mykiss, quantitative trait loci, parr smolt transformation, genetic-variation, salar L, growth-hormone, linkage map, salvelinus-alpinus, trade-offs",
author = "Eef Cauwelier and John Gilbey and Catherine Jones and Leslie Noble and Eric Verspoor",
year = "2010",
month = "3",
day = "9",
doi = "10.3354/dao02191",
language = "English",
volume = "89",
pages = "125--135",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",
publisher = "Inter-Research",
number = "2",

}

TY - JOUR

T1 - Genotypic and phenotypic correlates with proliferative kidney disease-induced mortality in Atlantic salmon

AU - Cauwelier, Eef

AU - Gilbey, John

AU - Jones, Catherine

AU - Noble, Leslie

AU - Verspoor, Eric

PY - 2010/3/9

Y1 - 2010/3/9

N2 - Heritable variation in resistance to pathogens has been reported in many fish species, but little is known about its genetic architecture. To extend understanding, an investigation was made of the association of resistance to proliferative kidney disease (PKD) in 4 second filial generation (F-2) families of Atlantic salmon with molecular markers from different genetic linkage groups in the species' genome, following a natural disease outbreak. PKD causes serious mortality in cultured salmonids. In addition to mortality, associations with growth-related traits were also examined, as immune responses are energetically costly and have been observed to reduce growth. Associations were investigated for 34 microsatellite markers and 5 restriction fragment length polymorphism (RFLP) loci from 3 regions of the growth hormone 1 gene (GH1). The phenotypic and genotypic character of survivors was compared with unexposed fish derived from the same families. Mortality was not size-selective, but growth in the survivors was reduced, and fish had a lower condition factor than unexposed fish, suggesting an energetic cost to resistance. Five markers showed significant allele frequency differences between survivors and unexposed fish, albeit in single families. Prior to correction for multiple tests, 2 of these markers were also linked to variation in growth-related traits among survivors, along with a further 7 markers. Though sample sizes constrained the power of the analysis, the study points to regions of the salmon genome that may contain quantitative trait loci related to PKD resistance, on which further work on the genetic architecture of PKD resistance in this species could focus.

AB - Heritable variation in resistance to pathogens has been reported in many fish species, but little is known about its genetic architecture. To extend understanding, an investigation was made of the association of resistance to proliferative kidney disease (PKD) in 4 second filial generation (F-2) families of Atlantic salmon with molecular markers from different genetic linkage groups in the species' genome, following a natural disease outbreak. PKD causes serious mortality in cultured salmonids. In addition to mortality, associations with growth-related traits were also examined, as immune responses are energetically costly and have been observed to reduce growth. Associations were investigated for 34 microsatellite markers and 5 restriction fragment length polymorphism (RFLP) loci from 3 regions of the growth hormone 1 gene (GH1). The phenotypic and genotypic character of survivors was compared with unexposed fish derived from the same families. Mortality was not size-selective, but growth in the survivors was reduced, and fish had a lower condition factor than unexposed fish, suggesting an energetic cost to resistance. Five markers showed significant allele frequency differences between survivors and unexposed fish, albeit in single families. Prior to correction for multiple tests, 2 of these markers were also linked to variation in growth-related traits among survivors, along with a further 7 markers. Though sample sizes constrained the power of the analysis, the study points to regions of the salmon genome that may contain quantitative trait loci related to PKD resistance, on which further work on the genetic architecture of PKD resistance in this species could focus.

KW - proliferative kidney disease

KW - PKD

KW - Atlantic salmon

KW - resistance

KW - growth

KW - marker-trait associations

KW - quantitative trait locus

KW - QTL

KW - trout oncorhynchus-mykiss

KW - quantitative trait loci

KW - parr smolt transformation

KW - genetic-variation

KW - salar L

KW - growth-hormone

KW - linkage map

KW - salvelinus-alpinus

KW - trade-offs

U2 - 10.3354/dao02191

DO - 10.3354/dao02191

M3 - Article

VL - 89

SP - 125

EP - 135

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 2

ER -