Abstract
Silicon in rice (Oryza sativa L.) has been demonstrated to be involved in resistance to lodging, drought, and salinity, and also enhances resistance to pests and diseases. The aim of this study was to determine the range of silicon concentration in a set of rice (Oryza sativa L.) accessions, and to determine if the natural variation of shoot silicon is linked to the previously identified silicon transporters (Lsi genes). Silicon concentration was determined in 50 field-grown accessions, representing all sub-populations of rice, with all accessions being genotyped with 700K SNPs. SNPs within 10 kb of the Lsi genes were examined to determine if any were significantly linked with the phenotypic variation. An XRF method of silicon determination compared favourably with digestion and colorimetric analysis. There were significant genotypic differences in shoot silicon ranging from 16.5 to 42.4 mg g −1 of plant dry weight, but there was no significant difference between the rice sub-populations. Plants with different alleles for SNPs representing Lsi2 and Lsi3 were significantly different for shoot silicon concentration. Shoot silicon correlated negatively with grain arsenic in the tropical and temperate japonica sub-population, suggesting that accessions with high shoot silicon have reduced grain arsenic. This study indicates that alleles for Lsi genes are excellent candidate genes for further study to explain the natural variation of shoot silicon in rice.
| Original language | English |
|---|---|
| Pages (from-to) | 265-276 |
| Number of pages | 12 |
| Journal | Journal of Plant Nutrition and Soil Science |
| Volume | 182 |
| Issue number | 2 |
| Early online date | 19 Feb 2019 |
| DOIs | |
| Publication status | Published - Apr 2019 |
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Keywords
- arsenic
- natural variation
- Oryza sativa
- silicon
- XRF
ASJC Scopus subject areas
- Soil Science
- Plant Science
Cite this
Genotypic differences in shoot silicon concentration and the impact on grain arsenic concentration in rice. / Talukdar, Partha (Corresponding Author); Hartley, Sue E.; Travis, Anthony J.; Price, Adam H.; Norton, Gareth J.
In: Journal of Plant Nutrition and Soil Science, Vol. 182, No. 2, 04.2019, p. 265-276.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genotypic differences in shoot silicon concentration and the impact on grain arsenic concentration in rice
AU - Talukdar, Partha
AU - Hartley, Sue E.
AU - Travis, Anthony J.
AU - Price, Adam H.
AU - Norton, Gareth J.
N1 - Funded by School of Biological Sciences (SBS)
PY - 2019/4
Y1 - 2019/4
N2 - Silicon in rice (Oryza sativa L.) has been demonstrated to be involved in resistance to lodging, drought, and salinity, and also enhances resistance to pests and diseases. The aim of this study was to determine the range of silicon concentration in a set of rice (Oryza sativa L.) accessions, and to determine if the natural variation of shoot silicon is linked to the previously identified silicon transporters (Lsi genes). Silicon concentration was determined in 50 field-grown accessions, representing all sub-populations of rice, with all accessions being genotyped with 700K SNPs. SNPs within 10 kb of the Lsi genes were examined to determine if any were significantly linked with the phenotypic variation. An XRF method of silicon determination compared favourably with digestion and colorimetric analysis. There were significant genotypic differences in shoot silicon ranging from 16.5 to 42.4 mg g −1 of plant dry weight, but there was no significant difference between the rice sub-populations. Plants with different alleles for SNPs representing Lsi2 and Lsi3 were significantly different for shoot silicon concentration. Shoot silicon correlated negatively with grain arsenic in the tropical and temperate japonica sub-population, suggesting that accessions with high shoot silicon have reduced grain arsenic. This study indicates that alleles for Lsi genes are excellent candidate genes for further study to explain the natural variation of shoot silicon in rice.
AB - Silicon in rice (Oryza sativa L.) has been demonstrated to be involved in resistance to lodging, drought, and salinity, and also enhances resistance to pests and diseases. The aim of this study was to determine the range of silicon concentration in a set of rice (Oryza sativa L.) accessions, and to determine if the natural variation of shoot silicon is linked to the previously identified silicon transporters (Lsi genes). Silicon concentration was determined in 50 field-grown accessions, representing all sub-populations of rice, with all accessions being genotyped with 700K SNPs. SNPs within 10 kb of the Lsi genes were examined to determine if any were significantly linked with the phenotypic variation. An XRF method of silicon determination compared favourably with digestion and colorimetric analysis. There were significant genotypic differences in shoot silicon ranging from 16.5 to 42.4 mg g −1 of plant dry weight, but there was no significant difference between the rice sub-populations. Plants with different alleles for SNPs representing Lsi2 and Lsi3 were significantly different for shoot silicon concentration. Shoot silicon correlated negatively with grain arsenic in the tropical and temperate japonica sub-population, suggesting that accessions with high shoot silicon have reduced grain arsenic. This study indicates that alleles for Lsi genes are excellent candidate genes for further study to explain the natural variation of shoot silicon in rice.
KW - arsenic
KW - natural variation
KW - Oryza sativa
KW - silicon
KW - XRF
UR - http://www.scopus.com/inward/record.url?scp=85061899545&partnerID=8YFLogxK
U2 - 10.1002/jpln.201800373
DO - 10.1002/jpln.201800373
M3 - Article
VL - 182
SP - 265
EP - 276
JO - Journal of Plant Nutrition and Soil Science
JF - Journal of Plant Nutrition and Soil Science
SN - 1436-8730
IS - 2
ER -